Experiment set1IT029 for Pseudomonas syringae pv. syringae B728a

KB with Nalidixic acid sodium salt 12.5 ug/ml

Group: stress
Media: KB + Nalidixic acid sodium salt (12.5 ug/ml)
Culturing: SyringaeB728a_ML2, 24 well microplate, Aerobic, at 28 (C), shaken=250 rpm
By: Tyler on 12/4/17
Media components: 10 g/L Bacto Peptone, 1.5 g/L Potassium phosphate dibasic, 15 g/L Glycerol, 0.6 g/L Magnesium sulfate

Specific Phenotypes

For 19 genes in this experiment

For stress Nalidixic acid sodium salt in Pseudomonas syringae pv. syringae B728a

For stress Nalidixic acid sodium salt across organisms

SEED Subsystems

Subsystem	#Specific
DNA repair, bacterial	2
Bacterial Cell Division	1
DNA-replication	1
DNA Repair Base Excision	1
DNA repair, bacterial DinG and relatives	1
Lipid A modifications	1
Multidrug Resistance Efflux Pumps	1
Multidrug efflux pump in Campylobacter jejuni (CmeABC operon)	1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

• Fructose and mannose metabolism
• Glycine, serine and threonine metabolism
• Benzoxazinone biosynthesis
• Nucleotide sugars metabolism
• Diterpenoid biosynthesis

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway	#Steps	#Present	#Specific
GDP-mannose biosynthesis	4	4	2
D-mannose degradation I	2	1	1
D-mannose degradation II	2	1	1
β-1,4-D-mannosyl-N-acetyl-D-glucosamine degradation	3	2	1
mannitol biosynthesis	3	1	1
Ac/N-end rule pathway	21	6	6
mannitol degradation II	4	2	1
1,5-anhydrofructose degradation	5	2	1
colanic acid building blocks biosynthesis	11	9	2
superpathway of GDP-mannose-derived O-antigen building blocks biosynthesis	14	7	2
β-(1,4)-mannan degradation	7	2	1
Arg/N-end rule pathway (eukaryotic)	14	8	1