Experiment set1IT026 for Pseudomonas fluorescens FW300-N2E2

Compare to:

L-Isoleucine carbon source

200 most important genes:

  gene name fitness t score description  
Pf6N2E2_64 -7.4 -5.1 3-isopropylmalate dehydratase small subunit (EC 4.2.1.33) compare
Pf6N2E2_479 -6.9 -4.8 Dihydrolipoyllysine-residue (2-methylpropanoyl)transferase (EC 2.3.1.168) (from data) compare
Pf6N2E2_4597 -6.6 -11.8 Homoserine O-acetyltransferase (EC 2.3.1.31) compare
Pf6N2E2_5006 -6.6 -10.0 Muramoyltetrapeptide carboxypeptidase (EC 3.4.17.13) compare
Pf6N2E2_1147 -6.3 -4.3 Enoyl-CoA hydratase [valine degradation] (EC 4.2.1.17) compare
Pf6N2E2_4596 -6.3 -12.8 Homoserine O-acetyltransferase (EC 2.3.1.31) compare
Pf6N2E2_71 -6.2 -7.4 Phosphoribosylanthranilate isomerase (EC 5.3.1.24) compare
Pf6N2E2_477 -6.2 -6.0 Branched-chain amino acid aminotransferase (EC 2.6.1.42) compare
Pf6N2E2_3782 -6.1 -6.0 Phosphoribosyl-AMP cyclohydrolase (EC 3.5.4.19) compare
Pf6N2E2_2574 -6.0 -4.1 Flagellar synthesis regulator FleN compare
Pf6N2E2_481 -6.0 -5.8 branched-chain alpha-ketoacid dehydrogenase, E1 component alpha subunit (EC 1.2.4.4) (from data) conserved
Pf6N2E2_482 -6.0 -5.5 Transcriptional regulator BkdR of isoleucine and valine catabolism operon compare
Pf6N2E2_4752 -5.4 -15.4 Malate synthase G (EC 2.3.3.9) compare
Pf6N2E2_5176 -5.3 -14.2 Acetolactate synthase small subunit (EC 2.2.1.6) compare
Pf6N2E2_3842 -5.3 -6.9 Imidazoleglycerol-phosphate dehydratase (EC 4.2.1.19) compare
Pf6N2E2_480 -5.3 -17.0 3-methyl-2-oxobutanoate dehydrogenase (2-methylpropanoyl-transferring) (EC 1.2.4.4) (from data) conserved
Pf6N2E2_4826 -5.3 -9.8 Indole-3-glycerol phosphate synthase (EC 4.1.1.48) compare
Pf6N2E2_5175 -5.3 -19.9 Acetolactate synthase large subunit (EC 2.2.1.6) compare
Pf6N2E2_3252 -5.2 -11.2 Histidinol dehydrogenase (EC 1.1.1.23) compare
Pf6N2E2_4279 -5.2 -17.7 Pyruvate carboxyl transferase subunit B (EC 6.4.1.1) compare
Pf6N2E2_77 -5.2 -6.6 O-acetylhomoserine sulfhydrylase (EC 2.5.1.49) / O-succinylhomoserine sulfhydrylase (EC 2.5.1.48) compare
Pf6N2E2_4071 -5.2 -5.7 Phosphate transport system regulatory protein PhoU compare
Pf6N2E2_4077 -5.0 -4.4 cytosolic long-chain acyl-CoA thioester hydrolase family protein compare
Pf6N2E2_6064 -4.9 -8.2 2-methylaconitate isomerase compare
Pf6N2E2_3253 -4.8 -8.4 ATP phosphoribosyltransferase (EC 2.4.2.17) (from data) compare
Pf6N2E2_4047 -4.8 -8.6 Tryptophan synthase alpha chain (EC 4.2.1.20) compare
Pf6N2E2_5156 -4.7 -3.3 Two-component sensor CbrB: intrcellular carbon:nitrogen balance compare
Pf6N2E2_3170 -4.7 -14.8 2-isopropylmalate synthase (EC 2.3.3.13) compare
Pf6N2E2_4048 -4.6 -7.6 Tryptophan synthase beta chain (EC 4.2.1.20) compare
