Experiment set1IT032 for Pedobacter sp. GW460-11-11-14-LB5

R2A with Polymyxin B sulfate 0.016 mg/ml

Group: stress
Media: R2A + Polymyxin B sulfate (0.016 mg/ml)
Culturing: Pedo557_ML3, 24-well transparent microplate; Multitron, Aerobic, at 30 (C), shaken=700 rpm
Growth: about 5.0 generations
By: Adam on 17-May-17
Media components: 0.5 g/L Bacto Peptone, 0.5 g/L casamino acids, 0.5 g/L Yeast Extract, 0.5 g/L D-Glucose, 0.5 g/L Starch, 0.3 g/L Potassium phosphate dibasic, 0.05 g/L Magnesium Sulfate Heptahydrate, 0.3 g/L Sodium pyruvate
Growth plate: 1755 D1

Specific Phenotypes

For 3 genes in this experiment

For stress Polymyxin B sulfate in Pedobacter sp. GW460-11-11-14-LB5

For stress Polymyxin B sulfate across organisms

SEED Subsystems

Subsystem	#Specific
Glycine Biosynthesis	1
Glycine and Serine Utilization	1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

• Glycine, serine and threonine metabolism

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway	#Steps	#Present	#Specific
L-threonine degradation II	2	1	1
superpathway of L-threonine metabolism	18	12	1