Experiment set1IT031 for Caulobacter crescentus NA1000
N-Acetyl-D-Glucosamine carbon source
Group: carbon sourceMedia: M2_noCarbon + N-Acetyl-D-Glucosamine (20 mM)
Culturing: Caulo_ML2, 24-well transparent microplate; Multitron, Aerobic, at 30 (C), shaken=700 rpm
Growth: about 5.2 generations
By: Jayashree on 22-Mar-17
Media components: 1.74 g/L Disodium phosphate, 1.06 g/L Potassium phosphate monobasic, 0.5 g/L Ammonium chloride, 0.5 mM Magnesium sulfate, 0.5 mM Calcium chloride, 0.01 mM Iron (II) sulfate heptahydrate, 0.008 mM EDTA
Growth plate: 1522 C4
Specific Phenotypes
For 4 genes in this experiment
For carbon source N-Acetyl-D-Glucosamine in Caulobacter crescentus NA1000
For carbon source N-Acetyl-D-Glucosamine across organisms
SEED Subsystems
Metabolic Maps
Color code by fitness: see overview map or list of maps.
Maps containing gene(s) with specific phenotypes:
- Glycolysis / Gluconeogenesis
- Other glycan degradation
- Nucleotide sugars metabolism
- Aminosugars metabolism
- Glycosaminoglycan degradation
- Glycosphingolipid biosynthesis - globo series
- Glycosphingolipid biosynthesis - ganglio series
- Pyruvate metabolism
- Propanoate metabolism
- Reductive carboxylate cycle (CO2 fixation)
MetaCyc Pathways
Pathways that contain genes with specific phenotypes:
| Pathway | #Steps | #Present | #Specific |
|---|---|---|---|
| acetate and ATP formation from acetyl-CoA III | 1 | 1 | 1 |
| acetate conversion to acetyl-CoA | 1 | 1 | 1 |
| ethanol degradation II | 3 | 3 | 1 |
| ethanol degradation IV | 3 | 3 | 1 |
| ethanol degradation III | 3 | 2 | 1 |
| L-isoleucine biosynthesis V | 3 | 2 | 1 |
| chitin degradation II (Vibrio) | 6 | 3 | 2 |
| superpathway of acetate utilization and formation | 3 | 1 | 1 |
| chitin deacetylation | 4 | 2 | 1 |
| 2-methylcitrate cycle I | 5 | 1 | 1 |
| β-alanine biosynthesis II | 6 | 4 | 1 |
| L-isoleucine biosynthesis IV | 6 | 4 | 1 |
| superpathway of bitter acids biosynthesis | 18 | 3 | 3 |
| lupulone and humulone biosynthesis | 6 | 1 | 1 |
| adlupulone and adhumulone biosynthesis | 6 | 1 | 1 |
| colupulone and cohumulone biosynthesis | 6 | 1 | 1 |
| 2-methylcitrate cycle II | 6 | 1 | 1 |
| chitin degradation III (Serratia) | 7 | 3 | 1 |
| reductive glycine pathway of autotrophic CO2 fixation | 9 | 4 | 1 |
| cis-geranyl-CoA degradation | 9 | 1 | 1 |
| superpathway of coenzyme A biosynthesis II (plants) | 10 | 8 | 1 |
| peptidoglycan recycling II | 10 | 2 | 1 |
| peptidoglycan recycling I | 14 | 8 | 1 |