Experiment set1IT032 for Pseudomonas fluorescens FW300-N1B4

Compare to:

Sodium D-Lactate carbon source

200 most detrimental genes:

  gene name fitness t score description  
Pf1N1B4_3312 +2.6 11.9 Tricarboxylate transport protein TctB compare
Pf1N1B4_3311 +2.3 15.4 Tricarboxylate transport membrane protein TctA compare
Pf1N1B4_2284 +2.1 10.7 FIG001592: Phosphocarrier protein kinase/phosphorylase, nitrogen regulation associated compare
Pf1N1B4_2114 +1.7 10.1 DNA-directed RNA polymerase omega subunit (EC 2.7.7.6) compare
Pf1N1B4_3501 +1.5 9.6 BarA sensory histidine kinase (= VarS = GacS) compare
Pf1N1B4_290 +1.4 12.7 Glutamate synthase [NADPH] large chain (EC 1.4.1.13) compare
Pf1N1B4_2060 +1.3 3.6 hypothetical protein compare
Pf1N1B4_3115 +1.3 8.6 RNA polymerase sigma factor RpoS compare
Pf1N1B4_2401 +1.2 3.6 Lipoprotein, putative compare
Pf1N1B4_5357 +1.2 2.4 hypothetical protein compare
Pf1N1B4_1185 +1.2 6.8 Lactate-responsive regulator LldR in Enterobacteria, GntR family compare
Pf1N1B4_3582 +1.1 1.8 C-methyltransferase compare
Pf1N1B4_3314 +1.1 6.0 Tricarboxylate porin OpdH compare
Pf1N1B4_4358 +1.0 2.0 Acetyltransferase, GNAT family compare
Pf1N1B4_4054 +1.0 5.3 ATP-dependent Clp protease adaptor protein ClpS compare
Pf1N1B4_5365 +0.9 2.4 hypothetical protein compare
Pf1N1B4_4687 +0.9 5.3 Glycogen synthase, ADP-glucose transglucosylase (EC 2.4.1.21) compare
Pf1N1B4_4855 +0.9 2.4 Response regulator compare
Pf1N1B4_5055 +0.9 3.4 (R)-3-hydroxydecanoyl-ACP:CoA transacylase PhaG (3-hydroxyacyl-CoA-acyl carrier protein transferase) (EC 2.4.1.-) compare
Pf1N1B4_4509 +0.9 2.0 Aldose 1-epimerase compare
Pf1N1B4_3906 +0.9 3.0 DNA topoisomerase I (EC 5.99.1.2) compare
Pf1N1B4_218 +0.9 5.5 Periplasmic binding protein compare
Pf1N1B4_5797 +0.9 2.5 1,2-dihydroxycyclohexa-3,5-diene-1-carboxylate dehydrogenase (EC 1.3.1.25) compare
Pf1N1B4_5331 +0.9 1.6 hypothetical protein compare
Pf1N1B4_2227 +0.9 2.3 FIG019278: hypothetical protein compare
Pf1N1B4_608 +0.9 2.8 Heme oxygenase HemO, associated with heme uptake compare
Pf1N1B4_4758 +0.8 3.0 Threonine dehydratase biosynthetic (EC 4.3.1.19) compare
Pf1N1B4_3794 +0.8 2.5 hypothetical protein compare
Pf1N1B4_287 +0.8 4.6 Possible periplasmic thiredoxin compare
Pf1N1B4_5507 +0.8 1.9 hypothetical protein compare
Pf1N1B4_1511 +0.8 1.6 FIG027190: Putative transmembrane protein compare
Pf1N1B4_855 +0.8 2.3 FIG021952: putative membrane protein compare
Pf1N1B4_2435 +0.8 1.8 hypothetical protein compare
Pf1N1B4_1334 +0.8 1.6 FIG00955840: hypothetical protein compare
Pf1N1B4_1888 +0.8 3.5 Glutamate-aspartate carrier protein compare
Pf1N1B4_128 +0.8 1.8 Methylglyoxal reductase, acetol producing (EC 1.1.1.-) / 2,5-diketo-D-gluconic acid reductase B (EC 1.1.1.274) compare
Pf1N1B4_2411 +0.8 2.4 FIG00953568: hypothetical protein compare
Pf1N1B4_2969 +0.8 6.5 ATPase, AFG1 family compare
