Culturing: BFirm_ML3, 24 deep-well microplate; Multitron, Aerobic, at 30 (C), shaken=750 rpm
| Pathway | #Steps | #Present | #Specific |
|---|
| benzoyl-CoA biosynthesis | 3 | 3 | 3 |
| fatty acid β-oxidation III (unsaturated, odd number) | 1 | 1 | 1 |
| 3-(4-hydroxyphenyl)pyruvate biosynthesis | 1 | 1 | 1 |
| fatty acid β-oxidation I (generic) | 7 | 5 | 5 |
| oleate β-oxidation | 35 | 29 | 24 |
| adipate degradation | 5 | 5 | 3 |
| adipate biosynthesis | 5 | 4 | 3 |
| glutaryl-CoA degradation | 5 | 3 | 3 |
| fatty acid β-oxidation IV (unsaturated, even number) | 5 | 3 | 3 |
| fatty acid β-oxidation II (plant peroxisome) | 5 | 3 | 3 |
| 2-methyl-branched fatty acid β-oxidation | 14 | 9 | 8 |
| phenylacetate degradation I (aerobic) | 9 | 9 | 5 |
| valproate β-oxidation | 9 | 6 | 5 |
| pyruvate fermentation to hexanol (engineered) | 11 | 7 | 6 |
| acetoacetate degradation (to acetyl CoA) | 2 | 2 | 1 |
| L-phenylalanine biosynthesis III (cytosolic, plants) | 2 | 2 | 1 |
| 3-oxoadipate degradation | 2 | 2 | 1 |
| fatty acid salvage | 6 | 5 | 3 |
| pyruvate fermentation to butanol II (engineered) | 6 | 4 | 3 |
| L-isoleucine degradation I | 6 | 4 | 3 |
| propanoate fermentation to 2-methylbutanoate | 6 | 4 | 3 |
| oleate β-oxidation (thioesterase-dependent, yeast) | 2 | 1 | 1 |
| atromentin biosynthesis | 2 | 1 | 1 |
| L-tyrosine degradation II | 2 | 1 | 1 |
| superpathway of phenylethylamine degradation | 11 | 11 | 5 |
| (8E,10E)-dodeca-8,10-dienol biosynthesis | 11 | 6 | 5 |
| benzoyl-CoA degradation I (aerobic) | 7 | 6 | 3 |
| pyruvate fermentation to butanoate | 7 | 3 | 3 |
| fatty acid β-oxidation VI (mammalian peroxisome) | 7 | 3 | 3 |
| 5,6-dehydrokavain biosynthesis (engineered) | 10 | 6 | 4 |
| (R)- and (S)-3-hydroxybutanoate biosynthesis (engineered) | 5 | 3 | 2 |
| 4-hydroxybenzoate biosynthesis III (plants) | 5 | 3 | 2 |
| 9-cis, 11-trans-octadecadienoyl-CoA degradation (isomerase-dependent, yeast) | 10 | 4 | 4 |
| fatty acid β-oxidation V (unsaturated, odd number, di-isomerase-dependent) | 5 | 2 | 2 |
| pyruvate fermentation to butanol I | 8 | 4 | 3 |
| ketolysis | 3 | 3 | 1 |
| L-tyrosine biosynthesis I | 3 | 3 | 1 |
| ethanol degradation II | 3 | 3 | 1 |
| polyhydroxybutanoate biosynthesis | 3 | 3 | 1 |
| ethanol degradation IV | 3 | 3 | 1 |
| L-phenylalanine biosynthesis I | 3 | 3 | 1 |
| hypotaurine degradation | 3 | 2 | 1 |
| ethanol degradation III | 3 | 2 | 1 |
| L-phenylalanine degradation II (anaerobic) | 3 | 2 | 1 |
| benzoate biosynthesis I (CoA-dependent, β-oxidative) | 9 | 5 | 3 |
| superpathway of Clostridium acetobutylicum acidogenic fermentation | 9 | 5 | 3 |
| methyl ketone biosynthesis (engineered) | 6 | 3 | 2 |
| histamine degradation | 3 | 1 | 1 |
| L-tyrosine degradation IV (to 4-methylphenol) | 3 | 1 | 1 |
| oleate β-oxidation (reductase-dependent, yeast) | 3 | 1 | 1 |
| 3-phenylpropanoate degradation | 10 | 5 | 3 |
| L-glutamate degradation V (via hydroxyglutarate) | 10 | 5 | 3 |
| phytol degradation | 4 | 3 | 1 |
| L-phenylalanine biosynthesis II | 4 | 3 | 1 |
| L-tyrosine biosynthesis III | 4 | 3 | 1 |
| (2S)-ethylmalonyl-CoA biosynthesis | 4 | 3 | 1 |
| L-valine degradation I | 8 | 5 | 2 |
