Experiment set1IT005 for Sinorhizobium meliloti 1021

D-Arabinose carbon source

Group: carbon source
Media: RCH2_defined_noCarbon + D-Arabinose (20 mM), pH=7
Culturing: Smeli_ML6, 24 deep-well microplate; Multitron, Aerobic, at 30 (C), shaken=750 rpm
Growth: about 4.2 generations
By: Mark on 6/2/2015
Media components: 0.25 g/L Ammonium chloride, 0.1 g/L Potassium Chloride, 0.6 g/L Sodium phosphate monobasic monohydrate, 30 mM PIPES sesquisodium salt, Wolfe's mineral mix (0.03 g/L Magnesium Sulfate Heptahydrate, 0.015 g/L Nitrilotriacetic acid, 0.01 g/L Sodium Chloride, 0.005 g/L Manganese (II) sulfate monohydrate, 0.001 g/L Cobalt chloride hexahydrate, 0.001 g/L Zinc sulfate heptahydrate, 0.001 g/L Calcium chloride dihydrate, 0.001 g/L Iron (II) sulfate heptahydrate, 0.00025 g/L Nickel (II) chloride hexahydrate, 0.0002 g/L Aluminum potassium sulfate dodecahydrate, 0.0001 g/L Copper (II) sulfate pentahydrate, 0.0001 g/L Boric Acid, 0.0001 g/L Sodium Molybdate Dihydrate, 0.003 mg/L Sodium selenite pentahydrate), Wolfe's vitamin mix (0.1 mg/L Pyridoxine HCl, 0.05 mg/L 4-Aminobenzoic acid, 0.05 mg/L Lipoic acid, 0.05 mg/L Nicotinic Acid, 0.05 mg/L Riboflavin, 0.05 mg/L Thiamine HCl, 0.05 mg/L calcium pantothenate, 0.02 mg/L biotin, 0.02 mg/L Folic Acid, 0.001 mg/L Cyanocobalamin)
Growth plate: Smeli_carbon_plate1 A5

Specific Phenotypes

For 11 genes in this experiment

For carbon source D-Arabinose in Sinorhizobium meliloti 1021

For carbon source D-Arabinose across organisms

SEED Subsystems

Subsystem	#Specific
L-fucose utilization temp	2
CO2 uptake, carboxysome	1
Carboxysome	1
Chitin and N-acetylglucosamine utilization	1
Fructose utilization	1
Gentisare degradation	1
Ribitol, Xylitol, Arabitol, Mannitol and Sorbitol utilization	1
Salicylate and gentisate catabolism	1
Xylose utilization	1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

• Pentose and glucuronate interconversions
• Ascorbate and aldarate metabolism

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway	#Steps	#Present	#Specific
xylitol degradation I	2	2	1
D-xylose degradation I	2	2	1
D-arabinitol degradation I	2	1	1
D-arabinose degradation IV	6	4	2
D-galacturonate degradation I	5	4	1
D-xylose degradation VI	5	3	1
L-arabinose degradation V	6	4	1
D-arabinose degradation III	6	3	1
L-rhamnose degradation III	7	3	1
L-fucose degradation III	8	5	1
superpathway of hexuronide and hexuronate degradation	10	8	1
superpathway of glucose and xylose degradation	17	15	1
superpathway of pentose and pentitol degradation	42	21	2
superpathway of microbial D-galacturonate and D-glucuronate degradation	31	19	1