Experiment set1IT024 for Sinorhizobium meliloti 1021

Compare to:

L-Histidine carbon source

Group: carbon source
Media: RCH2_defined_noCarbon + L-Histidine (20 mM), pH=7
Culturing: Smeli_ML6, 24 deep-well microplate; Multitron, Aerobic, at 30 (C), shaken=750 rpm
Growth: about 3.6 generations
By: Mark on 6/2/2015
Media components: 0.25 g/L Ammonium chloride, 0.1 g/L Potassium Chloride, 0.6 g/L Sodium phosphate monobasic monohydrate, 30 mM PIPES sesquisodium salt, Wolfe's mineral mix (0.03 g/L Magnesium Sulfate Heptahydrate, 0.015 g/L Nitrilotriacetic acid, 0.01 g/L Sodium Chloride, 0.005 g/L Manganese (II) sulfate monohydrate, 0.001 g/L Cobalt chloride hexahydrate, 0.001 g/L Zinc sulfate heptahydrate, 0.001 g/L Calcium chloride dihydrate, 0.001 g/L Iron (II) sulfate heptahydrate, 0.00025 g/L Nickel (II) chloride hexahydrate, 0.0002 g/L Aluminum potassium sulfate dodecahydrate, 0.0001 g/L Copper (II) sulfate pentahydrate, 0.0001 g/L Boric Acid, 0.0001 g/L Sodium Molybdate Dihydrate, 0.003 mg/L Sodium selenite pentahydrate), Wolfe's vitamin mix (0.1 mg/L Pyridoxine HCl, 0.05 mg/L 4-Aminobenzoic acid, 0.05 mg/L Lipoic acid, 0.05 mg/L Nicotinic Acid, 0.05 mg/L Riboflavin, 0.05 mg/L Thiamine HCl, 0.05 mg/L calcium pantothenate, 0.02 mg/L biotin, 0.02 mg/L Folic Acid, 0.001 mg/L Cyanocobalamin)
Growth plate: Smeli_carbon_plate2 A4

Specific Phenotypes

For 24 genes in this experiment

For carbon source L-Histidine in Sinorhizobium meliloti 1021

For carbon source L-Histidine across organisms

SEED Subsystems

Subsystem #Specific
Histidine Degradation 6
ABC transporter branched-chain amino acid (TC 3.A.1.4.1) 4
Pyruvate metabolism II: acetyl-CoA, acetogenesis from pyruvate 2
Autoinducer 2 (AI-2) transport and processing (lsrACDBFGE operon) 1
Entner-Doudoroff Pathway 1
Glycerolipid and Glycerophospholipid Metabolism in Bacteria 1
Ketoisovalerate oxidoreductase 1
Methylglyoxal Metabolism 1
Pyruvate metabolism I: anaplerotic reactions, PEP 1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway #Steps #Present #Specific
L-histidine degradation II 5 5 5
L-histidine degradation I 4 4 4
acetate conversion to acetyl-CoA 1 1 1
acetate and ATP formation from acetyl-CoA III 1 1 1
ethanol degradation II 3 3 2
ethanol degradation IV 3 3 2
ethanol degradation III 3 2 2
L-histidine degradation III 6 4 3
ethylene glycol degradation 2 1 1
putrescine degradation I 2 1 1
putrescine degradation V 2 1 1
L-histidine degradation VI 8 7 3
superpathway of acetate utilization and formation 3 3 1
hypotaurine degradation 3 2 1
putrescine degradation IV 3 2 1
L-isoleucine biosynthesis V 3 2 1
histamine degradation 3 1 1
C4 photosynthetic carbon assimilation cycle, NADP-ME type 7 4 2
chitin deacetylation 4 3 1
phytol degradation 4 3 1
fatty acid α-oxidation I (plants) 4 2 1
L-tryptophan degradation X (mammalian, via tryptamine) 4 2 1
D-arabinose degradation II 4 2 1
putrescine degradation III 4 1 1
mitochondrial NADPH production (yeast) 5 3 1
sphingosine and sphingosine-1-phosphate metabolism 10 4 2
octane oxidation 5 2 1
dopamine degradation 5 1 1
2-methylcitrate cycle I 5 1 1
β-alanine biosynthesis II 6 5 1
methylgallate degradation 6 4 1
L-isoleucine biosynthesis IV 6 4 1
3-methyl-branched fatty acid α-oxidation 6 3 1
superpathway of bitter acids biosynthesis 18 3 3
adlupulone and adhumulone biosynthesis 6 1 1
alkane oxidation 6 1 1
colupulone and cohumulone biosynthesis 6 1 1
2-methylcitrate cycle II 6 1 1
lupulone and humulone biosynthesis 6 1 1
noradrenaline and adrenaline degradation 13 4 2
superpathway of glycol metabolism and degradation 7 5 1
C4 photosynthetic carbon assimilation cycle, PEPCK type 14 8 2
serotonin degradation 7 3 1
ceramide degradation by α-oxidation 7 2 1
limonene degradation IV (anaerobic) 7 1 1
superpathway of NAD/NADP - NADH/NADPH interconversion (yeast) 8 6 1
superpathway of ornithine degradation 8 5 1
protocatechuate degradation I (meta-cleavage pathway) 8 5 1
ceramide and sphingolipid recycling and degradation (yeast) 16 4 2
aromatic biogenic amine degradation (bacteria) 8 1 1
reductive glycine pathway of autotrophic CO2 fixation 9 6 1
Entner-Doudoroff pathway II (non-phosphorylative) 9 5 1
cis-geranyl-CoA degradation 9 2 1
superpathway of coenzyme A biosynthesis II (plants) 10 9 1
superpathway of vanillin and vanillate degradation 10 5 1
superpathway of L-arginine, putrescine, and 4-aminobutanoate degradation 11 7 1
syringate degradation 12 5 1
gluconeogenesis I 13 12 1
superpathway of L-arginine and L-ornithine degradation 13 9 1
superpathway of pentose and pentitol degradation 42 21 1