Experiment set19IT027 for Pseudomonas fluorescens SBW25-INTG
Glucose (C) and Glycine betaine (N); with TAPS
Group: no stress controlMedia: MTM_noNitrogen_noCarbon + D-Glucose (10 mM) + Betaine (10 mM), pH=8.5
Culturing: PseudoSBW25_INTG_ML3, 96 deep-well microplate; 1.2 mL volume, Aerobic, at 8 (C), shaken=1200 rpm
By: Joshua Elmore on September 1, 2021
Media components: 9.1 mM Potassium phosphate dibasic trihydrate, 20 mM TAPS sodium salt, 4.3 mM Sodium Chloride, 0.41 mM Magnesium Sulfate Heptahydrate, 0.07 mM Calcium chloride dihydrate, MME Trace Minerals (0.5 mg/L EDTA tetrasodium tetrahydrate salt, 2 mg/L Ferric chloride, 0.05 mg/L Boric Acid, 0.05 mg/L Zinc chloride, 0.03 mg/L copper (II) chloride dihydrate, 0.05 mg/L Manganese (II) chloride tetrahydrate, 0.05 mg/L Diammonium molybdate, 0.05 mg/L Cobalt chloride hexahydrate, 0.05 mg/L Nickel (II) chloride hexahydrate)
Specific Phenotypes
For 2 genes in this experiment
For no stress control D-Glucose in Pseudomonas fluorescens SBW25-INTG
For no stress control D-Glucose across organisms
SEED Subsystems
Metabolic Maps
Color code by fitness: see overview map or list of maps.
Maps containing gene(s) with specific phenotypes:
- Glycine, serine and threonine metabolism
- Cyanoamino acid metabolism
- One carbon pool by folate
- Methane metabolism
MetaCyc Pathways
Pathways that contain genes with specific phenotypes:
| Pathway | #Steps | #Present | #Specific |
|---|---|---|---|
| glycine biosynthesis I | 1 | 1 | 1 |
| dTMP de novo biosynthesis (mitochondrial) | 3 | 3 | 1 |
| glycine degradation | 3 | 3 | 1 |
| superpathway of L-serine and glycine biosynthesis I | 4 | 4 | 1 |
| glycine betaine degradation II (mammalian) | 4 | 1 | 1 |
| folate polyglutamylation | 5 | 4 | 1 |
| glycine betaine degradation III | 7 | 7 | 1 |
| glycine betaine degradation I | 8 | 6 | 1 |
| folate transformations III (E. coli) | 9 | 9 | 1 |
| photorespiration III | 9 | 5 | 1 |
| photorespiration I | 9 | 5 | 1 |
| photorespiration II | 10 | 6 | 1 |
| folate transformations II (plants) | 11 | 10 | 1 |
| folate transformations I | 13 | 9 | 1 |
| formaldehyde assimilation I (serine pathway) | 13 | 6 | 1 |
| purine nucleobases degradation II (anaerobic) | 24 | 16 | 1 |