LB with Nickel (II) chloride hexahydrate 0.4 mM
Group:
stress
Media:
LB +
Nickel (II) chloride hexahydrate (0.4 mM)
Culturing: MR1_ML3, 48 well microplate; Tecan Infinite F200, Aerobic, at 30 (C), shaken=orbital
By: Adam on
8/18/2013
Media components: 10 g/L
Tryptone, 5 g/L
Yeast Extract, 5 g/L
Sodium Chloride
Growth plate: 647 A3,A4
Specific Phenotypes
For 25 genes in this experiment
For stress Nickel (II) chloride hexahydrate in Shewanella oneidensis MR-1
For stress Nickel (II) chloride hexahydrate across organisms
SEED Subsystems
Metabolic Maps
Color code by fitness: see overview map or list of maps.
Maps containing gene(s) with specific phenotypes:
MetaCyc Pathways
Pathways that contain genes with specific phenotypes:
| Pathway | #Steps | #Present | #Specific |
|---|
| long-chain fatty acid activation | 1 | 1 | 1 |
| superpathway of heme b biosynthesis from uroporphyrinogen-III | 6 | 6 | 3 |
| heme b biosynthesis V (aerobic) | 4 | 4 | 2 |
| heme b biosynthesis II (oxygen-independent) | 4 | 4 | 2 |
| heme b biosynthesis I (aerobic) | 4 | 4 | 2 |
| NADH to cytochrome bd oxidase electron transfer II | 2 | 2 | 1 |
| NADH to cytochrome bd oxidase electron transfer I | 2 | 2 | 1 |
| γ-linolenate biosynthesis II (animals) | 2 | 1 | 1 |
| NADH to cytochrome bo oxidase electron transfer II | 2 | 1 | 1 |
| NADH to nitrate electron transfer | 2 | 1 | 1 |
| NADH to cytochrome aa3 oxidase electron transfer | 2 | 1 | 1 |
| NADH to cytochrome bo oxidase electron transfer I | 2 | 1 | 1 |
| linoleate biosynthesis II (animals) | 2 | 1 | 1 |
| nitrate reduction VIIIb (dissimilatory) | 2 | 1 | 1 |
| aerobic respiration III (alternative oxidase pathway) | 3 | 2 | 1 |
| D-galactose detoxification | 3 | 2 | 1 |
| 3-methyl-branched fatty acid α-oxidation | 6 | 3 | 2 |
| alkane biosynthesis II | 3 | 1 | 1 |
| oleate biosynthesis I (plants) | 3 | 1 | 1 |
| β-1,4-D-mannosyl-N-acetyl-D-glucosamine degradation | 3 | 1 | 1 |
| superpathway of heme b biosynthesis from glycine | 8 | 7 | 2 |
| aerobic respiration I (cytochrome c) | 4 | 3 | 1 |
| aerobic respiration II (cytochrome c) (yeast) | 4 | 3 | 1 |
| phytol degradation | 4 | 3 | 1 |
| GDP-mannose biosynthesis | 4 | 2 | 1 |
| phosphatidylcholine acyl editing | 4 | 2 | 1 |
| wax esters biosynthesis II | 4 | 1 | 1 |
| long chain fatty acid ester synthesis (engineered) | 4 | 1 | 1 |
| sporopollenin precursors biosynthesis | 18 | 5 | 4 |
| superpathway of heme b biosynthesis from glutamate | 10 | 10 | 2 |
| D-galactose degradation I (Leloir pathway) | 5 | 4 | 1 |
| mitochondrial NADPH production (yeast) | 5 | 3 | 1 |
| sphingosine and sphingosine-1-phosphate metabolism | 10 | 4 | 2 |
| octane oxidation | 5 | 2 | 1 |
| fatty acid salvage | 6 | 6 | 1 |
| stearate biosynthesis II (bacteria and plants) | 6 | 5 | 1 |
| stearate biosynthesis IV | 6 | 4 | 1 |
| Fe(II) oxidation | 6 | 3 | 1 |
| NAD(P)/NADPH interconversion | 6 | 3 | 1 |
| 6-gingerol analog biosynthesis (engineered) | 6 | 2 | 1 |
| stearate biosynthesis I (animals) | 6 | 1 | 1 |
| stachyose degradation | 7 | 3 | 1 |
| ceramide degradation by α-oxidation | 7 | 2 | 1 |
| β-(1,4)-mannan degradation | 7 | 1 | 1 |
| arachidonate biosynthesis III (6-desaturase, mammals) | 7 | 1 | 1 |
| icosapentaenoate biosynthesis II (6-desaturase, mammals) | 7 | 1 | 1 |
| icosapentaenoate biosynthesis III (8-desaturase, mammals) | 7 | 1 | 1 |
| capsaicin biosynthesis | 7 | 1 | 1 |
| superpathway of NAD/NADP - NADH/NADPH interconversion (yeast) | 8 | 5 | 1 |
| 2-deoxy-D-ribose degradation II | 8 | 4 | 1 |
| ceramide and sphingolipid recycling and degradation (yeast) | 16 | 4 | 2 |
| 3,8-divinyl-chlorophyllide a biosynthesis I (aerobic, light-dependent) | 9 | 3 | 1 |
| 3,8-divinyl-chlorophyllide a biosynthesis II (anaerobic) | 9 | 3 | 1 |
| 3,8-divinyl-chlorophyllide a biosynthesis III (aerobic, light independent) | 9 | 3 | 1 |
| suberin monomers biosynthesis | 20 | 3 | 2 |
| superpathway of fatty acid biosynthesis II (plant) | 43 | 37 | 4 |
| colanic acid building blocks biosynthesis | 11 | 6 | 1 |
| superpathway of GDP-mannose-derived O-antigen building blocks biosynthesis | 14 | 4 | 1 |
| palmitate biosynthesis II (type II fatty acid synthase) | 31 | 29 | 2 |
| cutin biosynthesis | 16 | 2 | 1 |
| superpathway of bacteriochlorophyll a biosynthesis | 26 | 6 | 1 |
| superpathway of fatty acids biosynthesis (E. coli) | 53 | 48 | 2 |
| palmitate biosynthesis III | 29 | 21 | 1 |
| oleate β-oxidation | 35 | 32 | 1 |