Experiment set1H19 for Dinoroseobacter shibae DFL-12

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marine broth with Sodium Chlorate 40 mM

Group: stress
Media: marine_broth_2216 + Sodium Chlorate (40 mM)
Culturing: Dino_ML3, 48 well microplate; Tecan Infinite F200, Aerobic, at 26 (C), shaken=orbital
By: Jordan on 8/16/2013
Media components: 5 g/L Bacto Peptone, 1 g/L Yeast Extract, 0.1 g/L Ferric citrate, 19.45 g/L Sodium Chloride, 5.9 g/L Magnesium chloride hexahydrate, 3.24 g/L Magnesium sulfate, 1.8 g/L Calcium chloride, 0.55 g/L Potassium Chloride, 0.16 g/L Sodium bicarbonate, 0.08 g/L Potassium bromide, 34 mg/L Strontium chloride, 22 mg/L Boric Acid, 4 mg/L Sodium metasilicate, 2.4 mg/L sodium fluoride, 8 mg/L Disodium phosphate
Growth plate: 642 C7,C8

Specific Phenotypes

For 16 genes in this experiment

For stress Sodium Chlorate in Dinoroseobacter shibae DFL-12

For stress Sodium Chlorate across organisms

SEED Subsystems

Subsystem #Specific
Acetoin, butanediol metabolism 1
Branched-Chain Amino Acid Biosynthesis 1
Flagellum 1
Leucine Biosynthesis 1
Nitrosative stress 1
Proteasome bacterial 1
Proteolysis in bacteria, ATP-dependent 1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway #Steps #Present #Specific
sulfide oxidation I (to sulfur globules) 1 1 1
L-leucine biosynthesis 6 6 2
pyruvate decarboxylation to acetyl CoA I 3 3 1
aliphatic glucosinolate biosynthesis, side chain elongation cycle 30 10 10
3-methylbutanol biosynthesis (engineered) 7 6 2
sulfide oxidation III (to sulfite) 4 3 1
superpathway of sulfide oxidation (Starkeya novella) 5 4 1
Salmonella enterica serotype O:3,10 O antigen biosynthesis 5 1 1
Porphyromonas gingivalis O-LPS antigen biosynthesis 6 1 1
Salmonella enterica serotype O:2 O antigen biosynthesis 6 1 1
Salmonella enterica serotype O:4 O antigen biosynthesis (group B1) 6 1 1
Salmonella enterica serotype O:9,46 O antigen biosynthesis 6 1 1
Salmonella enterica serotype O:9 O antigen biosynthesis 6 1 1
butanol and isobutanol biosynthesis (engineered) 8 3 1
Salmonella enterica serotype O:8 O antigen biosynthesis 8 1 1
superpathway of branched chain amino acid biosynthesis 17 17 2
Salmonella enterica serotype O:9,46,27 O antigen biosynthesis 9 1 1
superpathway of sulfide oxidation (Acidithiobacillus ferrooxidans) 10 7 1
superpathway of sulfide oxidation (phototrophic sulfur bacteria) 12 5 1
succinoglycan biosynthesis 14 1 1
superpathway of cytosolic glycolysis (plants), pyruvate dehydrogenase and TCA cycle 22 19 1