Experiment set1IT012 for Pseudomonas fluorescens FW300-N2C3
D-Gluconic Acid sodium salt carbon source
Group: carbon sourceMedia: RCH2_defined_noCarbon + D-Gluconic Acid sodium salt (20 mM), pH=7.2
Culturing: pseudo5_N2-C3_1_ML2, tube, Aerobic, at 30 (C), shaken=200 rpm
Growth: about 5.8 generations
By: Jayashree on 4/28/2014
Media components: 0.25 g/L Ammonium chloride, 0.1 g/L Potassium Chloride, 0.6 g/L Sodium phosphate monobasic monohydrate, 30 mM PIPES sesquisodium salt, Wolfe's mineral mix (0.03 g/L Magnesium Sulfate Heptahydrate, 0.015 g/L Nitrilotriacetic acid, 0.01 g/L Sodium Chloride, 0.005 g/L Manganese (II) sulfate monohydrate, 0.001 g/L Cobalt chloride hexahydrate, 0.001 g/L Zinc sulfate heptahydrate, 0.001 g/L Calcium chloride dihydrate, 0.001 g/L Iron (II) sulfate heptahydrate, 0.00025 g/L Nickel (II) chloride hexahydrate, 0.0002 g/L Aluminum potassium sulfate dodecahydrate, 0.0001 g/L Copper (II) sulfate pentahydrate, 0.0001 g/L Boric Acid, 0.0001 g/L Sodium Molybdate Dihydrate, 0.003 mg/L Sodium selenite pentahydrate), Wolfe's vitamin mix (0.1 mg/L Pyridoxine HCl, 0.05 mg/L 4-Aminobenzoic acid, 0.05 mg/L Lipoic acid, 0.05 mg/L Nicotinic Acid, 0.05 mg/L Riboflavin, 0.05 mg/L Thiamine HCl, 0.05 mg/L calcium pantothenate, 0.02 mg/L biotin, 0.02 mg/L Folic Acid, 0.001 mg/L Cyanocobalamin)
Specific Phenotypes
For 1 genes in this experiment
For carbon source D-Gluconic Acid sodium salt in Pseudomonas fluorescens FW300-N2C3
For carbon source D-Gluconic Acid sodium salt across organisms
SEED Subsystems
Metabolic Maps
Color code by fitness: see overview map or list of maps.
Maps containing gene(s) with specific phenotypes:
- Glycine, serine and threonine metabolism
- Glycerolipid metabolism
- Glyoxylate and dicarboxylate metabolism
MetaCyc Pathways
Pathways that contain genes with specific phenotypes:
| Pathway | #Steps | #Present | #Specific |
|---|---|---|---|
| 2-O-α-mannosyl-D-glycerate degradation | 2 | 1 | 1 |
| glycolate and glyoxylate degradation I | 4 | 4 | 1 |
| D-glucarate degradation I | 4 | 3 | 1 |
| D-galactarate degradation I | 4 | 3 | 1 |
| superpathway of D-glucarate and D-galactarate degradation | 5 | 4 | 1 |
| superpathway of glycol metabolism and degradation | 7 | 7 | 1 |
| 2-deoxy-D-ribose degradation II | 8 | 4 | 1 |
| Entner-Doudoroff pathway II (non-phosphorylative) | 9 | 7 | 1 |
| photorespiration I | 9 | 5 | 1 |
| photorespiration III | 9 | 5 | 1 |
| photorespiration II | 10 | 6 | 1 |
| formaldehyde assimilation I (serine pathway) | 13 | 6 | 1 |
| superpathway of microbial D-galacturonate and D-glucuronate degradation | 31 | 17 | 1 |