Pf6N2E2_3941 -4.5 -5.3 Adenosine (5')-pentaphospho-(5'')-adenosine pyrophosphohydrolase (EC 3.6.1.-) compare
Pf6N2E2_4825 -4.5 -12.7 Anthranilate phosphoribosyltransferase (EC 2.4.2.18) compare
Pf6N2E2_4453 -4.5 -17.0 Polyphosphate kinase (EC 2.7.4.1) compare
Pf6N2E2_5160 -4.4 -2.6 C4-type zinc finger protein, DksA/TraR family compare
Pf6N2E2_5723 -4.4 -16.9 GTP pyrophosphokinase (EC 2.7.6.5), (p)ppGpp synthetase I / Guanosine-3',5'-bis(diphosphate) 3'-pyrophosphohydrolase (EC 3.1.7.2) compare
Pf6N2E2_6063 -4.3 -12.3 2-methylcitrate dehydratase FeS dependent (EC 4.2.1.79) compare
Pf6N2E2_4803 -4.3 -2.9 Bis(5'-nucleosyl)-tetraphosphatase, symmetrical (EC 3.6.1.41) compare
Pf6N2E2_5177 -4.3 -9.4 Ketol-acid reductoisomerase (EC 1.1.1.86) compare
Pf6N2E2_3839 -4.3 -6.5 1-(5-phosphoribosyl)-5-((5-phosphoribosylamino)methylideneamino)imidazole-4-carboxamide isomerase (EC 5.3.1.16) (from data) compare
Pf6N2E2_3783 -4.1 -7.9 phosphoribosyl-ATP pyrophosphatase (EC 3.6.1.31) (from data) compare
Pf6N2E2_279 -4.1 -10.2 Isocitrate lyase (EC 4.1.3.1) compare
Pf6N2E2_4564 -4.1 -6.9 Dihydroxy-acid dehydratase (EC 4.2.1.9) compare
Pf6N2E2_66 -4.0 -8.1 3-isopropylmalate dehydrogenase (EC 1.1.1.85) compare
Pf6N2E2_4372 -3.9 -8.7 FIG00460773: hypothetical protein compare
Pf6N2E2_3661 -3.8 -7.7 hypothetical protein compare
Pf6N2E2_4278 -3.7 -14.0 Pyruvate carboxyl transferase subunit A (EC 6.4.1.1) compare
Pf6N2E2_3610 -3.6 -4.5 ATP phosphoribosyltransferase regulatory subunit (EC 2.4.2.17) compare
Pf6N2E2_3932 -3.6 -5.5 D-3-phosphoglycerate dehydrogenase (EC 1.1.1.95) compare
Pf6N2E2_2753 -3.6 -4.9 HtrA protease/chaperone protein compare
Pf6N2E2_1401 -3.6 -10.2 FIG00955631: hypothetical protein compare
Pf6N2E2_5520 -3.6 -9.6 Sensory box histidine kinase compare
Pf6N2E2_478 -3.5 -3.1 Dihydrolipoyl dehydrogenase (EC 1.8.1.4) (from data) compare
Pf6N2E2_3630 -3.5 -7.2 Phosphoserine phosphatase (EC 3.1.3.3) compare
Pf6N2E2_4638 -3.4 -6.5 5,10-methylenetetrahydrofolate reductase (EC 1.5.1.20) compare
Pf6N2E2_3869 -3.4 -11.7 Branched-chain amino acid transport protein AzlC compare
Pf6N2E2_3248 -3.4 -7.0 Probable transmembrane protein compare
Pf6N2E2_5780 -3.3 -11.4 Periplasmic protease compare
Pf6N2E2_3841 -3.3 -7.6 Imidazole glycerol phosphate synthase amidotransferase subunit (EC 2.4.2.-) compare
Pf6N2E2_3940 -3.1 -10.6 Phosphoserine phosphatase (EC 3.1.3.3) compare
Pf6N2E2_5579 -3.1 -6.6 Ornithine carbamoyltransferase (EC 2.1.3.3) compare
Pf6N2E2_5030 -3.0 -6.4 Transcriptional regulator, AsnC family compare
Pf6N2E2_2217 -2.9 -8.9 ATP-dependent Clp protease ATP-binding subunit ClpX compare
Pf6N2E2_1146 -2.9 -7.2 2-methylbutanoyl-CoA dehydrogenase (EC 1.3.8.5) (from data) conserved
Pf6N2E2_1381 -2.8 -8.9 Aldehyde dehydrogenase (EC 1.2.1.3) compare
