Experiment set1IT023 for Caulobacter crescentus NA1000
a-Cyclodextrin carbon source
Group: carbon sourceMedia: M2_noCarbon + a-Cyclodextrin (20 mM)
Culturing: Caulo_ML2, 24-well transparent microplate; Multitron, Aerobic, at 30 (C), shaken=700 rpm
Growth: about 5.9 generations
By: Jayashree on 22-Mar-17
Media components: 1.74 g/L Disodium phosphate, 1.06 g/L Potassium phosphate monobasic, 0.5 g/L Ammonium chloride, 0.5 mM Magnesium sulfate, 0.5 mM Calcium chloride, 0.01 mM Iron (II) sulfate heptahydrate, 0.008 mM EDTA
Growth plate: 1522 A6
Specific Phenotypes
For 4 genes in this experiment
For carbon source a-Cyclodextrin in Caulobacter crescentus NA1000
For carbon source a-Cyclodextrin across organisms
SEED Subsystems
| Subsystem | #Specific |
|---|---|
| Chorismate: Intermediate for synthesis of PAPA antibiotics, PABA, anthranilate, 3-hydroxyanthranilate and more. | 1 |
| Folate Biosynthesis | 1 |
| Maltose and Maltodextrin Utilization | 1 |
Metabolic Maps
Color code by fitness: see overview map or list of maps.
Maps containing gene(s) with specific phenotypes:
- Galactose metabolism
- Starch and sucrose metabolism
- Folate biosynthesis
- Biosynthesis of phenylpropanoids
MetaCyc Pathways
Pathways that contain genes with specific phenotypes:
| Pathway | #Steps | #Present | #Specific |
|---|---|---|---|
| L-glutamine degradation I | 1 | 1 | 1 |
| L-glutamate biosynthesis I | 2 | 2 | 1 |
| 4-aminobenzoate biosynthesis I | 2 | 2 | 1 |
| ammonia assimilation cycle III | 3 | 3 | 1 |
| starch degradation I | 3 | 1 | 1 |
| L-asparagine biosynthesis III (tRNA-dependent) | 4 | 4 | 1 |
| glutaminyl-tRNAgln biosynthesis via transamidation | 4 | 4 | 1 |
| L-glutamate and L-glutamine biosynthesis | 7 | 4 | 1 |
| L-citrulline biosynthesis | 8 | 5 | 1 |
| glycogen degradation I | 8 | 3 | 1 |
| chloramphenicol biosynthesis | 9 | 1 | 1 |
| superpathway of tetrahydrofolate biosynthesis | 10 | 8 | 1 |
| superpathway of candicidin biosynthesis | 11 | 4 | 1 |
| superpathway of tetrahydrofolate biosynthesis and salvage | 12 | 10 | 1 |
| superpathway of L-citrulline metabolism | 12 | 7 | 1 |
| superpathway of chorismate metabolism | 59 | 38 | 1 |