Experiment set1H46 for Marinobacter adhaerens HP15
Defined media with L-Phenylalanine as nitrogen source
Group: nitrogen sourceMedia: DinoMM_alphaKetoGlutAcid_NoNitrogen + L-Phenylalanine (1 mM)
Culturing: Marino_ML2, 48 well microplate; Tecan Infinite F200, Aerobic, at 30 (C), shaken=orbital
By: Jordan on 9/5/2013
Media components: 20 g/L Sea salts, 0.1 g/L Potassium phosphate monobasic, 10 mM a-Ketoglutaric acid disodium salt hydrate, Wolfe's mineral mix (0.003 g/L Magnesium Sulfate Heptahydrate, 0.0015 g/L Nitrilotriacetic acid, 0.001 g/L Sodium Chloride, 0.0005 g/L Manganese (II) sulfate monohydrate, 0.0001 g/L Cobalt chloride hexahydrate, 0.0001 g/L Zinc sulfate heptahydrate, 0.0001 g/L Calcium chloride dihydrate, 0.0001 g/L Iron (II) sulfate heptahydrate, 2.5e-05 g/L Nickel (II) chloride hexahydrate, 2e-05 g/L Aluminum potassium sulfate dodecahydrate, 1e-05 g/L Copper (II) sulfate pentahydrate, 1e-05 g/L Boric Acid, 1e-05 g/L Sodium Molybdate Dihydrate, 0.0003 mg/L Sodium selenite pentahydrate), Wolfe's vitamin mix (0.05 mg/L Pyridoxine HCl, 0.025 mg/L 4-Aminobenzoic acid, 0.025 mg/L Lipoic acid, 0.025 mg/L Nicotinic Acid, 0.025 mg/L Riboflavin, 0.025 mg/L Thiamine HCl, 0.025 mg/L calcium pantothenate, 0.01 mg/L biotin, 0.01 mg/L Folic Acid, 0.0005 mg/L Cyanocobalamin)
Growth plate: 677 C5,C6
Specific Phenotypes
For 5 genes in this experiment
For nitrogen source L-Phenylalanine in Marinobacter adhaerens HP15
For nitrogen source L-Phenylalanine across organisms
SEED Subsystems
Metabolic Maps
Color code by fitness: see overview map or list of maps.
Maps containing gene(s) with specific phenotypes:
- Fatty acid metabolism
- Valine, leucine and isoleucine degradation
- Lysine degradation
- Benzoate degradation via CoA ligation
- Butanoate metabolism
- Biosynthesis of unsaturated fatty acids
- Fatty acid elongation in mitochondria
- Geraniol degradation
- Benzoate degradation via hydroxylation
- Tryptophan metabolism
- alpha-Linolenic acid metabolism
- Propanoate metabolism
- Ethylbenzene degradation
- Limonene and pinene degradation
- Biosynthesis of plant hormones
- Fatty acid biosynthesis
- Synthesis and degradation of ketone bodies
- Histidine metabolism
- Tyrosine metabolism
- Phenylalanine metabolism
- beta-Alanine metabolism
- Lipopolysaccharide biosynthesis
- Glycerophospholipid metabolism
- Ether lipid metabolism
- Glycosphingolipid biosynthesis - ganglio series
- Pyruvate metabolism
- 1- and 2-Methylnaphthalene degradation
- Terpenoid biosynthesis
- Diterpenoid biosynthesis
- Carotenoid biosynthesis - General
- Caprolactam degradation
- Anthocyanin biosynthesis
- Alkaloid biosynthesis I
- Alkaloid biosynthesis II
- Biosynthesis of type II polyketide backbone
- Biosynthesis of terpenoids and steroids
MetaCyc Pathways
Pathways that contain genes with specific phenotypes: