Experiment set1H4 for Shewanella amazonensis SB2B

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Minimal media with D-Maltose monohydrate as carbon source

Group: carbon source
Media: ShewMM_noCarbon + D-Maltose monohydrate (20 mM), pH=7
Culturing: SB2B_ML5, 48 well microplate; Tecan Infinite F200, Aerobic, at 37 (C), shaken=orbital
By: Jake on 4/15/2013
Media components: 1.5 g/L Ammonium chloride, 1.75 g/L Sodium Chloride, 0.61 g/L Magnesium chloride hexahydrate, 0.1 g/L Potassium Chloride, 0.6 g/L Sodium phosphate monobasic monohydrate, 30 mM PIPES sesquisodium salt, Wolfe's mineral mix (0.03 g/L Magnesium Sulfate Heptahydrate, 0.015 g/L Nitrilotriacetic acid, 0.01 g/L Sodium Chloride, 0.005 g/L Manganese (II) sulfate monohydrate, 0.001 g/L Cobalt chloride hexahydrate, 0.001 g/L Zinc sulfate heptahydrate, 0.001 g/L Calcium chloride dihydrate, 0.001 g/L Iron (II) sulfate heptahydrate, 0.00025 g/L Nickel (II) chloride hexahydrate, 0.0002 g/L Aluminum potassium sulfate dodecahydrate, 0.0001 g/L Copper (II) sulfate pentahydrate, 0.0001 g/L Boric Acid, 0.0001 g/L Sodium Molybdate Dihydrate, 0.003 mg/L Sodium selenite pentahydrate), Wolfe's vitamin mix (0.1 mg/L Pyridoxine HCl, 0.05 mg/L 4-Aminobenzoic acid, 0.05 mg/L Lipoic acid, 0.05 mg/L Nicotinic Acid, 0.05 mg/L Riboflavin, 0.05 mg/L Thiamine HCl, 0.05 mg/L calcium pantothenate, 0.02 mg/L biotin, 0.02 mg/L Folic Acid, 0.001 mg/L Cyanocobalamin)
Growth plate: 509 A3,A4

Specific Phenotypes

For 8 genes in this experiment

For carbon source D-Maltose monohydrate in Shewanella amazonensis SB2B

For carbon source D-Maltose monohydrate across organisms

SEED Subsystems

Subsystem #Specific
Maltose and Maltodextrin Utilization 3
Arginine Deiminase Pathway 1
Arginine and Ornithine Degradation 1
Beta-Glucoside Metabolism 1
Calvin-Benson cycle 1
Lactose and Galactose Uptake and Utilization 1
Mannose-sensitive hemagglutinin type 4 pilus 1
Polyamine Metabolism 1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway #Steps #Present #Specific
sucrose degradation III (sucrose invertase) 4 3 2
trehalose degradation II (cytosolic) 2 1 1
D-mannose degradation II 2 1 1
trehalose degradation VI (periplasmic) 2 1 1
trehalose degradation I (low osmolarity) 2 1 1
trehalose degradation V 3 2 1
GDP-α-D-glucose biosynthesis 3 2 1
D-sorbitol degradation I 3 2 1
sucrose degradation I (sucrose phosphotransferase) 3 1 1
trehalose degradation IV 3 1 1
sucrose degradation IV (sucrose phosphorylase) 4 3 1
mannitol degradation II 4 2 1
CMP-2-keto-3-deoxy-D-glycero-D-galacto-nononate biosynthesis 4 1 1
sucrose degradation VII (sucrose 3-dehydrogenase) 4 1 1
D-galactose degradation I (Leloir pathway) 5 4 1
sucrose degradation II (sucrose synthase) 5 4 1
mannitol cycle 5 3 1
glucose and glucose-1-phosphate degradation 5 3 1
1,5-anhydrofructose degradation 5 2 1
glycogen degradation II 6 5 1
UDP-N-acetyl-D-glucosamine biosynthesis II 6 4 1
UDP-N-acetyl-D-galactosamine biosynthesis II 7 5 1
glycogen degradation I 8 7 1
sucrose biosynthesis II 8 6 1
heterolactic fermentation 18 15 2
chitin biosynthesis 9 5 1
1,3-propanediol biosynthesis (engineered) 9 4 1
Rubisco shunt 10 9 1
glycolysis III (from glucose) 11 9 1
homolactic fermentation 12 9 1
Calvin-Benson-Bassham cycle 13 11 1
Bifidobacterium shunt 15 13 1
superpathway of CMP-sialic acids biosynthesis 15 4 1
oxygenic photosynthesis 17 12 1
superpathway of anaerobic sucrose degradation 19 15 1
ethene biosynthesis V (engineered) 25 19 1
photosynthetic 3-hydroxybutanoate biosynthesis (engineered) 26 21 1
1-butanol autotrophic biosynthesis (engineered) 27 20 1