Experiment set1H25 for Dinoroseobacter shibae DFL-12

Compare to:

marine broth with Benzalkonium Chloride 0.0001 mg/ml

Group: stress
Media: marine_broth_2216 + Benzalkonium Chloride (1e-04 mg/ml)
Culturing: Dino_ML3, 48 well microplate; Tecan Infinite F200, Aerobic, at 30 (C), shaken=orbital
By: Jordan on 8/16/2013
Media components: 5 g/L Bacto Peptone, 1 g/L Yeast Extract, 0.1 g/L Ferric citrate, 19.45 g/L Sodium Chloride, 5.9 g/L Magnesium chloride hexahydrate, 3.24 g/L Magnesium sulfate, 1.8 g/L Calcium chloride, 0.55 g/L Potassium Chloride, 0.16 g/L Sodium bicarbonate, 0.08 g/L Potassium bromide, 34 mg/L Strontium chloride, 22 mg/L Boric Acid, 4 mg/L Sodium metasilicate, 2.4 mg/L sodium fluoride, 8 mg/L Disodium phosphate
Growth plate: 643 A7,A8

Specific Phenotypes

For 13 genes in this experiment

For stress Benzalkonium Chloride in Dinoroseobacter shibae DFL-12

For stress Benzalkonium Chloride across organisms

SEED Subsystems

Subsystem #Specific
Biogenesis of cbb3-type cytochrome c oxidases 1
Capsular heptose biosynthesis 1
Colanic acid biosynthesis 1
Glycerol and Glycerol-3-phosphate Uptake and Utilization 1
Glycerolipid and Glycerophospholipid Metabolism in Bacteria 1
MLST 1
NAD and NADP cofactor biosynthesis global 1
Ton and Tol transport systems 1
Transcription initiation, bacterial sigma factors 1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway #Steps #Present #Specific
GDP-L-fucose biosynthesis I (from GDP-D-mannose) 3 3 2
glycerol degradation I 3 2 1
L-tryptophan degradation I (via anthranilate) 3 1 1
glycerol and glycerophosphodiester degradation 4 3 1
L-tryptophan degradation to 2-amino-3-carboxymuconate semialdehyde 5 1 1
3-hydroxy-4-methyl-anthranilate biosynthesis II 5 1 1
colanic acid building blocks biosynthesis 11 11 2
superpathway of GDP-mannose-derived O-antigen building blocks biosynthesis 14 9 2
NAD de novo biosynthesis II (from tryptophan) 9 4 1
L-tryptophan degradation XI (mammalian, via kynurenine) 23 5 2
L-tryptophan degradation XII (Geobacillus) 12 2 1
L-tryptophan degradation IX 12 2 1
superpathway of NAD biosynthesis in eukaryotes 14 6 1
L-tryptophan degradation III (eukaryotic) 15 4 1
superpathway of aromatic compound degradation via 3-oxoadipate 35 13 1
superpathway of aromatic compound degradation via 2-hydroxypentadienoate 42 10 1