Experiment set1H39 for Marinobacter adhaerens HP15

Defined media with L-Glutamine as nitrogen source

Group: nitrogen source
Media: DinoMM_alphaKetoGlutAcid_NoNitrogen + L-Glutamine (1 mM)
Culturing: Marino_ML2, 48 well microplate; Tecan Infinite F200, Aerobic, at 30 (C), shaken=orbital
By: Jordan on 9/5/2013
Media components: 20 g/L Sea salts, 0.1 g/L Potassium phosphate monobasic, 10 mM a-Ketoglutaric acid disodium salt hydrate, Wolfe's mineral mix (0.003 g/L Magnesium Sulfate Heptahydrate, 0.0015 g/L Nitrilotriacetic acid, 0.001 g/L Sodium Chloride, 0.0005 g/L Manganese (II) sulfate monohydrate, 0.0001 g/L Cobalt chloride hexahydrate, 0.0001 g/L Zinc sulfate heptahydrate, 0.0001 g/L Calcium chloride dihydrate, 0.0001 g/L Iron (II) sulfate heptahydrate, 2.5e-05 g/L Nickel (II) chloride hexahydrate, 2e-05 g/L Aluminum potassium sulfate dodecahydrate, 1e-05 g/L Copper (II) sulfate pentahydrate, 1e-05 g/L Boric Acid, 1e-05 g/L Sodium Molybdate Dihydrate, 0.0003 mg/L Sodium selenite pentahydrate), Wolfe's vitamin mix (0.05 mg/L Pyridoxine HCl, 0.025 mg/L 4-Aminobenzoic acid, 0.025 mg/L Lipoic acid, 0.025 mg/L Nicotinic Acid, 0.025 mg/L Riboflavin, 0.025 mg/L Thiamine HCl, 0.025 mg/L calcium pantothenate, 0.01 mg/L biotin, 0.01 mg/L Folic Acid, 0.0005 mg/L Cyanocobalamin)
Growth plate: 677 A7,A8

Specific Phenotypes

For 9 genes in this experiment

For nitrogen source L-Glutamine in Marinobacter adhaerens HP15

For nitrogen source L-Glutamine across organisms

SEED Subsystems

Subsystem	#Specific
ABC transporter dipeptide (TC 3.A.1.5.2)	3
Methionine Biosynthesis	2
Methionine Degradation	2
Bacterial Chemotaxis	1
Branched-Chain Amino Acid Biosynthesis	1
Glycolate, glyoxylate interconversions	1
Leucine Degradation and HMG-CoA Metabolism	1
Polyamine Metabolism	1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

• Urea cycle and metabolism of amino groups
• Methionine metabolism
• Valine, leucine and isoleucine degradation
• Valine, leucine and isoleucine biosynthesis
• Arginine and proline metabolism

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway	#Steps	#Present	#Specific
spermidine biosynthesis I	2	2	1
spermine biosynthesis	2	1	1
aminopropylcadaverine biosynthesis	3	2	1
spermidine biosynthesis III	4	2	1
superpathway of polyamine biosynthesis II	8	6	1
superpathway of polyamine biosynthesis I	8	5	1
L-methionine salvage cycle III	11	9	1
L-methionine salvage cycle I (bacteria and plants)	12	8	1
superpathway of arginine and polyamine biosynthesis	17	14	1