Experiment set18S428 for Ralstonia solanacearum GMI1000

D-Glucose carbon source

Group: carbon source
Media: M63_quarter_strength_noCarbon + D-Glucose (10 mM), pH=7
Culturing: Ralstonia_GMI1000_ML2, tube, Aerobic, at 28 (C), shaken=250 rpm
By: Matthew Cope-Arguello on 11-Jul-24
Media components: 3.4 g/L Potassium phosphate monobasic, 0.5 g/L Ammonium Sulfate, 1.25e-05 g/L Iron (II) sulfate heptahydrate, 0.0517 mM Magnesium sulfate

Specific Phenotypes

For 6 genes in this experiment

For carbon source D-Glucose in Ralstonia solanacearum GMI1000

For carbon source D-Glucose across organisms

SEED Subsystems

Subsystem	#Specific
Xylose utilization	2
Entner-Doudoroff Pathway	1
Glycolysis and Gluconeogenesis	1
Lacto-N-Biose I and Galacto-N-Biose Metabolic Pathway	1
Lactose and Galactose Uptake and Utilization	1
N-linked Glycosylation in Bacteria	1
Rhamnose containing glycans	1
linker unit-arabinogalactan synthesis	1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

• Galactose metabolism
• Nucleotide sugars metabolism
• Glycolysis / Gluconeogenesis
• Starch and sucrose metabolism
• Streptomycin biosynthesis
• Biosynthesis of phenylpropanoids
• Biosynthesis of terpenoids and steroids
• Biosynthesis of alkaloids derived from shikimate pathway
• Biosynthesis of alkaloids derived from ornithine, lysine and nicotinic acid
• Biosynthesis of alkaloids derived from histidine and purine
• Biosynthesis of alkaloids derived from terpenoid and polyketide
• Biosynthesis of plant hormones

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway	#Steps	#Present	#Specific
UDP-N-acetyl-D-galactosamine biosynthesis I	1	1	1
UDP-α-D-galactose biosynthesis	1	1	1
trehalose degradation II (cytosolic)	2	2	1
UDP-α-D-galactofuranose biosynthesis	2	1	1
trehalose degradation I (low osmolarity)	2	1	1
trehalose degradation V	3	2	1
GDP-α-D-glucose biosynthesis	3	2	1
D-galactose detoxification	3	1	1
trehalose degradation IV	3	1	1
UDP-N-acetyl-D-galactosamine biosynthesis II	7	4	2
sucrose degradation III (sucrose invertase)	4	4	1
glucose and glucose-1-phosphate degradation	5	3	1
D-galactose degradation I (Leloir pathway)	5	2	1
superpathway of UDP-glucose-derived O-antigen building blocks biosynthesis	6	4	1
UDP-N-acetyl-D-glucosamine biosynthesis II	6	4	1
glycogen degradation II	6	3	1
stachyose degradation	7	2	1
sucrose biosynthesis II	8	5	1
glycogen degradation I	8	4	1
chitin biosynthesis	9	6	1
1,3-propanediol biosynthesis (engineered)	9	4	1
UDP-sugars interconversion	9	2	1
teichuronic acid biosynthesis (B. subtilis 168)	9	2	1
glycolysis III (from glucose)	11	10	1
colanic acid building blocks biosynthesis	11	7	1
homolactic fermentation	12	11	1
Bifidobacterium shunt	15	12	1
heterolactic fermentation	18	15	1
mycolyl-arabinogalactan-peptidoglycan complex biosynthesis	18	1	1
superpathway of mycolyl-arabinogalactan-peptidoglycan complex biosynthesis	33	12	1