Experiment set1IT032 for Pseudomonas fluorescens FW300-N1B4

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Sodium D-Lactate carbon source

Group: carbon source
Media: RCH2_defined_noCarbon + Sodium D-Lactate (20 mM), pH=7
Culturing: pseudo1_N1B4_ML1, tube, Aerobic, at 30 (C), shaken=200 rpm
By: Jayashree on 4/23/2014
Media components: 0.25 g/L Ammonium chloride, 0.1 g/L Potassium Chloride, 0.6 g/L Sodium phosphate monobasic monohydrate, 30 mM PIPES sesquisodium salt, Wolfe's mineral mix (0.03 g/L Magnesium Sulfate Heptahydrate, 0.015 g/L Nitrilotriacetic acid, 0.01 g/L Sodium Chloride, 0.005 g/L Manganese (II) sulfate monohydrate, 0.001 g/L Cobalt chloride hexahydrate, 0.001 g/L Zinc sulfate heptahydrate, 0.001 g/L Calcium chloride dihydrate, 0.001 g/L Iron (II) sulfate heptahydrate, 0.00025 g/L Nickel (II) chloride hexahydrate, 0.0002 g/L Aluminum potassium sulfate dodecahydrate, 0.0001 g/L Copper (II) sulfate pentahydrate, 0.0001 g/L Boric Acid, 0.0001 g/L Sodium Molybdate Dihydrate, 0.003 mg/L Sodium selenite pentahydrate), Wolfe's vitamin mix (0.1 mg/L Pyridoxine HCl, 0.05 mg/L 4-Aminobenzoic acid, 0.05 mg/L Lipoic acid, 0.05 mg/L Nicotinic Acid, 0.05 mg/L Riboflavin, 0.05 mg/L Thiamine HCl, 0.05 mg/L calcium pantothenate, 0.02 mg/L biotin, 0.02 mg/L Folic Acid, 0.001 mg/L Cyanocobalamin)

Specific Phenotypes

For 9 genes in this experiment

For carbon source Sodium D-Lactate in Pseudomonas fluorescens FW300-N1B4

For carbon source Sodium D-Lactate across organisms

SEED Subsystems

Subsystem #Specific
Lactate utilization 4
L-rhamnose utilization 1
Murein hydrolase regulation and cell death 1
Terminal cytochrome C oxidases 1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway #Steps #Present #Specific
pyruvate fermentation to (S)-lactate 1 1 1
arsenite to oxygen electron transfer 2 1 1
arsenite to oxygen electron transfer (via azurin) 3 1 1
pyruvate fermentation to acetate and (S)-lactate I 4 4 1
pyruvate fermentation to acetate and lactate II 4 3 1
aerobic respiration I (cytochrome c) 4 3 1
aerobic respiration II (cytochrome c) (yeast) 4 3 1
Fe(II) oxidation 6 3 1
photorespiration I 9 6 1
homolactic fermentation 12 10 1
(S)-lactate fermentation to propanoate, acetate and hydrogen 13 5 1
Bifidobacterium shunt 15 13 1
superpathway of glucose and xylose degradation 17 16 1
heterolactic fermentation 18 15 1
superpathway of anaerobic sucrose degradation 19 15 1
hexitol fermentation to lactate, formate, ethanol and acetate 19 14 1