Pf6N2E2_5728 -2.8 -3.8 Phosphoribosylglycinamide formyltransferase (EC 2.1.2.2) compare
Pf6N2E2_3060 -2.8 -3.3 Outer membrane protein H precursor compare
Pf6N2E2_2277 -2.8 -4.4 NADPH-dependent glyceraldehyde-3-phosphate dehydrogenase (EC 1.2.1.13) compare
Pf6N2E2_1400 -2.8 -11.8 Sensory box histidine kinase/response regulator compare
Pf6N2E2_2218 -2.7 -7.2 ATP-dependent Clp protease proteolytic subunit (EC 3.4.21.92) compare
Pf6N2E2_1402 -2.7 -8.1 hypothetical protein compare
Pf6N2E2_6062 -2.7 -9.6 2-methylcitrate synthase (EC 2.3.3.5) (from data) compare
Pf6N2E2_63 -2.5 -4.0 3-isopropylmalate dehydratase large subunit (EC 4.2.1.33) compare
Pf6N2E2_5430 -2.5 -10.6 Transglycosylase, Slt family compare
Pf6N2E2_2756 -2.5 -8.7 RNA polymerase sigma-H factor AlgT compare
Pf6N2E2_3062 -2.5 -4.9 Membrane-associated zinc metalloprotease compare
Pf6N2E2_5242 -2.4 -3.2 Malate:quinone oxidoreductase (EC 1.1.5.4) compare
Pf6N2E2_4277 -2.4 -9.2 LysR family transcriptional regulator PA5437 compare
Pf6N2E2_3942 -2.4 -10.9 FIG001592: Phosphocarrier protein kinase/phosphorylase, nitrogen regulation associated compare
Pf6N2E2_4759 -2.3 -12.5 Biotin synthase (EC 2.8.1.6) compare
Pf6N2E2_3460 -2.3 -2.6 Exodeoxyribonuclease V beta chain (EC 3.1.11.5) compare
Pf6N2E2_50 -2.3 -7.3 Biosynthetic Aromatic amino acid aminotransferase alpha (EC 2.6.1.57) @ Aromatic-amino-acid aminotransferase (EC 2.6.1.57) compare
Pf6N2E2_2258 -2.2 -11.7 Transaldolase (EC 2.2.1.2) compare
Pf6N2E2_3271 -2.0 -3.7 Phosphocarrier protein, nitrogen regulation associated compare
Pf6N2E2_3282 -1.8 -3.2 FIG005080: Possible exported protein compare
Pf6N2E2_4418 -1.8 -1.3 Diaminopimelate epimerase (EC 5.1.1.7) compare
Pf6N2E2_2186 -1.8 -7.3 Isovaleryl-CoA dehydrogenase (EC 1.3.8.4) compare
Pf6N2E2_302 -1.7 -6.5 Siroheme synthase / Precorrin-2 oxidase (EC 1.3.1.76) / Sirohydrochlorin ferrochelatase (EC 4.99.1.4) / Uroporphyrinogen-III methyltransferase (EC 2.1.1.107) compare
Pf6N2E2_3134 -1.7 -1.5 hypothetical protein compare
Pf6N2E2_4072 -1.7 -3.6 Phosphate transport ATP-binding protein PstB (TC 3.A.1.7.1) compare
Pf6N2E2_1143 -1.7 -6.0 Acyl-CoA synthetases (AMP-forming)/AMP-acid ligases conserved
Pf6N2E2_1932 -1.7 -6.0 Transcriptional regulator, IclR family compare
Pf6N2E2_4009 -1.7 -8.3 GGDEF domain protein compare
Pf6N2E2_2752 -1.7 -8.4 Exported zinc metalloprotease YfgC precursor compare
Pf6N2E2_4611 -1.7 -3.2 Ferric siderophore transport system, periplasmic binding protein TonB compare
Pf6N2E2_3984 -1.6 -9.0 T1SS associated transglutaminase-like cysteine proteinase LapP compare
Pf6N2E2_4511 -1.6 -8.8 glutamine synthetase family protein compare
Pf6N2E2_5219 -1.6 -9.3 FIG140336: TPR domain protein compare