Pf1N1B4_5817 +0.8 2.0 Decarboxylase family protein compare
Pf1N1B4_740 +0.8 1.8 Leucyl-tRNA synthetase compare
Pf1N1B4_5560 +0.8 1.6 hypothetical protein compare
Pf1N1B4_2635 +0.8 2.1 hypothetical protein compare
Pf1N1B4_5093 +0.8 2.0 Endo-beta-1,3-glucanase compare
Pf1N1B4_5294 +0.7 1.4 hypothetical protein compare
Pf1N1B4_1557 +0.7 6.1 Polyhydroxyalkanoic acid synthase compare
Pf1N1B4_3020 +0.7 3.3 Single-stranded-DNA-specific exonuclease RecJ (EC 3.1.-.-) compare
Pf1N1B4_3439 +0.7 1.7 hypothetical protein compare
Pf1N1B4_676 +0.7 2.1 FIG00953991: hypothetical protein compare
Pf1N1B4_2244 +0.7 1.7 Taurine-binding periplasmic protein TauA compare
Pf1N1B4_5447 +0.7 1.7 hypothetical protein compare
Pf1N1B4_538 +0.7 2.3 hypothetical protein compare
Pf1N1B4_2106 +0.7 2.5 Hydrogen peroxide-inducible genes activator compare
Pf1N1B4_1885 +0.7 4.3 alginate biosynthesis transcriptional regulatory protein AlgB compare
Pf1N1B4_5574 +0.7 1.8 hypothetical protein compare
Pf1N1B4_3836 +0.7 1.9 Nitrite reductase [NAD(P)H] small subunit (EC 1.7.1.4) compare
Pf1N1B4_3787 +0.7 2.5 FIG00960829: hypothetical protein compare
Pf1N1B4_5283 +0.7 1.8 hypothetical protein compare
Pf1N1B4_2026 +0.7 2.5 Transcriptional regulator compare
Pf1N1B4_5619 +0.7 3.7 BarA-associated response regulator UvrY (= GacA = SirA) compare
Pf1N1B4_4480 +0.7 2.8 Branched-chain alpha-keto acid dehydrogenase, E1 component, alpha subunit (EC 1.2.4.4) compare
Pf1N1B4_5610 +0.7 2.3 Probable acyl-CoA dehydrogenase (EC 1.3.99.3) compare
Pf1N1B4_5095 +0.7 2.1 hypothetical protein compare
Pf1N1B4_3563 +0.7 1.8 hypothetical protein compare
Pf1N1B4_286 +0.7 5.0 2-dehydropantoate 2-reductase (EC 1.1.1.169) compare
Pf1N1B4_919 +0.7 1.6 hypothetical protein compare
Pf1N1B4_1475 +0.7 3.6 FIG00962376: hypothetical protein compare
Pf1N1B4_1886 +0.7 3.0 FIG00956005: hypothetical protein compare
Pf1N1B4_4132 +0.7 1.5 Mobile element protein compare
Pf1N1B4_2853 +0.6 1.4 Pantoate--beta-alanine ligase (EC 6.3.2.1) compare
Pf1N1B4_5650 +0.6 2.3 Phosphatidylserine/phosphatidylglycerophosphate/ cardiolipi n synthases and related enzymes compare
Pf1N1B4_263 +0.6 1.9 Thiopurine S-methyltransferase (EC 2.1.1.67) compare
Pf1N1B4_5082 +0.6 2.2 Capsular polysaccharide synthesis enzyme CpsB compare
Pf1N1B4_3519 +0.6 2.2 Lipase (EC 3.1.1.3) compare
Pf1N1B4_2642 +0.6 1.7 Methionine ABC transporter ATP-binding protein compare
Pf1N1B4_3804 +0.6 2.2 Glycine-rich cell wall structural protein precursor compare
Pf1N1B4_4863 +0.6 2.8 Outer membrane protein OprN compare
Pf1N1B4_3263 +0.6 2.8 FIG126011: Hypothetical protein compare
Pf1N1B4_4657 +0.6 1.5 Oxidoreductase probably involved in sulfite reduction compare
Pf1N1B4_1670 +0.6 2.2 FIG001957: putative hydrolase compare
Pf1N1B4_733 +0.6 1.4 Methionyl-tRNA synthetase (EC 6.1.1.10) compare
Pf1N1B4_507 +0.6 0.9 FIG00956448: hypothetical protein compare
Pf1N1B4_4089 +0.6 1.1 hypothetical protein compare