| L-phenylalanine degradation III | 4 | 2 | 1 |
| putrescine degradation III | 4 | 2 | 1 |
| L-tyrosine biosynthesis II | 4 | 2 | 1 |
| fatty acid α-oxidation I (plants) | 4 | 2 | 1 |
| L-tryptophan degradation X (mammalian, via tryptamine) | 4 | 2 | 1 |
| L-tyrosine degradation III | 4 | 2 | 1 |
| L-glutamate degradation VII (to butanoate) | 12 | 3 | 3 |
| 2-methylpropene degradation | 8 | 2 | 2 |
| oleate β-oxidation (isomerase-dependent, yeast) | 4 | 1 | 1 |
| superpathway of Clostridium acetobutylicum solventogenic fermentation | 13 | 8 | 3 |
| superpathway of glyoxylate cycle and fatty acid degradation | 14 | 11 | 3 |
| L-tyrosine degradation I | 5 | 5 | 1 |
| ketogenesis | 5 | 4 | 1 |
| L-tryptophan degradation III (eukaryotic) | 15 | 10 | 3 |
| pyruvate fermentation to acetone | 5 | 3 | 1 |
| mitochondrial NADPH production (yeast) | 5 | 3 | 1 |
| octane oxidation | 5 | 3 | 1 |
| superpathway of L-phenylalanine and L-tyrosine biosynthesis | 5 | 3 | 1 |
| sphingosine and sphingosine-1-phosphate metabolism | 10 | 4 | 2 |
| dopamine degradation | 5 | 2 | 1 |
| isopropanol biosynthesis (engineered) | 5 | 2 | 1 |
| superpathway of plastoquinol biosynthesis | 5 | 2 | 1 |
| methyl tert-butyl ether degradation | 10 | 3 | 2 |
| androstenedione degradation I (aerobic) | 25 | 6 | 5 |
| fatty acid β-oxidation VII (yeast peroxisome) | 5 | 1 | 1 |
| benzoate biosynthesis III (CoA-dependent, non-β-oxidative) | 5 | 1 | 1 |
| 4-hydroxybenzoate biosynthesis I (eukaryotes) | 5 | 1 | 1 |
| L-phenylalanine degradation VI (reductive Stickland reaction) | 5 | 1 | 1 |
| ethylbenzene degradation (anaerobic) | 5 | 1 | 1 |
| L-tyrosine degradation V (reductive Stickland reaction) | 5 | 1 | 1 |
| glycerol degradation to butanol | 16 | 11 | 3 |
| crotonate fermentation (to acetate and cyclohexane carboxylate) | 16 | 3 | 3 |
| superpathway of testosterone and androsterone degradation | 28 | 6 | 5 |
| superpathway of Clostridium acetobutylicum acidogenic and solventogenic fermentation | 17 | 10 | 3 |
| benzoate fermentation (to acetate and cyclohexane carboxylate) | 17 | 4 | 3 |
| catechol degradation III (ortho-cleavage pathway) | 6 | 6 | 1 |
| 3-hydroxypropanoate/4-hydroxybutanate cycle | 18 | 9 | 3 |
| 3-methyl-branched fatty acid α-oxidation | 6 | 3 | 1 |
| 6-gingerol analog biosynthesis (engineered) | 6 | 2 | 1 |
| superpathway of cholesterol degradation I (cholesterol oxidase) | 42 | 9 | 7 |
| toluene degradation VI (anaerobic) | 18 | 3 | 3 |
| 10-cis-heptadecenoyl-CoA degradation (yeast) | 12 | 2 | 2 |
| 10-trans-heptadecenoyl-CoA degradation (reductase-dependent, yeast) | 12 | 2 | 2 |
| 4-ethylphenol degradation (anaerobic) | 6 | 1 | 1 |
| alkane oxidation | 6 | 1 | 1 |
| 10-trans-heptadecenoyl-CoA degradation (MFE-dependent, yeast) | 6 | 1 | 1 |
| jasmonic acid biosynthesis | 19 | 5 | 3 |
| noradrenaline and adrenaline degradation | 13 | 4 | 2 |
| (4Z,7Z,10Z,13Z,16Z)-docosapentaenoate biosynthesis (6-desaturase) | 13 | 2 | 2 |
| superpathway of cholesterol degradation II (cholesterol dehydrogenase) | 47 | 9 | 7 |
| androstenedione degradation II (anaerobic) | 27 | 4 | 4 |
| superpathway of salicylate degradation | 7 | 7 | 1 |
