Experiment set1IT028 for Burkholderia phytofirmans PsJN

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N-Acetyl-D-Glucosamine carbon source

Group: carbon source
Media: RCH2_defined_noCarbon + N-Acetyl-D-Glucosamine (20 mM), pH=7
Culturing: BFirm_ML3, 24 deep-well microplate; Multitron, Aerobic, at 30 (C), shaken=750 rpm
Growth: about 6.0 generations
By: Mark on 6/2/2015
Media components: 0.25 g/L Ammonium chloride, 0.1 g/L Potassium Chloride, 0.6 g/L Sodium phosphate monobasic monohydrate, 30 mM PIPES sesquisodium salt, Wolfe's mineral mix (0.03 g/L Magnesium Sulfate Heptahydrate, 0.015 g/L Nitrilotriacetic acid, 0.01 g/L Sodium Chloride, 0.005 g/L Manganese (II) sulfate monohydrate, 0.001 g/L Cobalt chloride hexahydrate, 0.001 g/L Zinc sulfate heptahydrate, 0.001 g/L Calcium chloride dihydrate, 0.001 g/L Iron (II) sulfate heptahydrate, 0.00025 g/L Nickel (II) chloride hexahydrate, 0.0002 g/L Aluminum potassium sulfate dodecahydrate, 0.0001 g/L Copper (II) sulfate pentahydrate, 0.0001 g/L Boric Acid, 0.0001 g/L Sodium Molybdate Dihydrate, 0.003 mg/L Sodium selenite pentahydrate), Wolfe's vitamin mix (0.1 mg/L Pyridoxine HCl, 0.05 mg/L 4-Aminobenzoic acid, 0.05 mg/L Lipoic acid, 0.05 mg/L Nicotinic Acid, 0.05 mg/L Riboflavin, 0.05 mg/L Thiamine HCl, 0.05 mg/L calcium pantothenate, 0.02 mg/L biotin, 0.02 mg/L Folic Acid, 0.001 mg/L Cyanocobalamin)

Specific Phenotypes

For 6 genes in this experiment

For carbon source N-Acetyl-D-Glucosamine in Burkholderia phytofirmans PsJN

For carbon source N-Acetyl-D-Glucosamine across organisms

SEED Subsystems

Subsystem #Specific
Chitin and N-acetylglucosamine utilization 3
Sialic Acid Metabolism 2
UDP-N-acetylmuramate from Fructose-6-phosphate Biosynthesis 2
Entner-Doudoroff Pathway 1
Fructose and Mannose Inducible PTS 1
Fructose utilization 1
Mannitol Utilization 1
Pentose phosphate pathway 1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway #Steps #Present #Specific
N-acetylglucosamine degradation I 2 2 2
N-acetylglucosamine degradation II 3 3 2
pentose phosphate pathway (oxidative branch) I 3 3 1
superpathway of N-acetylglucosamine, N-acetylmannosamine and N-acetylneuraminate degradation 6 3 2
chitin derivatives degradation 8 3 2
D-galactosamine and N-acetyl-D-galactosamine degradation 4 1 1
UDP-N-acetyl-D-glucosamine biosynthesis I 5 5 1
cytosolic NADPH production (yeast) 5 4 1
N-acetyl-D-galactosamine degradation 5 1 1
UDP-N-acetyl-D-glucosamine biosynthesis II 6 4 1
formaldehyde oxidation I 6 3 1
UDP-N-acetyl-D-galactosamine biosynthesis III 6 2 1
L-lysine biosynthesis VI 7 6 1
UDP-N-acetyl-D-galactosamine biosynthesis II 7 5 1
chitin degradation I (archaea) 7 1 1
pentose phosphate pathway 8 8 1
superpathway of NAD/NADP - NADH/NADPH interconversion (yeast) 8 6 1
Entner-Doudoroff pathway I 9 9 1
L-lysine biosynthesis I 9 9 1
L-lysine biosynthesis II 9 7 1
chitin biosynthesis 9 6 1
CMP-legionaminate biosynthesis I 10 2 1
O-antigen building blocks biosynthesis (E. coli) 11 11 1
superpathway of N-acetylneuraminate degradation 22 17 2
peptidoglycan recycling I 14 10 1
superpathway of L-lysine, L-threonine and L-methionine biosynthesis II 15 13 1
superpathway of glucose and xylose degradation 17 16 1
superpathway of glycolysis and the Entner-Doudoroff pathway 17 15 1
superpathway of L-lysine, L-threonine and L-methionine biosynthesis I 18 16 1
heterolactic fermentation 18 16 1
superpathway of UDP-N-acetylglucosamine-derived O-antigen building blocks biosynthesis 24 10 1
aspartate superpathway 25 23 1
superpathway of mycolyl-arabinogalactan-peptidoglycan complex biosynthesis 33 13 1