Experiment set19IT059 for Pseudomonas fluorescens SBW25-INTG

L-Glutamine (C)(N); with TAPS; with Sodium chloride

Group: stress
Media: MTM_noNitrogen_noCarbon + L-Glutamine (20 mM) + Sodium Chloride (400 mM), pH=8.5
Culturing: PseudoSBW25_INTG_ML3, 96 deep-well microplate; 1.2 mL volume, Aerobic, at 8 (C), shaken=1200 rpm
By: Joshua Elmore on September 1, 2021
Media components: 9.1 mM Potassium phosphate dibasic trihydrate, 20 mM TAPS sodium salt, 4.3 mM Sodium Chloride, 0.41 mM Magnesium Sulfate Heptahydrate, 0.07 mM Calcium chloride dihydrate, MME Trace Minerals (0.5 mg/L EDTA tetrasodium tetrahydrate salt, 2 mg/L Ferric chloride, 0.05 mg/L Boric Acid, 0.05 mg/L Zinc chloride, 0.03 mg/L copper (II) chloride dihydrate, 0.05 mg/L Manganese (II) chloride tetrahydrate, 0.05 mg/L Diammonium molybdate, 0.05 mg/L Cobalt chloride hexahydrate, 0.05 mg/L Nickel (II) chloride hexahydrate)

Specific Phenotypes

For 5 genes in this experiment

For stress L-Glutamine in Pseudomonas fluorescens SBW25-INTG

For stress L-Glutamine across organisms

SEED Subsystems

Subsystem	#Specific
DNA repair, bacterial	1

Metabolic Maps

Color code by fitness: see overview map or list of maps.