Experiment set1H33 for Dinoroseobacter shibae DFL-12
marine broth with MreB Perturbing Compound A22 0.01 mg/ml
Group: stressMedia: marine_broth_2216 + MreB Perturbing Compound A22 (0.01 mg/ml)
Culturing: Dino_ML3, 48 well microplate; Tecan Infinite F200, Aerobic, at 30 (C), shaken=orbital
By: Jordan on 8/16/2013
Media components: 5 g/L Bacto Peptone, 1 g/L Yeast Extract, 0.1 g/L Ferric citrate, 19.45 g/L Sodium Chloride, 5.9 g/L Magnesium chloride hexahydrate, 3.24 g/L Magnesium sulfate, 1.8 g/L Calcium chloride, 0.55 g/L Potassium Chloride, 0.16 g/L Sodium bicarbonate, 0.08 g/L Potassium bromide, 34 mg/L Strontium chloride, 22 mg/L Boric Acid, 4 mg/L Sodium metasilicate, 2.4 mg/L sodium fluoride, 8 mg/L Disodium phosphate
Growth plate: 646 B7,B8
Specific Phenotypes
For 1 genes in this experiment
For stress MreB Perturbing Compound A22 in Dinoroseobacter shibae DFL-12
For stress MreB Perturbing Compound A22 across organisms
SEED Subsystems
| Subsystem | #Specific |
|---|---|
| D-Galacturonate and D-Glucuronate Utilization | 1 |
| Proline, 4-hydroxyproline uptake and utilization | 1 |
Metabolic Maps
Color code by fitness: see overview map or list of maps.
Maps containing gene(s) with specific phenotypes:
MetaCyc Pathways
Pathways that contain genes with specific phenotypes:
| Pathway | #Steps | #Present | #Specific |
|---|---|---|---|
| pyruvate fermentation to acetate VIII | 2 | 1 | 1 |
| L-lyxonate degradation | 3 | 3 | 1 |
| D-glucarate degradation II | 3 | 1 | 1 |
| D-galactarate degradation II | 3 | 1 | 1 |
| L-carnitine degradation II | 3 | 1 | 1 |
| superpathway of NAD/NADP - NADH/NADPH interconversion (yeast) | 8 | 6 | 2 |
| 1,2-dichloroethane degradation | 4 | 3 | 1 |
| trans-4-hydroxy-L-proline degradation II | 4 | 1 | 1 |
| cytosolic NADPH production (yeast) | 5 | 4 | 1 |
| mitochondrial NADPH production (yeast) | 5 | 3 | 1 |
| D-glucuronate degradation II | 5 | 2 | 1 |
| D-galacturonate degradation II | 5 | 1 | 1 |
| D-xylose degradation V | 5 | 1 | 1 |
| D-xylose degradation III | 5 | 1 | 1 |
| L-arabinose degradation III | 6 | 3 | 1 |
| D-arabinose degradation III | 6 | 2 | 1 |
| superpathway of pentose and pentitol degradation | 42 | 15 | 2 |
| superpathway of microbial D-galacturonate and D-glucuronate degradation | 31 | 9 | 1 |