Pf6N2E2_5255 -1.6 -9.2 Outer membrane stress sensor protease DegS compare
Pf6N2E2_3667 -1.6 -2.2 Heptose kinase WapQ, eukaryotic type compare
Pf6N2E2_5316 -1.5 -1.7 Periplasmic thiol:disulfide oxidoreductase DsbB, required for DsbA reoxidation compare
Pf6N2E2_3338 -1.5 -3.0 1-phosphofructokinase (EC 2.7.1.56) compare
Pf6N2E2_2073 -1.5 -8.4 Sulfite reductase [NADPH] hemoprotein beta-component (EC 1.8.1.2) compare
Pf6N2E2_5714 -1.5 -3.4 Flavoprotein WrbA compare
Pf6N2E2_4206 -1.5 -2.9 Chromosome (plasmid) partitioning protein ParB compare
Pf6N2E2_4362 -1.5 -4.2 DNA-directed RNA polymerase omega subunit (EC 2.7.7.6) compare
Pf6N2E2_180 -1.5 -3.5 Transcriptional regulator, MerR family compare
Pf6N2E2_6061 -1.4 -6.3 Methylisocitrate lyase (EC 4.1.3.30) (from data) conserved
Pf6N2E2_3885 -1.4 -2.9 3'(2'),5'-bisphosphate nucleotidase (EC 3.1.3.7) compare
Pf6N2E2_1138 -1.4 -6.6 Transcriptional regulator, AraC family conserved
Pf6N2E2_3835 -1.4 -5.2 Carboxyl-terminal protease (EC 3.4.21.102) compare
Pf6N2E2_3833 -1.4 -4.3 hypothetical protein compare
Pf6N2E2_3349 -1.4 -2.0 Polyribonucleotide nucleotidyltransferase (EC 2.7.7.8) compare
Pf6N2E2_3770 -1.3 -3.4 ATP-dependent hsl protease ATP-binding subunit HslU compare
Pf6N2E2_4272 -1.3 -3.8 putative membrane protein compare
Pf6N2E2_3834 -1.3 -4.0 Lipoprotein NlpD compare
Pf6N2E2_2588 -1.3 -2.5 Anti-sigma F factor antagonist (spoIIAA-2); Anti-sigma B factor antagonist RsbV compare
Pf6N2E2_75 -1.3 -4.0 Colicin V production protein compare
Pf6N2E2_2473 -1.3 -1.6 Major outer membrane lipoprotein I compare
Pf6N2E2_621 -1.3 -5.9 TPR domain protein in aerotolerance operon compare
Pf6N2E2_4075 -1.2 -5.0 Phosphate ABC transporter, periplasmic phosphate-binding protein PstS (TC 3.A.1.7.1) compare
Pf6N2E2_622 -1.2 -4.3 BatA (Bacteroides aerotolerance operon) compare
Pf6N2E2_4733 -1.2 -1.9 hypothetical protein compare
Pf6N2E2_4585 -1.2 -6.7 Cell division protein FtsX compare
Pf6N2E2_3192 -1.2 -4.9 tRNA:Cm32/Um32 methyltransferase compare
Pf6N2E2_5553 -1.2 -5.5 Quinolinate synthetase (EC 2.5.1.72) compare
Pf6N2E2_3763 -1.2 -1.7 hypothetical protein compare
Pf6N2E2_5345 -1.2 -6.9 LppC putative lipoprotein compare
Pf6N2E2_280 -1.1 -2.0 hypothetical protein compare
Pf6N2E2_3251 -1.1 -6.4 histidinol-phosphate aminotransferase (EC 2.6.1.9) (from data) compare
Pf6N2E2_4276 -1.1 -3.5 Phosphogluconate repressor HexR, RpiR family compare
Pf6N2E2_2922 -1.1 -2.1 hypothetical protein compare
Pf6N2E2_4074 -1.1 -7.1 Phosphate transport system permease protein PstC (TC 3.A.1.7.1) compare
Pf6N2E2_2925 -1.1 -4.3 ABC transporter for L-leucine/L-isoleucine/L-phenylalanine/D-alanine, ATPase component 1 LivG (from data) compare
Pf6N2E2_620 -1.1 -5.1 BatD compare
