Experiment set18IT044 for Klebsiella michiganensis M5al

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D-Glucose carbon source with ammonium chloride; anaerobic; 8 days

Group: nitrogen fixation control
Media: RCH2_defined_noCarbon + D-Glucose (20 mM)
Culturing: Koxy_ML2, serum bottle, Anaerobic, at 30 (C), shaken=0 rpm
Growth: about 3.8 generations
By: Kelly Wetmore; Jordan Baker on 26-Mar-19
Media components: 0.25 g/L Ammonium chloride, 0.1 g/L Potassium Chloride, 0.6 g/L Sodium phosphate monobasic monohydrate, 30 mM PIPES sesquisodium salt, Wolfe's mineral mix (0.03 g/L Magnesium Sulfate Heptahydrate, 0.015 g/L Nitrilotriacetic acid, 0.01 g/L Sodium Chloride, 0.005 g/L Manganese (II) sulfate monohydrate, 0.001 g/L Cobalt chloride hexahydrate, 0.001 g/L Zinc sulfate heptahydrate, 0.001 g/L Calcium chloride dihydrate, 0.001 g/L Iron (II) sulfate heptahydrate, 0.00025 g/L Nickel (II) chloride hexahydrate, 0.0002 g/L Aluminum potassium sulfate dodecahydrate, 0.0001 g/L Copper (II) sulfate pentahydrate, 0.0001 g/L Boric Acid, 0.0001 g/L Sodium Molybdate Dihydrate, 0.003 mg/L Sodium selenite pentahydrate), Wolfe's vitamin mix (0.1 mg/L Pyridoxine HCl, 0.05 mg/L 4-Aminobenzoic acid, 0.05 mg/L Lipoic acid, 0.05 mg/L Nicotinic Acid, 0.05 mg/L Riboflavin, 0.05 mg/L Thiamine HCl, 0.05 mg/L calcium pantothenate, 0.02 mg/L biotin, 0.02 mg/L Folic Acid, 0.001 mg/L Cyanocobalamin)

