Experiment set1IT036 for Pseudomonas fluorescens FW300-N2E2

LB with Cobalt chloride hexahydrate 0.16 mM

Group: stress
Media: LB + Cobalt chloride hexahydrate (0.16 mM)
Culturing: pseudo6_N2E2_ML5, 48 well microplate; Tecan Infinite F200, Aerobic, at 25 (C), shaken=orbital
By: Mark on 3/9/2015
Media components: 10 g/L Tryptone, 5 g/L Yeast Extract, 5 g/L Sodium Chloride
Growth plate: 1181 B1,B2

Specific Phenotypes

For 2 genes in this experiment

For stress Cobalt chloride hexahydrate in Pseudomonas fluorescens FW300-N2E2

For stress Cobalt chloride hexahydrate across organisms

SEED Subsystems

Subsystem	#Specific
Conserved gene cluster possibly involved in RNA metabolism	1
Cysteine Biosynthesis	1
Glycine and Serine Utilization	1
Methionine Biosynthesis	1
Pyruvate Alanine Serine Interconversions	1
Threonine anaerobic catabolism gene cluster	1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

• Cysteine metabolism
• Sulfur metabolism

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway	#Steps	#Present	#Specific
L-cysteine biosynthesis I	2	2	1
L-cysteine biosynthesis VII (from S-sulfo-L-cysteine)	4	3	1
N-3-oxalyl-L-2,3-diaminopropanoate biosynthesis	4	1	1
seleno-amino acid biosynthesis (plants)	5	3	1
D-cycloserine biosynthesis	6	1	1
L-cysteine biosynthesis VI (reverse transsulfuration)	7	3	1
superpathway of sulfate assimilation and cysteine biosynthesis	9	9	1
superpathway of seleno-compound metabolism	19	6	1