Pf6N2E2_4416 -1.1 -2.0 Tyrosine recombinase XerC compare
Pf6N2E2_2926 -1.1 -5.6 ABC transporter for L-leucine/L-isoleucine/L-phenylalanine/D-alanine, ATPase component 2 LivF (from data) compare
Pf6N2E2_2794 -1.1 -2.0 putative membrane protein compare
Pf6N2E2_3346 -1.1 -4.3 Quinolinate phosphoribosyltransferase [decarboxylating] (EC 2.4.2.19) compare
Pf6N2E2_624 -1.0 -5.2 hypothetical protein PA3071 compare
Pf6N2E2_1667 -1.0 -2.8 Fumarylacetoacetate hydrolase family protein compare
Pf6N2E2_2693 -1.0 -1.8 3-oxoacyl-[acyl-carrier-protein] synthase, KASIII (EC 2.3.1.180) compare
Pf6N2E2_5785 -1.0 -1.4 Phosphoserine phosphatase (EC 3.1.3.3) compare
Pf6N2E2_70 -1.0 -2.9 tRNA pseudouridine synthase A (EC 4.2.1.70) compare
Pf6N2E2_122 -1.0 -1.8 Tyrosine-protein kinase Wzc (EC 2.7.10.2) compare
Pf6N2E2_3828 -1.0 -2.2 tRNA (cytidine(34)-2'-O)-methyltransferase (EC 2.1.1.207) compare
Pf6N2E2_3461 -1.0 -1.0 Exodeoxyribonuclease V gamma chain (EC 3.1.11.5) compare
Pf6N2E2_2074 -1.0 -2.0 required for sulfate utilization, putative electron transport protein for sulfite reductase (from data) compare
Pf6N2E2_4414 -0.9 -2.3 FIG00953808: hypothetical protein compare
Pf6N2E2_625 -0.9 -2.8 MoxR-like ATPase in aerotolerance operon compare
Pf6N2E2_3578 -0.9 -6.5 Branched-chain amino acid transport system permease protein LivM (TC 3.A.1.4.1) compare
Pf6N2E2_3466 -0.9 -1.8 FIG000605: protein co-occurring with transport systems (COG1739) compare
Pf6N2E2_3576 -0.9 -6.0 Branched-chain amino acid transport ATP-binding protein LivF (TC 3.A.1.4.1) compare
Pf6N2E2_2549 -0.9 -2.0 hypothetical protein compare
Pf6N2E2_3221 -0.9 -2.5 FIG00352444: hypothetical protein compare
Pf6N2E2_6140 -0.9 -1.1 Large Subunit Ribosomal RNA; lsuRNA; LSU rRNA compare
Pf6N2E2_3579 -0.9 -6.9 High-affinity branched-chain amino acid transport system permease protein LivH (TC 3.A.1.4.1) compare
Pf6N2E2_4067 -0.9 -5.7 Phosphate regulon sensor protein PhoR (SphS) (EC 2.7.13.3) compare
Pf6N2E2_5567 -0.9 -2.7 Glutamate Aspartate transport ATP-binding protein GltL (TC 3.A.1.3.4) compare
Pf6N2E2_510 -0.9 -6.0 Predicted transcriptional regulator of the myo-inositol catabolic operon compare
Pf6N2E2_3199 -0.9 -3.6 S-adenosylmethionine:tRNA ribosyltransferase-isomerase (EC 5.-.-.-) compare
Pf6N2E2_434 -0.9 -2.5 hypothetical protein compare
Pf6N2E2_5252 -0.9 -1.8 FIG00956396: hypothetical protein compare
Pf6N2E2_3198 -0.8 -1.5 tRNA-guanine transglycosylase (EC 2.4.2.29) compare
Pf6N2E2_4478 -0.8 -3.9 FIG019278: hypothetical protein compare
Pf6N2E2_1137 -0.8 -4.8 CDP-diacylglycerol--serine O-phosphatidyltransferase (EC 2.7.8.8) compare
Pf6N2E2_5560 -0.8 -2.6 Sigma-54 dependent transcriptional regulator/sensory box protein compare
Pf6N2E2_3613 -0.8 -4.7 GTP-binding protein HflX compare
