Experiment set19IT014 for Burkholderia phytofirmans PsJN

Parafilmed volatile agar plate with Trichoderma atroviridae IMI

Group: agar plate interaction
Media: LB
Culturing: BFirm_ML3a, agar plate, Aerobic, at 30 (C), (Solid)
By: Cat_Manuel on 17-Aug-21
Media components: 10 g/L Tryptone, 5 g/L Yeast Extract, 5 g/L Sodium Chloride

Specific Phenotypes

For 8 genes in this experiment

SEED Subsystems

Subsystem	#Specific
Flagellum	2
Acetyl-CoA fermentation to Butyrate	1
Anaerobic respiratory reductases	1
Butanol Biosynthesis	1
Flagellar motility	1
Isobutyryl-CoA to Propionyl-CoA Module	1
Isoleucine degradation	1
Valine degradation	1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

• Fatty acid metabolism
• Valine, leucine and isoleucine degradation
• Butanoate metabolism

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway	#Steps	#Present	#Specific
Ac/N-end rule pathway	21	6	6
pyruvate fermentation to butanol II (engineered)	6	4	1
pyruvate fermentation to hexanol (engineered)	11	7	1
Arg/N-end rule pathway (eukaryotic)	14	8	1
1-butanol autotrophic biosynthesis (engineered)	27	19	1