Experiment set1IT005 for Phocaeicola vulgatus CL09T03C04

Pectic galactan from potato 5mg/ml carbon source

Group: carbon source
Media: Varel_Bryant_medium + Pectic galactan from potato (5 mg/ml)
Culturing: Bvulgatus_CL09T03C04_ML5, 96 deep-well microplate; 1.2 mL volume, Anaerobic, at 34 (C), shaken=0 rpm
By: Surya Tripathi on 12/22/21
Media components: 15 uM Hemin, 134 uM L-Methionine, 15 uM Iron (II) sulfate heptahydrate, 8.25 mM L-Cysteine, 23.8 mM Sodium bicarbonate, Mineral 3B solution (6.6 mM Potassium phosphate monobasic, 15.4 mM Sodium Chloride, 98 uM Magnesium chloride hexahydrate, 176.5 uM Calcium chloride dihydrate, 4.2 uM Cobalt chloride hexahydrate, 50.5 uM Manganese (II) chloride tetrahydrate, 9.3 mM Ammonium chloride, 1.75 mM Sodium sulfate)

Specific Phenotypes

For 13 genes in this experiment

For carbon source Pectic galactan from potato in Phocaeicola vulgatus CL09T03C04

For carbon source Pectic galactan from potato across organisms

SEED Subsystems

Subsystem	#Specific
LOS core oligosaccharide biosynthesis	2
Cobalt-zinc-cadmium resistance	1
Galactosylceramide and Sulfatide metabolism	1
Lactose and Galactose Uptake and Utilization	1
Lactose utilization	1
Ribitol, Xylitol, Arabitol, Mannitol and Sorbitol utilization	1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

• Sphingolipid metabolism
• Fructose and mannose metabolism
• Galactose metabolism
• Aminophosphonate metabolism
• Other glycan degradation
• High-mannose type N-glycan biosynthesis
• Nucleotide sugars metabolism
• Glycosaminoglycan degradation
• Glycerophospholipid metabolism
• Glycosphingolipid biosynthesis - ganglio series

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway	#Steps	#Present	#Specific
L-galactonate degradation	1	1	1
lactose degradation III	1	1	1
xyloglucan degradation II (exoglucanase)	8	5	3
D-sorbitol degradation I	3	3	1