Culturing: MR1_ML3, 48 well microplate; Tecan Infinite F200, Aerobic, at 30 (C), shaken=orbital
| Pathway | #Steps | #Present | #Specific |
|---|
| arginine dependent acid resistance | 1 | 1 | 1 |
| siroheme biosynthesis | 4 | 4 | 3 |
| phosphatidylserine and phosphatidylethanolamine biosynthesis I | 2 | 2 | 1 |
| NADH to cytochrome bd oxidase electron transfer II | 2 | 2 | 1 |
| NADH to cytochrome bd oxidase electron transfer I | 2 | 2 | 1 |
| superoxide radicals degradation | 2 | 2 | 1 |
| putrescine biosynthesis I | 2 | 1 | 1 |
| NADH to cytochrome bo oxidase electron transfer I | 2 | 1 | 1 |
| NADH to cytochrome aa3 oxidase electron transfer | 2 | 1 | 1 |
| NADH to cytochrome bo oxidase electron transfer II | 2 | 1 | 1 |
| acetoacetate degradation (to acetyl CoA) | 2 | 1 | 1 |
| methanol oxidation to formaldehyde IV | 2 | 1 | 1 |
| L-arginine degradation III (arginine decarboxylase/agmatinase pathway) | 2 | 1 | 1 |
| NADH to nitrate electron transfer | 2 | 1 | 1 |
| nitrate reduction VIIIb (dissimilatory) | 2 | 1 | 1 |
| factor 430 biosynthesis | 7 | 3 | 3 |
| 5,6-dehydrokavain biosynthesis (engineered) | 10 | 6 | 4 |
| ethanol degradation IV | 3 | 3 | 1 |
| benzoyl-CoA biosynthesis | 3 | 3 | 1 |
| L-arginine degradation IV (arginine decarboxylase/agmatine deiminase pathway) | 3 | 3 | 1 |
| putrescine biosynthesis II | 3 | 3 | 1 |
| L-isoleucine degradation I | 6 | 5 | 2 |
| valproate β-oxidation | 9 | 6 | 3 |
| propanoate fermentation to 2-methylbutanoate | 6 | 4 | 2 |
| ketolysis | 3 | 2 | 1 |
| polyhydroxybutanoate biosynthesis | 3 | 2 | 1 |
| aerobic respiration III (alternative oxidase pathway) | 3 | 2 | 1 |
| 2-methyl-branched fatty acid β-oxidation | 14 | 11 | 4 |
| reactive oxygen species degradation | 4 | 4 | 1 |
| aerobic respiration II (cytochrome c) (yeast) | 4 | 3 | 1 |
| aerobic respiration I (cytochrome c) | 4 | 3 | 1 |
| cardiolipin and phosphatidylethanolamine biosynthesis (Xanthomonas) | 4 | 2 | 1 |
| (2S)-ethylmalonyl-CoA biosynthesis | 4 | 2 | 1 |
| spermidine biosynthesis III | 4 | 2 | 1 |
| superpathway of putrescine biosynthesis | 4 | 2 | 1 |
| oleate β-oxidation | 35 | 32 | 8 |
| (R)- and (S)-3-hydroxybutanoate biosynthesis (engineered) | 5 | 5 | 1 |
| fatty acid β-oxidation II (plant peroxisome) | 5 | 3 | 1 |
| glutaryl-CoA degradation | 5 | 3 | 1 |
| mitochondrial NADPH production (yeast) | 5 | 3 | 1 |
| 9-cis, 11-trans-octadecadienoyl-CoA degradation (isomerase-dependent, yeast) | 10 | 4 | 2 |
| ketogenesis | 5 | 2 | 1 |
| 4-hydroxybenzoate biosynthesis III (plants) | 5 | 2 | 1 |
| cob(II)yrinate a,c-diamide biosynthesis I (early cobalt insertion) | 15 | 3 | 3 |
| pyruvate fermentation to acetone | 5 | 1 | 1 |
| isopropanol biosynthesis (engineered) | 5 | 1 | 1 |
| fatty acid β-oxidation VII (yeast peroxisome) | 5 | 1 | 1 |
| ethylbenzene degradation (anaerobic) | 5 | 1 | 1 |
| pyruvate fermentation to hexanol (engineered) | 11 | 7 | 2 |
| (8E,10E)-dodeca-8,10-dienol biosynthesis | 11 | 6 | 2 |
| fatty acid salvage | 6 | 6 | 1 |
| pyruvate fermentation to butanol II (engineered) | 6 | 5 | 1 |
| NAD(P)/NADPH interconversion | 6 | 3 | 1 |
| Fe(II) oxidation | 6 | 3 | 1 |
| 10-trans-heptadecenoyl-CoA degradation (MFE-dependent, yeast) | 6 | 1 | 1 |
| 4-ethylphenol degradation (anaerobic) | 6 | 1 | 1 |
| jasmonic acid biosynthesis | 19 | 4 | 3 |
| cob(II)yrinate a,c-diamide biosynthesis II (late cobalt incorporation) | 13 | 2 | 2 |
| fatty acid β-oxidation I (generic) | 7 | 6 | 1 |
| acetyl-CoA fermentation to butanoate | 7 | 4 | 1 |
| fatty acid β-oxidation VI (mammalian peroxisome) | 7 | 3 | 1 |
| pyruvate fermentation to butanoate | 7 | 3 | 1 |
| mevalonate pathway I (eukaryotes and bacteria) | 7 | 1 | 1 |
| mevalonate pathway II (haloarchaea) | 7 | 1 | 1 |
| L-valine degradation I | 8 | 7 | 1 |
