Experiment set1IT013 for Pseudomonas fluorescens FW300-N2C3

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Potassium acetate carbon source

Group: carbon source
Media: RCH2_defined_noCarbon + Potassium acetate (20 mM), pH=7.2
Culturing: pseudo5_N2-C3_1_ML2, tube, Aerobic, at 30 (C), shaken=200 rpm
Growth: about 3.7 generations
By: Jayashree on 4/28/2014
Media components: 0.25 g/L Ammonium chloride, 0.1 g/L Potassium Chloride, 0.6 g/L Sodium phosphate monobasic monohydrate, 30 mM PIPES sesquisodium salt, Wolfe's mineral mix (0.03 g/L Magnesium Sulfate Heptahydrate, 0.015 g/L Nitrilotriacetic acid, 0.01 g/L Sodium Chloride, 0.005 g/L Manganese (II) sulfate monohydrate, 0.001 g/L Cobalt chloride hexahydrate, 0.001 g/L Zinc sulfate heptahydrate, 0.001 g/L Calcium chloride dihydrate, 0.001 g/L Iron (II) sulfate heptahydrate, 0.00025 g/L Nickel (II) chloride hexahydrate, 0.0002 g/L Aluminum potassium sulfate dodecahydrate, 0.0001 g/L Copper (II) sulfate pentahydrate, 0.0001 g/L Boric Acid, 0.0001 g/L Sodium Molybdate Dihydrate, 0.003 mg/L Sodium selenite pentahydrate), Wolfe's vitamin mix (0.1 mg/L Pyridoxine HCl, 0.05 mg/L 4-Aminobenzoic acid, 0.05 mg/L Lipoic acid, 0.05 mg/L Nicotinic Acid, 0.05 mg/L Riboflavin, 0.05 mg/L Thiamine HCl, 0.05 mg/L calcium pantothenate, 0.02 mg/L biotin, 0.02 mg/L Folic Acid, 0.001 mg/L Cyanocobalamin)

Specific Phenotypes

For 21 genes in this experiment

For carbon source Potassium acetate in Pseudomonas fluorescens FW300-N2C3

For carbon source Potassium acetate across organisms

SEED Subsystems

Subsystem #Specific
Multidrug Resistance, Tripartite Systems Found in Gram Negative Bacteria 2
Carboxysome 1
Cyanate hydrolysis 1
D-galactonate catabolism 1
Ketoisovalerate oxidoreductase 1
Methionine Salvage 1
NAD and NADP cofactor biosynthesis global 1
Phosphate metabolism 1
Pyruvate metabolism II: acetyl-CoA, acetogenesis from pyruvate 1
RNA processing and degradation, bacterial 1
Terminal cytochrome C oxidases 1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway #Steps #Present #Specific
3-methylthiopropanoate biosynthesis 1 1 1
acetate and ATP formation from acetyl-CoA III 1 1 1
acetate conversion to acetyl-CoA 1 1 1
CO2 fixation into oxaloacetate (anaplerotic) 2 2 1
arsenite to oxygen electron transfer 2 1 1
ethanol degradation II 3 3 1
superpathway of acetate utilization and formation 3 3 1
D-galactonate degradation 3 3 1
ethanol degradation IV 3 3 1
cyanate degradation 3 3 1
L-isoleucine biosynthesis V 3 2 1
ethanol degradation III 3 2 1
arsenite to oxygen electron transfer (via azurin) 3 1 1
aerobic respiration I (cytochrome c) 4 3 1
aerobic respiration II (cytochrome c) (yeast) 4 3 1
chitin deacetylation 4 2 1
2-methylcitrate cycle I 5 5 1
2-methylcitrate cycle II 6 5 1
β-alanine biosynthesis II 6 5 1
L-isoleucine biosynthesis IV 6 4 1
Fe(II) oxidation 6 3 1
superpathway of bitter acids biosynthesis 18 3 3
lupulone and humulone biosynthesis 6 1 1
adlupulone and adhumulone biosynthesis 6 1 1
colupulone and cohumulone biosynthesis 6 1 1
C4 photosynthetic carbon assimilation cycle, NADP-ME type 7 4 1
L-glucose degradation 7 3 1
S-methyl-5-thio-α-D-ribose 1-phosphate degradation I 7 2 1
reductive glycine pathway of autotrophic CO2 fixation 9 5 1
cis-geranyl-CoA degradation 9 3 1
superpathway of coenzyme A biosynthesis II (plants) 10 9 1
peptidoglycan recycling II 10 8 1
C4 photosynthetic carbon assimilation cycle, NAD-ME type 11 6 1
L-methionine salvage cycle III 11 5 1
L-methionine salvage cycle II (plants) 11 3 1
L-methionine salvage cycle I (bacteria and plants) 12 4 1
3-hydroxypropanoate cycle 13 5 1
glyoxylate assimilation 13 4 1
peptidoglycan recycling I 14 11 1
C4 photosynthetic carbon assimilation cycle, PEPCK type 14 9 1
gluconeogenesis II (Methanobacterium thermoautotrophicum) 18 9 1
3-hydroxypropanoate/4-hydroxybutanate cycle 18 8 1
superpathway of the 3-hydroxypropanoate cycle 18 5 1
Methanobacterium thermoautotrophicum biosynthetic metabolism 56 21 1