Experiment set18S425 for Ralstonia solanacearum GMI1000
Sucrose carbon source
Group: carbon sourceMedia: M63_quarter_strength_noCarbon + Sucrose (10 mM), pH=7
Culturing: Ralstonia_GMI1000_ML2, tube, Aerobic, at 28 (C), shaken=250 rpm
By: Matthew Cope-Arguello on 11-Jul-24
Media components: 3.4 g/L Potassium phosphate monobasic, 0.5 g/L Ammonium Sulfate, 1.25e-05 g/L Iron (II) sulfate heptahydrate, 0.0517 mM Magnesium sulfate
Specific Phenotypes
For 4 genes in this experiment
For carbon source Sucrose in Ralstonia solanacearum GMI1000
For carbon source Sucrose across organisms
SEED Subsystems
| Subsystem | #Specific |
|---|---|
| Fructose utilization | 2 |
| Mannitol Utilization | 1 |
| Ribitol, Xylitol, Arabitol, Mannitol and Sorbitol utilization | 1 |
| Sucrose utilization | 1 |
| Sucrose utilization Shewanella | 1 |
Metabolic Maps
Color code by fitness: see overview map or list of maps.
Maps containing gene(s) with specific phenotypes:
- Fructose and mannose metabolism
- Pentose phosphate pathway
- Starch and sucrose metabolism
- Nucleotide sugars metabolism
- Lipopolysaccharide biosynthesis
- Nicotinate and nicotinamide metabolism
MetaCyc Pathways
Pathways that contain genes with specific phenotypes:
| Pathway | #Steps | #Present | #Specific |
|---|---|---|---|
| fructose degradation | 1 | 1 | 1 |
| D-sorbitol degradation I | 3 | 3 | 1 |
| sucrose degradation I (sucrose phosphotransferase) | 3 | 2 | 1 |
| sucrose degradation III (sucrose invertase) | 4 | 4 | 1 |
| sucrose degradation IV (sucrose phosphorylase) | 4 | 3 | 1 |
| D-galactosamine and N-acetyl-D-galactosamine degradation | 4 | 2 | 1 |
| sucrose degradation VII (sucrose 3-dehydrogenase) | 4 | 1 | 1 |
| sucrose degradation II (sucrose synthase) | 5 | 4 | 1 |
| galactitol degradation | 5 | 3 | 1 |
| N-acetyl-D-galactosamine degradation | 5 | 2 | 1 |
| lactose degradation I | 5 | 2 | 1 |
| mannitol cycle | 5 | 2 | 1 |
| heterolactic fermentation | 18 | 15 | 1 |
| superpathway of hexitol degradation (bacteria) | 18 | 14 | 1 |
| superpathway of anaerobic sucrose degradation | 19 | 16 | 1 |