Pf1N1B4_3637 +0.6 2.4 FlhB domain protein compare
Pf1N1B4_4869 +0.6 2.4 Isoquinoline 1-oxidoreductase beta subunit (EC 1.3.99.16) compare
Pf1N1B4_1556 +0.6 2.6 Poly(3-hydroxyalkanoate) depolymerase compare
Pf1N1B4_5746 +0.6 2.2 FIG139552: Putative protease compare
Pf1N1B4_4505 +0.6 1.7 Coenzyme PQQ synthesis protein E compare
Pf1N1B4_483 +0.6 1.4 Metal-dependent hydrolase related to alanyl-tRNA synthetase compare
Pf1N1B4_288 +0.6 4.2 FIG00958794: hypothetical protein compare
Pf1N1B4_3827 +0.6 3.7 Esterase/lipase/thioesterase family protein compare
Pf1N1B4_1963 +0.6 2.7 O-antigen export system permease protein RfbD compare
Pf1N1B4_1244 +0.6 2.7 Nucleoside ABC transporter, permease protein 1 compare
Pf1N1B4_1887 +0.6 1.9 probable exported protein YPO0432 compare
Pf1N1B4_5274 +0.6 2.1 hypothetical protein compare
Pf1N1B4_5877 +0.6 1.9 FIG00967533: hypothetical protein compare
Pf1N1B4_3634 +0.6 2.1 CheW domain protein compare
Pf1N1B4_5596 +0.6 3.2 Putative hydroxycinnamate transporter compare
Pf1N1B4_2854 +0.6 2.7 3-methyl-2-oxobutanoate hydroxymethyltransferase (EC 2.1.2.11) compare
Pf1N1B4_4408 +0.6 1.9 two-component response regulator compare
Pf1N1B4_5647 +0.6 2.3 hypothetical protein compare
Pf1N1B4_5899 +0.6 1.6 FIG00954746: hypothetical protein compare
Pf1N1B4_2382 +0.6 4.3 ATP-dependent RNA helicase RhlE compare
Pf1N1B4_2357 +0.6 3.6 type IV pilus biogenesis protein PilJ compare
Pf1N1B4_4256 +0.6 1.8 Probable transmembrane protein compare
Pf1N1B4_1492 +0.6 1.2 FIG00959437: hypothetical protein compare
Pf1N1B4_4624 +0.6 2.0 Ketoglutarate semialdehyde dehydrogenase (EC 1.2.1.26) compare
Pf1N1B4_3316 +0.6 1.9 Tricarboxylate transport transcriptional regulator TctD compare
Pf1N1B4_5496 +0.6 1.2 hypothetical protein compare
Pf1N1B4_825 +0.6 1.1 tRNA (uridine-5-oxyacetic acid methyl ester) 34 synthase compare
Pf1N1B4_3054 +0.6 1.9 FIG00953063: hypothetical protein compare
Pf1N1B4_5470 +0.6 1.8 hypothetical protein compare
Pf1N1B4_2807 +0.6 1.6 FKBP-type peptidyl-prolyl cis-trans isomerase SlpA (EC 5.2.1.8) compare
Pf1N1B4_4287 +0.6 2.4 Inositol transport system permease protein compare
Pf1N1B4_4274 +0.6 2.1 5-keto-2-deoxygluconokinase (EC 2.7.1.92) / uncharacterized domain compare
Pf1N1B4_5804 +0.6 1.8 Muconolactone isomerase (EC 5.3.3.4) compare
Pf1N1B4_3437 +0.6 3.0 Arginine pathway regulatory protein ArgR, repressor of arg regulon compare
Pf1N1B4_5601 +0.6 2.3 FIG00966546: hypothetical protein compare
Pf1N1B4_1704 +0.5 2.0 hypothetical protein compare
Pf1N1B4_629 +0.5 1.5 Queuosine Biosynthesis QueE Radical SAM compare
Pf1N1B4_5907 +0.5 2.5 cAMP-binding proteins - catabolite gene activator and regulatory subunit of cAMP-dependent protein kinases compare
Pf1N1B4_4568 +0.5 1.3 FIG00793915: hypothetical protein compare
Pf1N1B4_2210 +0.5 1.1 Glutamate transport ATP-binding protein compare
Pf1N1B4_3048 +0.5 2.7 Outer membrane lipoprotein omp16 precursor compare
