Experiment set1H31 for Desulfovibrio vulgaris Miyazaki F

Compare to:

MoYLS4 with Chloramphenicol 0.003125 mg/ml

Group: stress
Media: MoYLS4 + Chloramphenicol (0.003125 mg/ml), pH=7.2
Culturing: Miya_ML3, hungate, Anaerobic, at 30 (C), shaken=0 rpm
Growth: about 4.7 generations
By: Jen on 3/20/2014
Media components: 1 g/L Yeast Extract, 30 mM Sodium sulfate, 60 mM Sodium D,L-Lactate, 20 mM Ammonium chloride, 30 mM Tris hydrochloride, 0.12 mM EDTA, 1 mM Sodium sulfide nonahydrate, 8 mM Magnesium chloride hexahydrate, 0.6 mM Calcium chloride, 2 mM Potassium phosphate dibasic, 60 uM Iron (II) chloride tetrahydrate, 15 uM Manganese (II) chloride tetrahydrate, 7.8 uM Cobalt chloride hexahydrate, 9 uM Zinc chloride, 1.26 uM Sodium molybdate, 1.92 uM Boric Acid, 2.28 uM Nickel (II) sulfate hexahydrate, 0.06 uM copper (II) chloride dihydrate, 0.21 uM Sodium selenite pentahydrate, 0.144 uM Sodium tungstate dihydrate, Thauer's vitamin mix (0.01 mg/L Pyridoxine HCl, 0.005 mg/L 4-Aminobenzoic acid, 0.005 mg/L Lipoic acid, 0.005 mg/L Nicotinic Acid, 0.005 mg/L Riboflavin, 0.005 mg/L Thiamine HCl, 0.005 mg/L calcium pantothenate, 0.002 mg/L biotin, 0.002 mg/L Folic Acid, 0.0001 mg/L Cyanocobalamin, 0.2 mg/L Choline chloride)

Specific Phenotypes

For 5 genes in this experiment

For stress Chloramphenicol in Desulfovibrio vulgaris Miyazaki F

For stress Chloramphenicol across organisms

SEED Subsystems

Subsystem #Specific
Folate Biosynthesis 1
Glycolysis and Gluconeogenesis 1
Glycolysis and Gluconeogenesis, including Archaeal enzymes 1
Pyruvate metabolism I: anaplerotic reactions, PEP 1
Type IV pilus 1
Zinc resistance 1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway #Steps #Present #Specific
glycolysis V (Pyrococcus) 10 7 1
glycolysis II (from fructose 6-phosphate) 11 10 1
glycolysis I (from glucose 6-phosphate) 13 11 1
gluconeogenesis I 13 10 1
superpathway of glycolysis and the Entner-Doudoroff pathway 17 12 1
superpathway of hexitol degradation (bacteria) 18 11 1
gluconeogenesis II (Methanobacterium thermoautotrophicum) 18 9 1
superpathway of anaerobic sucrose degradation 19 15 1
hexitol fermentation to lactate, formate, ethanol and acetate 19 15 1
superpathway of N-acetylneuraminate degradation 22 14 1
superpathway of glycolysis, pyruvate dehydrogenase, TCA, and glyoxylate bypass 26 18 1
Methanobacterium thermoautotrophicum biosynthetic metabolism 56 21 1