Experiment set1IT026 for Sinorhizobium meliloti 1021

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L-Glutamine carbon source

Group: carbon source
Media: RCH2_defined_noCarbon + L-Glutamine (20 mM), pH=7
Culturing: Smeli_ML6, 24 deep-well microplate; Multitron, Aerobic, at 30 (C), shaken=750 rpm
Growth: about 4.8 generations
By: Mark on 6/2/2015
Media components: 0.25 g/L Ammonium chloride, 0.1 g/L Potassium Chloride, 0.6 g/L Sodium phosphate monobasic monohydrate, 30 mM PIPES sesquisodium salt, Wolfe's mineral mix (0.03 g/L Magnesium Sulfate Heptahydrate, 0.015 g/L Nitrilotriacetic acid, 0.01 g/L Sodium Chloride, 0.005 g/L Manganese (II) sulfate monohydrate, 0.001 g/L Cobalt chloride hexahydrate, 0.001 g/L Zinc sulfate heptahydrate, 0.001 g/L Calcium chloride dihydrate, 0.001 g/L Iron (II) sulfate heptahydrate, 0.00025 g/L Nickel (II) chloride hexahydrate, 0.0002 g/L Aluminum potassium sulfate dodecahydrate, 0.0001 g/L Copper (II) sulfate pentahydrate, 0.0001 g/L Boric Acid, 0.0001 g/L Sodium Molybdate Dihydrate, 0.003 mg/L Sodium selenite pentahydrate), Wolfe's vitamin mix (0.1 mg/L Pyridoxine HCl, 0.05 mg/L 4-Aminobenzoic acid, 0.05 mg/L Lipoic acid, 0.05 mg/L Nicotinic Acid, 0.05 mg/L Riboflavin, 0.05 mg/L Thiamine HCl, 0.05 mg/L calcium pantothenate, 0.02 mg/L biotin, 0.02 mg/L Folic Acid, 0.001 mg/L Cyanocobalamin)
Growth plate: Smeli_carbon_plate2 B1

Specific Phenotypes

For 16 genes in this experiment

For carbon source L-Glutamine in Sinorhizobium meliloti 1021

For carbon source L-Glutamine across organisms

SEED Subsystems

Subsystem #Specific
ABC transporter branched-chain amino acid (TC 3.A.1.4.1) 4
Cobalt-zinc-cadmium resistance 1
Glutamine, Glutamate, Aspartate and Asparagine Biosynthesis 1
Ketoisovalerate oxidoreductase 1
Pentose phosphate pathway 1
Pyruvate metabolism I: anaplerotic reactions, PEP 1
Pyruvate metabolism II: acetyl-CoA, acetogenesis from pyruvate 1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway #Steps #Present #Specific
L-glutamine degradation I 1 1 1
acetate conversion to acetyl-CoA 1 1 1
acetate and ATP formation from acetyl-CoA III 1 1 1
L-glutamate biosynthesis I 2 2 1
pentose phosphate pathway (oxidative branch) I 3 3 1
ethanol degradation IV 3 3 1
superpathway of acetate utilization and formation 3 3 1
ethanol degradation II 3 3 1
ammonia assimilation cycle III 3 3 1
ethanol degradation III 3 2 1
L-isoleucine biosynthesis V 3 2 1
C4 photosynthetic carbon assimilation cycle, NADP-ME type 7 4 2
glutaminyl-tRNAgln biosynthesis via transamidation 4 4 1
L-asparagine biosynthesis III (tRNA-dependent) 4 4 1
chitin deacetylation 4 3 1
2-methylcitrate cycle I 5 1 1
β-alanine biosynthesis II 6 5 1
L-isoleucine biosynthesis IV 6 4 1
methylgallate degradation 6 4 1
superpathway of bitter acids biosynthesis 18 3 3
lupulone and humulone biosynthesis 6 1 1
adlupulone and adhumulone biosynthesis 6 1 1
2-methylcitrate cycle II 6 1 1
colupulone and cohumulone biosynthesis 6 1 1
L-glutamate and L-glutamine biosynthesis 7 5 1
C4 photosynthetic carbon assimilation cycle, PEPCK type 14 8 2
pentose phosphate pathway 8 8 1
L-citrulline biosynthesis 8 6 1
protocatechuate degradation I (meta-cleavage pathway) 8 5 1
reductive glycine pathway of autotrophic CO2 fixation 9 6 1
cis-geranyl-CoA degradation 9 2 1
superpathway of coenzyme A biosynthesis II (plants) 10 9 1
superpathway of vanillin and vanillate degradation 10 5 1
superpathway of L-citrulline metabolism 12 8 1
syringate degradation 12 5 1
gluconeogenesis I 13 12 1
superpathway of glucose and xylose degradation 17 15 1