Pf1N1B4_1458 +0.5 3.6 Probable transcription regulator Mig-14 compare
Pf1N1B4_5022 +0.5 1.8 hypothetical protein compare
Pf1N1B4_5854 +0.5 2.8 FIG00960144: hypothetical protein compare
Pf1N1B4_5384 +0.5 1.6 hypothetical protein compare
Pf1N1B4_121 +0.5 2.2 Membrane fusion component of tripartite multidrug resistance system compare
Pf1N1B4_3492 +0.5 1.8 DNA-3-methyladenine glycosylase (EC 3.2.2.20) compare
Pf1N1B4_3504 +0.5 1.8 Two-component sensor histidine kinase PfeS, enterobactin compare
Pf1N1B4_292 +0.5 4.9 ErfK/YbiS/YcfS/YnhG family protein compare
Pf1N1B4_1914 +0.5 2.5 Ribosomal RNA small subunit methyltransferase B (EC 2.1.1.-) compare
Pf1N1B4_1774 +0.5 3.3 Ribosomal protein S6 glutaminyl transferase compare
Pf1N1B4_3627 +0.5 1.1 Chemotaxis regulator - transmits chemoreceptor signals to flagelllar motor components CheY compare
Pf1N1B4_4937 +0.5 2.0 Two-component hybrid sensor and regulator compare
Pf1N1B4_1802 +0.5 2.4 Membrane bound c-di-GMP receptor LapD compare
Pf1N1B4_5074 +0.5 1.3 Gluconate permease compare
Pf1N1B4_298 +0.5 1.3 Transcription elongation factor GreB compare
Pf1N1B4_1500 +0.5 2.3 Chromosome (plasmid) partitioning protein ParA compare
Pf1N1B4_619 +0.5 1.1 Sigma-54 dependent transcriptional regulator/sensory box protein compare
Pf1N1B4_2464 +0.5 1.4 Ribosomal protein S6 glutaminyl transferase compare
Pf1N1B4_5262 +0.5 2.2 hypothetical protein compare
Pf1N1B4_4422 +0.5 1.0 ABC transporter amino acid-binding protein compare
Pf1N1B4_345 +0.5 1.1 Integration host factor beta subunit compare
Pf1N1B4_2085 +0.5 1.0 Uncharacterized protein similar to VCA0109 compare
Pf1N1B4_5141 +0.5 2.1 Transcriptional regulator containing an amidase domain and an AraC-type DNA-binding HTH domain compare
Pf1N1B4_1460 +0.5 3.8 Lipid A core - O-antigen ligase and related enzymes compare
Pf1N1B4_126 +0.5 1.0 Pirin compare
Pf1N1B4_5342 +0.5 1.1 hypothetical protein compare
Pf1N1B4_4268 +0.5 1.9 Mll5186 protein compare
Pf1N1B4_4999 +0.5 1.6 Sugar diacid utilization regulator SdaR compare
Pf1N1B4_5427 +0.5 2.3 C4-type zinc finger protein, DksA/TraR family compare
Pf1N1B4_2421 +0.5 1.5 L-proline glycine betaine ABC transport system permease protein ProW (TC 3.A.1.12.1) compare
Pf1N1B4_5906 +0.5 1.5 Esterase/lipase compare
Pf1N1B4_882 +0.5 1.5 carbonic anhydrase, family 3 compare
Pf1N1B4_817 +0.5 1.7 FIG00955104: hypothetical protein compare
Pf1N1B4_5448 +0.5 1.0 hypothetical protein compare
Pf1N1B4_4587 +0.5 1.2 probable dioxygenase compare
Pf1N1B4_5972 +0.5 1.5 Ribose ABC transport system, permease protein RbsC (TC 3.A.1.2.1) compare
Pf1N1B4_5224 +0.5 1.5 tRNA-Arg-CCT compare
Pf1N1B4_2406 +0.5 2.3 COG1451: Predicted metal-dependent hydrolase compare
Pf1N1B4_4979 +0.5 1.5 Transcriptional regulator, AsnC family compare
Pf1N1B4_5091 +0.5 1.8 hypothetical protein compare
Pf1N1B4_4234 +0.5 2.0 putative transport protein compare
