Experiment set1IT035 for Pedobacter sp. GW460-11-11-14-LB5

R2A with Nickel (II) chloride hexahydrate 0.3 mM

Group: stress
Media: R2A + Nickel (II) chloride hexahydrate (0.3 mM)
Culturing: Pedo557_ML3, 24-well transparent microplate; Multitron, Aerobic, at 30 (C), shaken=700 rpm
Growth: about 4.7 generations
By: Adam on 17-May-17
Media components: 0.5 g/L Bacto Peptone, 0.5 g/L casamino acids, 0.5 g/L Yeast Extract, 0.5 g/L D-Glucose, 0.5 g/L Starch, 0.3 g/L Potassium phosphate dibasic, 0.05 g/L Magnesium Sulfate Heptahydrate, 0.3 g/L Sodium pyruvate
Growth plate: 1754 A2

Specific Phenotypes

For 15 genes in this experiment

For stress Nickel (II) chloride hexahydrate in Pedobacter sp. GW460-11-11-14-LB5

For stress Nickel (II) chloride hexahydrate across organisms

SEED Subsystems

Subsystem	#Specific
Cobalt-zinc-cadmium resistance	2
Multidrug Resistance Efflux Pumps	2
Copper homeostasis: copper tolerance	1
Histidine Degradation	1
Inositol catabolism	1
Multidrug efflux pump in Campylobacter jejuni (CmeABC operon)	1
Phosphate metabolism	1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

• Histidine metabolism
• Inositol phosphate metabolism

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway	#Steps	#Present	#Specific
L-histidine degradation I	4	3	1
L-histidine degradation II	5	2	1
L-histidine degradation III	6	3	1
myo-inositol degradation I	7	2	1
L-histidine degradation VI	8	6	1
myo-, chiro- and scyllo-inositol degradation	10	2	1