Experiment set1H30 for Marinobacter adhaerens HP15

Defined media with casamino acids as nitrogen source

Group: nitrogen source
Media: DinoMM_alphaKetoGlutAcid_NoNitrogen + casamino acids (0.1 mg/ml)
Culturing: Marino_ML2, 48 well microplate; Tecan Infinite F200, Aerobic, at 30 (C), shaken=orbital
By: Jordan on 9/5/2013
Media components: 20 g/L Sea salts, 0.1 g/L Potassium phosphate monobasic, 10 mM a-Ketoglutaric acid disodium salt hydrate, Wolfe's mineral mix (0.003 g/L Magnesium Sulfate Heptahydrate, 0.0015 g/L Nitrilotriacetic acid, 0.001 g/L Sodium Chloride, 0.0005 g/L Manganese (II) sulfate monohydrate, 0.0001 g/L Cobalt chloride hexahydrate, 0.0001 g/L Zinc sulfate heptahydrate, 0.0001 g/L Calcium chloride dihydrate, 0.0001 g/L Iron (II) sulfate heptahydrate, 2.5e-05 g/L Nickel (II) chloride hexahydrate, 2e-05 g/L Aluminum potassium sulfate dodecahydrate, 1e-05 g/L Copper (II) sulfate pentahydrate, 1e-05 g/L Boric Acid, 1e-05 g/L Sodium Molybdate Dihydrate, 0.0003 mg/L Sodium selenite pentahydrate), Wolfe's vitamin mix (0.05 mg/L Pyridoxine HCl, 0.025 mg/L 4-Aminobenzoic acid, 0.025 mg/L Lipoic acid, 0.025 mg/L Nicotinic Acid, 0.025 mg/L Riboflavin, 0.025 mg/L Thiamine HCl, 0.025 mg/L calcium pantothenate, 0.01 mg/L biotin, 0.01 mg/L Folic Acid, 0.0005 mg/L Cyanocobalamin)
Growth plate: 676 D5,D6

Specific Phenotypes

For 2 genes in this experiment

For nitrogen source casamino acids in Marinobacter adhaerens HP15

For nitrogen source casamino acids across organisms

SEED Subsystems

Subsystem	#Specific
Copper homeostasis	1

Metabolic Maps

Color code by fitness: see overview map or list of maps.