| superpathway of polyamine biosynthesis II | 8 | 6 | 1 |
| superpathway of NAD/NADP - NADH/NADPH interconversion (yeast) | 8 | 5 | 1 |
| pyruvate fermentation to butanol I | 8 | 4 | 1 |
| superpathway of polyamine biosynthesis I | 8 | 4 | 1 |
| 2-deoxy-D-ribose degradation II | 8 | 4 | 1 |
| 2-methylpropene degradation | 8 | 2 | 1 |
| mevalonate pathway IV (archaea) | 8 | 1 | 1 |
| isoprene biosynthesis II (engineered) | 8 | 1 | 1 |
| mevalonate pathway III (Thermoplasma) | 8 | 1 | 1 |
| androstenedione degradation I (aerobic) | 25 | 6 | 3 |
| superpathway of Clostridium acetobutylicum acidogenic fermentation | 9 | 5 | 1 |
| benzoate biosynthesis I (CoA-dependent, β-oxidative) | 9 | 3 | 1 |
| 4-oxopentanoate degradation | 9 | 2 | 1 |
| superpathway of testosterone and androsterone degradation | 28 | 6 | 3 |
| L-glutamate degradation V (via hydroxyglutarate) | 10 | 5 | 1 |
| superpathway of geranylgeranyldiphosphate biosynthesis I (via mevalonate) | 10 | 4 | 1 |
| 3-phenylpropanoate degradation | 10 | 4 | 1 |
| L-lysine fermentation to acetate and butanoate | 10 | 3 | 1 |
| methyl tert-butyl ether degradation | 10 | 2 | 1 |
| superpathway of cholesterol degradation I (cholesterol oxidase) | 42 | 8 | 4 |
| superpathway of L-arginine, putrescine, and 4-aminobutanoate degradation | 11 | 6 | 1 |
| ethylmalonyl-CoA pathway | 11 | 2 | 1 |
| superpathway of cholesterol degradation II (cholesterol dehydrogenase) | 47 | 8 | 4 |
| superpathway of phospholipid biosynthesis III (E. coli) | 12 | 10 | 1 |
| adenosylcobalamin biosynthesis I (anaerobic) | 36 | 18 | 3 |
| L-glutamate degradation VII (to butanoate) | 12 | 4 | 1 |
| 10-trans-heptadecenoyl-CoA degradation (reductase-dependent, yeast) | 12 | 2 | 1 |
| 10-cis-heptadecenoyl-CoA degradation (yeast) | 12 | 2 | 1 |
| superpathway of L-arginine and L-ornithine degradation | 13 | 8 | 1 |
| superpathway of cardiolipin biosynthesis (bacteria) | 13 | 8 | 1 |
| superpathway of Clostridium acetobutylicum solventogenic fermentation | 13 | 6 | 1 |
| (4Z,7Z,10Z,13Z,16Z)-docosapentaenoate biosynthesis (6-desaturase) | 13 | 2 | 1 |
| androstenedione degradation II (anaerobic) | 27 | 4 | 2 |
| superpathway of glyoxylate cycle and fatty acid degradation | 14 | 11 | 1 |
| docosahexaenoate biosynthesis III (6-desaturase, mammals) | 14 | 2 | 1 |
| L-tryptophan degradation III (eukaryotic) | 15 | 3 | 1 |
| glycerol degradation to butanol | 16 | 10 | 1 |
| crotonate fermentation (to acetate and cyclohexane carboxylate) | 16 | 3 | 1 |
| adenosylcobalamin biosynthesis II (aerobic) | 33 | 19 | 2 |
| superpathway of arginine and polyamine biosynthesis | 17 | 13 | 1 |
| superpathway of Clostridium acetobutylicum acidogenic and solventogenic fermentation | 17 | 8 | 1 |
| benzoate fermentation (to acetate and cyclohexane carboxylate) | 17 | 3 | 1 |
| cholesterol degradation to androstenedione I (cholesterol oxidase) | 17 | 2 | 1 |
| 3-hydroxypropanoate/4-hydroxybutanate cycle | 18 | 7 | 1 |
| toluene degradation VI (anaerobic) | 18 | 3 | 1 |
| sitosterol degradation to androstenedione | 18 | 1 | 1 |
| Spodoptera littoralis pheromone biosynthesis | 22 | 4 | 1 |
| cholesterol degradation to androstenedione II (cholesterol dehydrogenase) | 22 | 2 | 1 |
| superpathway of cholesterol degradation III (oxidase) | 49 | 4 | 2 |
| photosynthetic 3-hydroxybutanoate biosynthesis (engineered) | 26 | 21 | 1 |
| platensimycin biosynthesis | 26 | 6 | 1 |
| superpathway of ergosterol biosynthesis I | 26 | 3 | 1 |
| 1-butanol autotrophic biosynthesis (engineered) | 27 | 21 | 1 |
| superpathway of phospholipid biosynthesis II (plants) | 28 | 10 | 1 |
| superpathway of cholesterol biosynthesis | 38 | 3 | 1 |
| superpathway of L-lysine degradation | 43 | 8 | 1 |
| Methanobacterium thermoautotrophicum biosynthetic metabolism | 56 | 22 | 1 |