| acetyl-CoA fermentation to butanoate | 7 | 6 | 1 |
| 4-methylcatechol degradation (ortho cleavage) | 7 | 4 | 1 |
| serotonin degradation | 7 | 3 | 1 |
| ceramide degradation by α-oxidation | 7 | 2 | 1 |
| docosahexaenoate biosynthesis III (6-desaturase, mammals) | 14 | 2 | 2 |
| mevalonate pathway I (eukaryotes and bacteria) | 7 | 1 | 1 |
| mevalonate pathway II (haloarchaea) | 7 | 1 | 1 |
| limonene degradation IV (anaerobic) | 7 | 1 | 1 |
| Spodoptera littoralis pheromone biosynthesis | 22 | 4 | 3 |
| superpathway of NAD/NADP - NADH/NADPH interconversion (yeast) | 8 | 6 | 1 |
| 2-deoxy-D-ribose degradation II | 8 | 5 | 1 |
| aromatic biogenic amine degradation (bacteria) | 8 | 4 | 1 |
| ceramide and sphingolipid recycling and degradation (yeast) | 16 | 4 | 2 |
| mevalonate pathway IV (archaea) | 8 | 1 | 1 |
| mevalonate pathway III (Thermoplasma) | 8 | 1 | 1 |
| isoprene biosynthesis II (engineered) | 8 | 1 | 1 |
| cholesterol degradation to androstenedione I (cholesterol oxidase) | 17 | 3 | 2 |
| platensimycin biosynthesis | 26 | 6 | 3 |
| superpathway of aromatic amino acid biosynthesis | 18 | 18 | 2 |
| aromatic compounds degradation via β-ketoadipate | 9 | 9 | 1 |
| 1-butanol autotrophic biosynthesis (engineered) | 27 | 19 | 3 |
| L-phenylalanine degradation IV (mammalian, via side chain) | 9 | 6 | 1 |
| benzoyl-CoA degradation III (anaerobic) | 9 | 3 | 1 |
| 4-oxopentanoate degradation | 9 | 2 | 1 |
| superpathway of L-tyrosine biosynthesis | 10 | 10 | 1 |
| L-histidine biosynthesis | 10 | 10 | 1 |
| superpathway of L-phenylalanine biosynthesis | 10 | 10 | 1 |
| superpathway of geranylgeranyldiphosphate biosynthesis I (via mevalonate) | 10 | 4 | 1 |
| L-lysine fermentation to acetate and butanoate | 10 | 3 | 1 |
| rosmarinic acid biosynthesis I | 10 | 1 | 1 |
| toluene degradation III (aerobic) (via p-cresol) | 11 | 7 | 1 |
| gallate degradation III (anaerobic) | 11 | 5 | 1 |
| ethylmalonyl-CoA pathway | 11 | 3 | 1 |
| (S)-reticuline biosynthesis I | 11 | 3 | 1 |
| cholesterol degradation to androstenedione II (cholesterol dehydrogenase) | 22 | 3 | 2 |
| tropane alkaloids biosynthesis | 11 | 1 | 1 |
| superpathway of cholesterol degradation III (oxidase) | 49 | 5 | 4 |
| photosynthetic 3-hydroxybutanoate biosynthesis (engineered) | 26 | 18 | 2 |
| superpathway of rosmarinic acid biosynthesis | 14 | 2 | 1 |
| superpathway of hyoscyamine (atropine) and scopolamine biosynthesis | 16 | 3 | 1 |
| mandelate degradation to acetyl-CoA | 18 | 12 | 1 |
| sitosterol degradation to androstenedione | 18 | 1 | 1 |
| superpathway of ergosterol biosynthesis I | 26 | 5 | 1 |
| anaerobic aromatic compound degradation (Thauera aromatica) | 27 | 5 | 1 |
| superpathway of chorismate metabolism | 59 | 42 | 2 |
| superpathway of aerobic toluene degradation | 30 | 14 | 1 |
| superpathway of aromatic compound degradation via 3-oxoadipate | 35 | 21 | 1 |
| superpathway of cholesterol biosynthesis | 38 | 5 | 1 |
| superpathway of L-lysine degradation | 43 | 11 | 1 |
| superpathway of histidine, purine, and pyrimidine biosynthesis | 46 | 44 | 1 |
| Methanobacterium thermoautotrophicum biosynthetic metabolism | 56 | 21 | 1 |