Pf6N2E2_5023 -0.8 -4.4 Thiamin-phosphate pyrophosphorylase (EC 2.5.1.3) compare
Pf6N2E2_5727 -0.8 -3.7 FIG00955360: hypothetical protein compare
Pf6N2E2_4388 -0.8 -5.7 Gamma-glutamyl-putrescine oxidase (EC1.4.3.-) compare
Pf6N2E2_638 -0.8 -2.6 4-carboxymuconolactone decarboxylase domain/alkylhydroperoxidase AhpD family core domain protein compare
Pf6N2E2_146 -0.8 -1.3 Transcriptional regulator, MarR family compare
Pf6N2E2_4494 -0.8 -2.7 Adenylate cyclase (EC 4.6.1.1) compare
Pf6N2E2_4817 -0.8 -6.9 Spermidine Putrescine ABC transporter permease component PotB (TC 3.A.1.11.1) compare
Pf6N2E2_2465 -0.8 -3.3 Cys regulon transcriptional activator CysB compare
Pf6N2E2_3616 -0.8 -5.0 DNA mismatch repair protein MutL compare
Pf6N2E2_5771 -0.8 -3.2 FKBP-type peptidyl-prolyl cis-trans isomerase FklB (EC 5.2.1.8) compare
Pf6N2E2_1211 -0.8 -1.1 Lactoylglutathione lyase compare
Pf6N2E2_2375 -0.8 -1.7 Response regulator NasT compare
Pf6N2E2_3088 -0.8 -1.6 hypothetical protein compare
Pf6N2E2_5593 -0.8 -2.0 Leucyl-tRNA synthetase compare
Pf6N2E2_4818 -0.8 -3.9 Spermidine Putrescine ABC transporter permease component potC (TC_3.A.1.11.1) compare
Pf6N2E2_5989 -0.7 -6.0 Transcriptional regulator, GntR family domain / Aspartate aminotransferase (EC 2.6.1.1) compare
Pf6N2E2_3285 -0.7 -2.9 Rod shape-determining protein MreD compare
Pf6N2E2_3264 -0.7 -1.9 Uncharacterized protein YrbK clustered with lipopolysaccharide transporters compare
Pf6N2E2_2886 -0.7 -6.3 Phosphogluconate repressor HexR, RpiR family compare
Pf6N2E2_5111 -0.7 -1.9 FKBP-type peptidyl-prolyl cis-trans isomerase SlpA (EC 5.2.1.8) compare
Pf6N2E2_4551 -0.7 -2.2 hypothetical protein compare
Pf6N2E2_895 -0.7 -2.3 Transcriptional regulator, HxlR family compare
Pf6N2E2_1696 -0.7 -1.7 Alkanesulfonates-binding protein compare
Pf6N2E2_5420 -0.7 -3.7 Cytosol aminopeptidase PepA (EC 3.4.11.1) compare
Pf6N2E2_906 -0.7 -2.0 ISPpu14, transposase Orf2 compare
Pf6N2E2_4353 -0.7 -3.5 Hydrogen peroxide-inducible genes activator compare
Pf6N2E2_3580 -0.7 -5.1 Branched-chain amino acid ABC transporter, amino acid-binding protein (TC 3.A.1.4.1) compare
Pf6N2E2_613 -0.7 -1.2 lipoprotein, putative compare
Pf6N2E2_74 -0.7 -1.3 DedD protein compare
Pf6N2E2_3577 -0.7 -3.9 ABC transporter for L-leucine/L-isoleucine/D-alanine, ATPase component 1 LivG (from data) compare
Pf6N2E2_813 -0.7 -2.7 Response regulator compare
Pf6N2E2_2487 -0.7 -0.9 FIG00956090: hypothetical protein compare
Pf6N2E2_1371 -0.7 -2.4 hypothetical protein compare
Pf6N2E2_3611 -0.7 -0.9 HflC protein compare


Specific Phenotypes

For 6 genes in this experiment

For carbon source L-Isoleucine in Pseudomonas fluorescens FW300-N2E2

For carbon source L-Isoleucine across organisms