Experiment set18IT034 for Burkholderia phytofirmans PsJN

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Plant=switchgrass; PreTreatment=None; PlantTreatment=low_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=21days

Group: in planta
Media: MS_Basal_Salts_Robin_LowPhosphate_0.5x + Plant=switchgrass; PreTreatment=None; PlantTreatment=low_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=21days
Culturing: BFirm_ML3a, pot
By: Robin on 7/2/21
Media components: 825 mg/L Ammonium Nitrate, 3.1 mg/L Boric Acid, 166.1 mg/L Calcium chloride, 0.0125 mg/L Cobalt chloride hexahydrate, 0.0125 mg/L Copper (II) sulfate pentahydrate, 18.63 mg/L EDTA (disodium salt), 13.9 mg/L Iron (II) sulfate heptahydrate, 90.35 mg/L Magnesium sulfate, 8.45 mg/L Manganese sulfate, 0.415 mg/L Potassium iodide, 950 mg/L Potassium nitrate, 30 uM Potassium phosphate monobasic, 4.3 mg/L Zinc sulfate heptahydrate

Specific Phenotypes

For 25 genes in this experiment

For in planta Plant=switchgrass; PreTreatment=None; PlantTreatment=low_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=21days in Burkholderia phytofirmans PsJN

For in planta Plant=switchgrass; PreTreatment=None; PlantTreatment=low_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=21days across organisms

SEED Subsystems

Subsystem #Specific
DNA repair, UvrABC system 2
Flagellar motility 2
Flagellum 2
High affinity phosphate transporter and control of PHO regulon 2
Phosphate metabolism 2
ABC transporter branched-chain amino acid (TC 3.A.1.4.1) 1
Acetyl-CoA fermentation to Butyrate 1
Bacterial Chemotaxis 1
Bacterial hemoglobins 1
D-ribose utilization 1
Maltose and Maltodextrin Utilization 1
Ribitol, Xylitol, Arabitol, Mannitol and Sorbitol utilization 1
Soluble cytochromes and functionally related electron carriers 1
Trehalose Biosynthesis 1
Two-component regulatory systems in Campylobacter 1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway #Steps #Present #Specific
D-arabinitol degradation I 2 2 1
sulfoacetaldehyde degradation I 2 2 1
sulfolactate degradation II 4 2 1
arsenic detoxification (bacteria) 4 2 1
arsenate detoxification I 6 4 1
superpathway of taurine degradation 6 3 1
superpathway of sulfolactate degradation 6 3 1
picolinate degradation 7 2 1
superpathway of pentose and pentitol degradation 42 22 1