Experiment set1H21 for Dinoroseobacter shibae DFL-12

Compare to:

marine broth with Sodium perchlorate monohydrate 50 mM

Group: stress
Media: marine_broth_2216 + Sodium perchlorate monohydrate (50 mM)
Culturing: Dino_ML3, 48 well microplate; Tecan Infinite F200, Aerobic, at 26 (C), shaken=orbital
By: Jordan on 8/16/2013
Media components: 5 g/L Bacto Peptone, 1 g/L Yeast Extract, 0.1 g/L Ferric citrate, 19.45 g/L Sodium Chloride, 5.9 g/L Magnesium chloride hexahydrate, 3.24 g/L Magnesium sulfate, 1.8 g/L Calcium chloride, 0.55 g/L Potassium Chloride, 0.16 g/L Sodium bicarbonate, 0.08 g/L Potassium bromide, 34 mg/L Strontium chloride, 22 mg/L Boric Acid, 4 mg/L Sodium metasilicate, 2.4 mg/L sodium fluoride, 8 mg/L Disodium phosphate
Growth plate: 642 D7,D8

Specific Phenotypes

For 8 genes in this experiment

For stress Sodium perchlorate monohydrate in Dinoroseobacter shibae DFL-12

For stress Sodium perchlorate monohydrate across organisms

SEED Subsystems

Subsystem #Specific
ABC transporter branched-chain amino acid (TC 3.A.1.4.1) 1
Glycine and Serine Utilization 1
Methionine Biosynthesis 1
Pyridoxin (Vitamin B6) Biosynthesis 1
Serine Biosynthesis 1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway #Steps #Present #Specific
taurine degradation I 1 1 1
L-homocysteine biosynthesis 2 2 1
L-serine biosynthesis I 3 3 1
hypotaurine degradation 3 3 1
superpathway of L-cysteine biosynthesis (fungi) 6 4 2
L-cysteine biosynthesis IX (Trichomonas vaginalis) 3 2 1
superpathway of L-serine and glycine biosynthesis I 4 4 1
L-methionine biosynthesis III 4 4 1
homocysteine and cysteine interconversion 4 2 1
superpathway of sulfur amino acid biosynthesis (Saccharomyces cerevisiae) 10 8 2
L-methionine biosynthesis I 5 4 1
S-methyl-5-thio-α-D-ribose 1-phosphate degradation III 5 2 1
S-methyl-5-thio-α-D-ribose 1-phosphate degradation II 5 2 1
L-methionine biosynthesis II 6 5 1
superpathway of taurine degradation 6 3 1
superpathway of L-homoserine and L-methionine biosynthesis 8 7 1
superpathway of sulfate assimilation and cysteine biosynthesis 9 9 1
superpathway of S-adenosyl-L-methionine biosynthesis 9 8 1
superpathway of L-methionine biosynthesis (transsulfuration) 9 8 1
superpathway of L-methionine biosynthesis (by sulfhydrylation) 12 12 1
superpathway of L-lysine, L-threonine and L-methionine biosynthesis II 15 13 1
superpathway of L-lysine, L-threonine and L-methionine biosynthesis I 18 17 1
aspartate superpathway 25 21 1