Experiment set1IT026 for Caulobacter crescentus NA1000

m-Inositol carbon source

Group: carbon source
Media: M2_noCarbon + m-Inositol (20 mM)
Culturing: Caulo_ML2, 24-well transparent microplate; Multitron, Aerobic, at 30 (C), shaken=700 rpm
Growth: about 4.3 generations
By: Jayashree on 22-Mar-17
Media components: 1.74 g/L Disodium phosphate, 1.06 g/L Potassium phosphate monobasic, 0.5 g/L Ammonium chloride, 0.5 mM Magnesium sulfate, 0.5 mM Calcium chloride, 0.01 mM Iron (II) sulfate heptahydrate, 0.008 mM EDTA
Growth plate: 1522 B3

Specific Phenotypes

For 5 genes in this experiment

For carbon source m-Inositol in Caulobacter crescentus NA1000

For carbon source m-Inositol across organisms

SEED Subsystems

Subsystem	#Specific
Inositol catabolism	2
D-ribose utilization	1
Entner-Doudoroff Pathway	1
Glycerolipid and Glycerophospholipid Metabolism in Bacteria	1
Methylglyoxal Metabolism	1
Pyruvate metabolism II: acetyl-CoA, acetogenesis from pyruvate	1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

• Urea cycle and metabolism of amino groups
• Valine, leucine and isoleucine degradation
• Arginine and proline metabolism
• Tryptophan metabolism
• Propanoate metabolism
• Butanoate metabolism
• Limonene and pinene degradation
• Glycolysis / Gluconeogenesis
• Ascorbate and aldarate metabolism
• Fatty acid metabolism
• Glutamate metabolism
• Geraniol degradation
• Lysine biosynthesis
• Lysine degradation
• Histidine metabolism
• Tyrosine metabolism
• beta-Alanine metabolism
• Glycerolipid metabolism
• Inositol phosphate metabolism
• Pyruvate metabolism
• 1- and 2-Methylnaphthalene degradation
• Naphthalene and anthracene degradation
• 1,2-Dichloroethane degradation
• 3-Chloroacrylic acid degradation

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway	#Steps	#Present	#Specific
putrescine degradation V	2	2	1
β-alanine degradation II	2	2	1
β-alanine degradation I	2	1	1
4-aminobutanoate degradation III	2	1	1
putrescine degradation I	2	1	1
ethylene glycol degradation	2	1	1
4-aminobutanoate degradation II	2	1	1
ethanol degradation IV	3	3	1
ethanol degradation II	3	3	1
putrescine degradation IV	3	2	1
hypotaurine degradation	3	2	1
ethanol degradation III	3	2	1
histamine degradation	3	1	1
superpathway of 4-aminobutanoate degradation	3	1	1
phytol degradation	4	3	1
putrescine degradation III	4	2	1
L-tryptophan degradation X (mammalian, via tryptamine)	4	2	1
fatty acid α-oxidation I (plants)	4	2	1
D-arabinose degradation II	4	1	1
propanoyl-CoA degradation II	5	3	1
mitochondrial NADPH production (yeast)	5	3	1
sphingosine and sphingosine-1-phosphate metabolism	10	4	2
dopamine degradation	5	2	1
octane oxidation	5	2	1
L-lysine degradation IV	5	1	1
superpathway of L-arginine, putrescine, and 4-aminobutanoate degradation	11	5	2
3-methyl-branched fatty acid α-oxidation	6	3	1
L-lysine degradation X	6	3	1
alkane oxidation	6	1	1
L-lysine degradation III	6	1	1
noradrenaline and adrenaline degradation	13	8	2
superpathway of L-arginine and L-ornithine degradation	13	7	2
myo-inositol degradation I	7	7	1
serotonin degradation	7	4	1
superpathway of glycol metabolism and degradation	7	3	1
L-lysine degradation I	7	3	1
ceramide degradation by α-oxidation	7	2	1
2,4-dinitrotoluene degradation	7	1	1
limonene degradation IV (anaerobic)	7	1	1
superpathway of NAD/NADP - NADH/NADPH interconversion (yeast)	8	6	1
L-valine degradation I	8	5	1
superpathway of ornithine degradation	8	5	1
aromatic biogenic amine degradation (bacteria)	8	3	1
ceramide and sphingolipid recycling and degradation (yeast)	16	4	2
4-hydroxyphenylacetate degradation	8	2	1
TCA cycle IV (2-oxoglutarate decarboxylase)	9	8	1
Entner-Doudoroff pathway II (non-phosphorylative)	9	4	1
myo-, chiro- and scyllo-inositol degradation	10	7	1
nicotine degradation II (pyrrolidine pathway)	11	2	1
nicotine degradation I (pyridine pathway)	17	4	1
superpathway of pentose and pentitol degradation	42	12	1
superpathway of L-lysine degradation	43	8	1