Experiment set1IT032 for Sinorhizobium meliloti 1021

Uridine carbon source

Group: carbon source
Media: RCH2_defined_noCarbon + Uridine (20 mM), pH=7
Culturing: Smeli_ML6, 24 deep-well microplate; Multitron, Aerobic, at 30 (C), shaken=750 rpm
Growth: about 3.7 generations
By: Mark on 6/2/2015
Media components: 0.25 g/L Ammonium chloride, 0.1 g/L Potassium Chloride, 0.6 g/L Sodium phosphate monobasic monohydrate, 30 mM PIPES sesquisodium salt, Wolfe's mineral mix (0.03 g/L Magnesium Sulfate Heptahydrate, 0.015 g/L Nitrilotriacetic acid, 0.01 g/L Sodium Chloride, 0.005 g/L Manganese (II) sulfate monohydrate, 0.001 g/L Cobalt chloride hexahydrate, 0.001 g/L Zinc sulfate heptahydrate, 0.001 g/L Calcium chloride dihydrate, 0.001 g/L Iron (II) sulfate heptahydrate, 0.00025 g/L Nickel (II) chloride hexahydrate, 0.0002 g/L Aluminum potassium sulfate dodecahydrate, 0.0001 g/L Copper (II) sulfate pentahydrate, 0.0001 g/L Boric Acid, 0.0001 g/L Sodium Molybdate Dihydrate, 0.003 mg/L Sodium selenite pentahydrate), Wolfe's vitamin mix (0.1 mg/L Pyridoxine HCl, 0.05 mg/L 4-Aminobenzoic acid, 0.05 mg/L Lipoic acid, 0.05 mg/L Nicotinic Acid, 0.05 mg/L Riboflavin, 0.05 mg/L Thiamine HCl, 0.05 mg/L calcium pantothenate, 0.02 mg/L biotin, 0.02 mg/L Folic Acid, 0.001 mg/L Cyanocobalamin)
Growth plate: Smeli_carbon_plate2 C1

Specific Phenotypes

For 11 genes in this experiment

For carbon source Uridine in Sinorhizobium meliloti 1021

For carbon source Uridine across organisms

SEED Subsystems

Subsystem	#Specific
D-ribose utilization	4
Deoxyribose and Deoxynucleoside Catabolism	4
Fructose utilization	2
Isobutyryl-CoA to Propionyl-CoA Module	1
Purine conversions	1
Queuosine-Archaeosine Biosynthesis	1
Ribitol, Xylitol, Arabitol, Mannitol and Sorbitol utilization	1
Valine degradation	1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

• Inositol phosphate metabolism
• Propanoate metabolism
• Pentose phosphate pathway
• Purine metabolism
• Pyrimidine metabolism
• Alanine and aspartate metabolism
• Valine, leucine and isoleucine degradation
• beta-Alanine metabolism
• Nicotinate and nicotinamide metabolism

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway	#Steps	#Present	#Specific
β-alanine degradation II	2	2	1
adenine and adenosine salvage II	2	2	1
pyrimidine ribonucleosides salvage III	2	2	1
guanine and guanosine salvage II	2	2	1
pyrimidine ribonucleosides salvage II	2	2	1
ribose phosphorylation	2	1	1
β-alanine degradation I	2	1	1
superpathway of guanosine nucleotides degradation (plants)	6	5	2
2-deoxy-D-ribose degradation I	3	2	1
guanosine nucleotides degradation II	4	4	1
adenosine nucleotides degradation I	8	7	2
purine nucleotides degradation I (plants)	12	10	3
guanosine nucleotides degradation I	4	3	1
propanoyl-CoA degradation II	5	4	1
acrylate degradation I	5	4	1
superpathway of purines degradation in plants	18	14	3
myo-inositol degradation I	7	7	1
2,4-dinitrotoluene degradation	7	1	1
L-valine degradation I	8	5	1
superpathway of pyrimidine ribonucleosides salvage	10	8	1
myo-, chiro- and scyllo-inositol degradation	10	7	1
NAD salvage (plants)	11	7	1