Experiment set18S423 for Ralstonia solanacearum GMI1000

D-Galactose carbon source

Group: carbon source
Media: M63_quarter_strength_noCarbon + D-Galactose (10 mM), pH=7
Culturing: Ralstonia_GMI1000_ML2, tube, Aerobic, at 28 (C), shaken=250 rpm
By: Matthew Cope-Arguello on 11-Jul-24
Media components: 3.4 g/L Potassium phosphate monobasic, 0.5 g/L Ammonium Sulfate, 1.25e-05 g/L Iron (II) sulfate heptahydrate, 0.0517 mM Magnesium sulfate

Specific Phenotypes

For 8 genes in this experiment

For carbon source D-Galactose in Ralstonia solanacearum GMI1000

For carbon source D-Galactose across organisms

SEED Subsystems

Subsystem	#Specific
L-Arabinose utilization	3
D-galactonate catabolism	1
Fatty Acid Biosynthesis FASII	1
Lacto-N-Biose I and Galacto-N-Biose Metabolic Pathway	1
Lactose and Galactose Uptake and Utilization	1
N-linked Glycosylation in Bacteria	1
Rhamnose containing glycans	1
linker unit-arabinogalactan synthesis	1
mycolic acid synthesis	1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

• Galactose metabolism
• Nucleotide sugars metabolism
• Ascorbate and aldarate metabolism
• Fatty acid biosynthesis
• Puromycin biosynthesis
• alpha-Linolenic acid metabolism
• Trinitrotoluene degradation
• Porphyrin and chlorophyll metabolism
• Carotenoid biosynthesis - General
• Alkaloid biosynthesis I
• Insect hormone biosynthesis
• Biosynthesis of unsaturated fatty acids

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway	#Steps	#Present	#Specific
UDP-α-D-galactose biosynthesis	1	1	1
UDP-N-acetyl-D-galactosamine biosynthesis I	1	1	1
iso-bile acids biosynthesis I	4	2	2
UDP-α-D-galactofuranose biosynthesis	2	1	1
D-galactonate degradation	3	3	1
D-galactose detoxification	3	1	1
octanoyl-[acyl-carrier protein] biosynthesis (mitochondria, yeast)	12	12	3
gondoate biosynthesis (anaerobic)	4	4	1
bile acids 3-O-epimerization	4	1	1
palmitate biosynthesis III	29	28	7
tetradecanoate biosynthesis (mitochondria)	25	23	6
palmitate biosynthesis II (type II fatty acid synthase)	31	29	7
palmitoleate biosynthesis I (from (5Z)-dodec-5-enoate)	9	9	2
oleate biosynthesis IV (anaerobic)	14	13	3
superpathway of fatty acids biosynthesis (E. coli)	53	50	11
fatty acid elongation -- saturated	5	5	1
cis-vaccenate biosynthesis	5	5	1
superpathway of unsaturated fatty acids biosynthesis (E. coli)	20	19	4
8-amino-7-oxononanoate biosynthesis IV	5	4	1
D-galactose degradation I (Leloir pathway)	5	2	1
superpathway of fatty acid biosynthesis II (plant)	43	38	8
8-amino-7-oxononanoate biosynthesis I	11	9	2
odd iso-branched-chain fatty acid biosynthesis	34	30	6
even iso-branched-chain fatty acid biosynthesis	34	30	6
anteiso-branched-chain fatty acid biosynthesis	34	30	6
stearate biosynthesis II (bacteria and plants)	6	5	1
(5Z)-dodecenoate biosynthesis I	6	5	1
superpathway of UDP-glucose-derived O-antigen building blocks biosynthesis	6	4	1
stearate biosynthesis IV	6	4	1
(5Z)-dodecenoate biosynthesis II	6	4	1
L-arabinose degradation III	6	3	1
L-arabinose degradation V	6	2	1
petroselinate biosynthesis	6	2	1
streptorubin B biosynthesis	34	20	5
UDP-N-acetyl-D-galactosamine biosynthesis II	7	4	1
stachyose degradation	7	2	1
biotin biosynthesis I	15	13	2
superpathway of fatty acid biosynthesis I (E. coli)	16	14	2
L-arabinose degradation IV	8	5	1
2-allylmalonyl-CoA biosynthesis	8	2	1
UDP-sugars interconversion	9	2	1
teichuronic acid biosynthesis (B. subtilis 168)	9	2	1
colanic acid building blocks biosynthesis	11	7	1
bile acids epimerization	12	1	1
mycolyl-arabinogalactan-peptidoglycan complex biosynthesis	18	1	1
superpathway of mycolyl-arabinogalactan-peptidoglycan complex biosynthesis	33	12	1
mycolate biosynthesis	205	25	5
superpathway of pentose and pentitol degradation	42	12	1
superpathway of mycolate biosynthesis	239	26	5