Pf1N1B4_255 +0.5 1.8 Free methionine-(R)-sulfoxide reductase, contains GAF domain compare
Pf1N1B4_5725 +0.5 1.2 High-affinity iron permease compare
Pf1N1B4_614 +0.5 1.7 Arginine/ornithine antiporter ArcD compare
Pf1N1B4_4918 +0.5 1.2 Predicted enzyme of the cupin superfamily compare
Pf1N1B4_3892 +0.5 2.2 Multi antimicrobial extrusion protein (Na(+)/drug antiporter), MATE family of MDR efflux pumps compare
Pf1N1B4_3818 +0.5 1.2 Propionate catabolism operon transcriptional regulator of GntR family [predicted] compare
Pf1N1B4_2390 +0.5 1.6 probable membrane protein NMA1176 compare
Pf1N1B4_260 +0.5 1.3 Peptide methionine sulfoxide reductase MsrB (EC 1.8.4.12) compare
Pf1N1B4_4180 +0.5 2.2 Phosphoribosyl transferase domain protein compare
Pf1N1B4_5261 +0.5 0.8 hypothetical protein compare
Pf1N1B4_1754 +0.5 1.7 Putative stomatin/prohibitin-family membrane protease subunit aq_911 compare
Pf1N1B4_205 +0.5 1.7 Pyoverdin biosynthesis protein PvdH, L-2,4-diaminobutyrate:2-oxoglutarate aminotransferase (EC 2.6.1.76) compare
Pf1N1B4_4104 +0.5 0.9 Probable transmembrane protein compare
Pf1N1B4_5590 +0.5 1.8 Trans-feruloyl-CoA synthase (EC 6.2.1.34) compare
Pf1N1B4_4964 +0.5 1.7 Tricarboxylate transport protein TctB compare
Pf1N1B4_1411 +0.5 1.7 GTP-binding protein HflX compare
Pf1N1B4_1265 +0.5 2.7 FIG00956297: hypothetical protein compare
Pf1N1B4_4951 +0.5 1.4 Tryptophan synthase beta chain like (EC 4.2.1.20) compare
Pf1N1B4_753 +0.5 1.4 hypothetical protein compare
Pf1N1B4_3975 +0.5 0.8 Transcriptional regulator, IclR family compare
Pf1N1B4_4057 +0.5 2.4 Arginine-tRNA-protein transferase (EC 2.3.2.8) compare
Pf1N1B4_225 +0.5 2.3 Methylcrotonyl-CoA carboxylase biotin-containing subunit (EC 6.4.1.4) compare
Pf1N1B4_1212 +0.5 1.8 Predicted flavin reductase RutF in novel pyrimidine catabolism pathway compare
Pf1N1B4_2407 +0.5 1.7 FIG00953367: hypothetical protein compare
Pf1N1B4_5726 +0.5 1.8 Ferredoxin compare
Pf1N1B4_72 +0.5 0.9 Transcriptional regulator, LysR family compare
Pf1N1B4_683 +0.5 1.7 Fumarate hydratase class I, aerobic (EC 4.2.1.2) compare
Pf1N1B4_304 +0.5 1.2 Deoxyribonuclease TatD compare
Pf1N1B4_3483 +0.5 2.0 Transcriptional regulatory protein PhoP compare
Pf1N1B4_4168 +0.5 1.2 putative membrane protein compare
Pf1N1B4_291 +0.5 3.0 Major outer membrane lipoprotein I compare
Pf1N1B4_5777 +0.5 1.0 Nitrous oxide reductase maturation protein, outer-membrane lipoprotein NosL compare
Pf1N1B4_3968 +0.5 1.5 Spermidine/putrescine-binding periplasmic protein compare
Pf1N1B4_4100 +0.5 1.3 probable transmembrane protein compare
Pf1N1B4_1456 +0.5 3.0 Hypothetical protein FIG015671 in large core OS assembly cluster compare
Pf1N1B4_3485 +0.5 1.1 hypothetical protein compare


Specific Phenotypes

For 9 genes in this experiment

For carbon source Sodium D-Lactate in Pseudomonas fluorescens FW300-N1B4

For carbon source Sodium D-Lactate across organisms