Experiment set18S409 for Ralstonia solanacearum GMI1000

L-Glutamic acid monopotassium salt monohydrate carbon source

Group: carbon source
Media: M63_quarter_strength_noCarbon + L-Glutamic acid monopotassium salt monohydrate (10 mM), pH=7
Culturing: Ralstonia_GMI1000_ML2, tube, Aerobic, at 28 (C), shaken=250 rpm
By: Matthew Cope-Arguello on 10-Jul-24
Media components: 3.4 g/L Potassium phosphate monobasic, 0.5 g/L Ammonium Sulfate, 1.25e-05 g/L Iron (II) sulfate heptahydrate, 0.0517 mM Magnesium sulfate

Specific Phenotypes

For 1 genes in this experiment

For carbon source L-Glutamic acid monopotassium salt monohydrate in Ralstonia solanacearum GMI1000

For carbon source L-Glutamic acid monopotassium salt monohydrate across organisms

SEED Subsystems

Subsystem	#Specific
Branched-Chain Amino Acid Biosynthesis	1
Leucine Biosynthesis	1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

• Valine, leucine and isoleucine biosynthesis
• Pyruvate metabolism
• Biosynthesis of alkaloids derived from histidine and purine

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway	#Steps	#Present	#Specific
L-leucine biosynthesis	6	6	1
3-methylbutanol biosynthesis (engineered)	7	6	1
superpathway of branched chain amino acid biosynthesis	17	17	1