Specific Phenotypes

For 14 genes in this experiment

For nitrogen fixation control D-Glucose in Klebsiella michiganensis M5al

For nitrogen fixation control D-Glucose across organisms

SEED Subsystems

Subsystem #Specific
Succinate dehydrogenase 4
Cysteine Biosynthesis 3
Ribonucleotide reduction 2
Serine-glyoxylate cycle 2
TCA Cycle 2
Carboxysome 1
Cyanate hydrolysis 1
Fermentations: Mixed acid 1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway #Steps #Present #Specific
ethanol degradation I 2 2 2
acetaldehyde biosynthesis I 1 1 1
demethylmenaquinol-9 biosynthesis 1 1 1
demethylmenaquinol-4 biosynthesis 1 1 1
demethylmenaquinol-6 biosynthesis I 1 1 1
demethylmenaquinol-8 biosynthesis I 1 1 1
pyruvate fermentation to ethanol I 3 3 2
pyruvate fermentation to ethanol III 3 3 2
CO2 fixation into oxaloacetate (anaplerotic) 2 2 1
L-threonine degradation IV 2 2 1
NADH to fumarate electron transfer 2 2 1
glycerol-3-phosphate to fumarate electron transfer 2 2 1
pyruvate fermentation to ethanol II 2 2 1
succinate to cytochrome bd oxidase electron transfer 2 2 1
succinate to cytochrome bo oxidase electron transfer 2 1 1
menaquinol-10 biosynthesis 2 1 1
menaquinol-13 biosynthesis 2 1 1
menaquinol-11 biosynthesis 2 1 1
succinate to cytochrome aa3 oxidase electron transfer 2 1 1
menaquinol-12 biosynthesis 2 1 1
hydrogen to fumarate electron transfer 2 1 1
menaquinol-7 biosynthesis 2 1 1
ethanolamine utilization 5 5 2
acetylene degradation (anaerobic) 5 4 2
2-hydroxypenta-2,4-dienoate degradation 3 3 1
aerobic respiration III (alternative oxidase pathway) 3 3 1
molybdenum cofactor biosynthesis 3 3 1
ethanol degradation II 3 3 1
2-deoxy-D-ribose degradation I 3 3 1
2-deoxy-α-D-ribose 1-phosphate degradation 3 3 1
L-isoleucine degradation II 3 2 1
L-methionine degradation III 3 2 1
cyanate degradation 3 2 1
L-valine degradation II 3 2 1
L-leucine degradation III 3 2 1
2-aminoethylphosphonate degradation I 3 1 1
sulfoacetaldehyde degradation IV 3 1 1
pyrimidine deoxyribonucleotides de novo biosynthesis II 7 7 2
adenosine deoxyribonucleotides de novo biosynthesis II 4 4 1
guanosine deoxyribonucleotides de novo biosynthesis II 4 4 1
phytol degradation 4 3 1
salidroside biosynthesis 4 2 1
L-phenylalanine degradation III 4 2 1
aerobic respiration I (cytochrome c) 4 2 1
L-tyrosine degradation III 4 2 1
aerobic respiration II (cytochrome c) (yeast) 4 2 1
superpathway of fermentation (Chlamydomonas reinhardtii) 9 9 2
(S)-propane-1,2-diol degradation 5 5 1
pyruvate fermentation to isobutanol (engineered) 5 4 1
catechol degradation I (meta-cleavage pathway) 5 4 1
phenylethanol biosynthesis 5 2 1
mixed acid fermentation 16 16 3
superpathway of pyrimidine deoxyribonucleosides degradation 6 6 1
triethylamine degradation 6 1 1
superpathway of Clostridium acetobutylicum solventogenic fermentation 13 7 2
noradrenaline and adrenaline degradation 13 4 2
superpathway of pyrimidine deoxyribonucleotides de novo biosynthesis (E. coli) 14 14 2
superpathway of adenosine nucleotides de novo biosynthesis II 7 7 1
superpathway of purine deoxyribonucleosides degradation 7 7 1
3-methylbutanol biosynthesis (engineered) 7 6 1
incomplete reductive TCA cycle 7 5 1
pyruvate fermentation to propanoate I 7 5 1
catechol degradation II (meta-cleavage pathway) 7 4 1
C4 photosynthetic carbon assimilation cycle, NADP-ME type 7 4 1
toluene degradation I (aerobic) (via o-cresol) 7 4 1
toluene degradation V (aerobic) (via toluene-cis-diol) 7 4 1
serotonin degradation 7 3 1
superpathway of guanosine nucleotides de novo biosynthesis II 8 8 1
3-phenylpropanoate and 3-(3-hydroxyphenyl)propanoate degradation 8 6 1
p-cumate degradation 8 3 1
butanol and isobutanol biosynthesis (engineered) 8 3 1
superpathway of Clostridium acetobutylicum acidogenic and solventogenic fermentation 17 9 2
superpathway of demethylmenaquinol-8 biosynthesis I 9 9 1
heterolactic fermentation 18 16 2
superpathway of demethylmenaquinol-9 biosynthesis 9 8 1
superpathway of demethylmenaquinol-6 biosynthesis I 9 8 1
TCA cycle VI (Helicobacter) 9 7 1
hexitol fermentation to lactate, formate, ethanol and acetate 19 19 2
superpathway of menaquinol-8 biosynthesis I 10 10 1
superpathway of menaquinol-12 biosynthesis 10 8 1
superpathway of menaquinol-10 biosynthesis 10 8 1
superpathway of menaquinol-11 biosynthesis 10 8 1
superpathway of menaquinol-13 biosynthesis 10 8 1
superpathway of menaquinol-7 biosynthesis 10 8 1
superpathway of menaquinol-9 biosynthesis 10 8 1
superpathway of menaquinol-6 biosynthesis 10 8 1
TCA cycle III (animals) 10 8 1
meta cleavage pathway of aromatic compounds 10 6 1
superpathway of N-acetylneuraminate degradation 22 22 2
reductive TCA cycle I 11 8 1
C4 photosynthetic carbon assimilation cycle, NAD-ME type 11 8 1
L-glutamate degradation VIII (to propanoate) 11 7 1
p-cymene degradation 11 3 1
reductive TCA cycle II 12 7 1
naphthalene degradation to acetyl-CoA 12 5 1
L-tryptophan degradation XII (Geobacillus) 12 4 1
L-tryptophan degradation IX 12 4 1
superpathway of purine nucleotides de novo biosynthesis II 26 26 2
(S)-lactate fermentation to propanoate, acetate and hydrogen 13 10 1
toluene degradation IV (aerobic) (via catechol) 13 7 1
3-hydroxypropanoate cycle 13 7 1
glyoxylate assimilation 13 5 1
L-tryptophan degradation V (side chain pathway) 13 1 1
C4 photosynthetic carbon assimilation cycle, PEPCK type 14 9 1
superpathway of L-threonine metabolism 18 18 1
mandelate degradation to acetyl-CoA 18 14 1
3-hydroxypropanoate/4-hydroxybutanate cycle 18 10 1
gluconeogenesis II (Methanobacterium thermoautotrophicum) 18 9 1
superpathway of the 3-hydroxypropanoate cycle 18 7 1
superpathway of anaerobic sucrose degradation 19 18 1
superpathway of histidine, purine, and pyrimidine biosynthesis 46 46 2
Methanobacterium thermoautotrophicum biosynthetic metabolism 56 23 2
superpathway of aerobic toluene degradation 30 15 1
superpathway of aromatic compound degradation via 3-oxoadipate 35 23 1
superpathway of aromatic compound degradation via 2-hydroxypentadienoate 42 17 1
superpathway of chorismate metabolism 59 54 1