Experiment set1IT015 for Caulobacter crescentus NA1000
D-Salicin carbon source
Group: carbon sourceMedia: M2_noCarbon + D-Salicin (20 mM)
Culturing: Caulo_ML2, 24-well transparent microplate; Multitron, Aerobic, at 30 (C), shaken=700 rpm
Growth: about 4.4 generations
By: Jayashree on 22-Mar-17
Media components: 1.74 g/L Disodium phosphate, 1.06 g/L Potassium phosphate monobasic, 0.5 g/L Ammonium chloride, 0.5 mM Magnesium sulfate, 0.5 mM Calcium chloride, 0.01 mM Iron (II) sulfate heptahydrate, 0.008 mM EDTA
Growth plate: 1521 C5
Specific Phenotypes
For 3 genes in this experiment
For carbon source D-Salicin in Caulobacter crescentus NA1000
For carbon source D-Salicin across organisms
SEED Subsystems
| Subsystem | #Specific |
|---|---|
| Beta-Glucoside Metabolism | 1 |
| Fructooligosaccharides(FOS) and Raffinose Utilization | 1 |
| Respiratory dehydrogenases 1 | 1 |
| Xylose utilization | 1 |
Metabolic Maps
Color code by fitness: see overview map or list of maps.
Maps containing gene(s) with specific phenotypes:
- Starch and sucrose metabolism
- Pentose and glucuronate interconversions
- Cyanoamino acid metabolism
- Phenylpropanoid biosynthesis
MetaCyc Pathways
Pathways that contain genes with specific phenotypes:
| Pathway | #Steps | #Present | #Specific |
|---|---|---|---|
| neolinustatin bioactivation | 3 | 2 | 2 |
| linustatin bioactivation | 4 | 2 | 2 |
| lotaustralin degradation | 2 | 1 | 1 |
| linamarin degradation | 2 | 1 | 1 |
| lactose degradation II | 3 | 2 | 1 |
| cellulose degradation II (fungi) | 3 | 1 | 1 |
| sucrose degradation VII (sucrose 3-dehydrogenase) | 4 | 2 | 1 |
| D-xylose degradation V | 5 | 5 | 1 |
| coumarin biosynthesis (via 2-coumarate) | 5 | 2 | 1 |
| α-tomatine degradation | 6 | 1 | 1 |
| firefly bioluminescence | 14 | 2 | 1 |