Experiment set17S922 for Phocaeicola vulgatus CL09T03C04

Starch soluble 5 mg/ml carbon source mhCom BHIS_K3_noGlucose Passage2

Group: mixed community
Media: BHIS_K3_noGlucose + Starch soluble (5 mg/ml) + mhCom
Culturing: Bvulgatus_CL09T03C04_ML5, 96 deep-well microplate; 1.2 mL volume, Anaerobic, at 37 (C), shaken=0 rpm
By: Surya on 10/8/25
Media components: 7.7 g/L Calf brains, 9.8 g/L Beef heart, 10 g/L Proteose Peptone, 5 g/L Sodium Chloride, 2.5 g/L Disodium phosphate, 1 g/L L-Cysteine, 2 g/L Sodium bicarbonate, 0.005 g/L Hemin, 0.001 g/L Menadione

Specific Phenotypes

For 7 genes in this experiment

For mixed community Starch soluble in Phocaeicola vulgatus CL09T03C04

For mixed community Starch soluble across organisms

SEED Subsystems

Subsystem	#Specific
Fermentations: Mixed acid	2
Butanol Biosynthesis	1
Galactosylceramide and Sulfatide metabolism	1
Lactose and Galactose Uptake and Utilization	1
Lactose utilization	1
Threonine anaerobic catabolism gene cluster	1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

• Galactose metabolism
• 1,1,1-Trichloro-2,2-bis(4-chlorophenyl)ethane (DDT) degradation
• gamma-Hexachlorocyclohexane degradation
• Bisphenol A degradation
• Other glycan degradation
• Glycosaminoglycan degradation
• Sphingolipid metabolism
• Glycosphingolipid biosynthesis - ganglio series
• Pyruvate metabolism
• Tetrachloroethene degradation
• 1,4-Dichlorobenzene degradation
• Propanoate metabolism
• Butanoate metabolism

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway	#Steps	#Present	#Specific
lactose degradation III	1	1	1
reductive monocarboxylic acid cycle	2	2	1
xyloglucan degradation II (exoglucanase)	8	5	3
pyruvate fermentation to acetate IV	3	3	1
pyruvate fermentation to ethanol I	3	2	1
L-threonine degradation I	6	6	1
superpathway of fermentation (Chlamydomonas reinhardtii)	9	7	1
(S)-lactate fermentation to propanoate, acetate and hydrogen	13	10	1
mixed acid fermentation	16	12	1
superpathway of L-threonine metabolism	18	15	1
hexitol fermentation to lactate, formate, ethanol and acetate	19	14	1
superpathway of N-acetylneuraminate degradation	22	17	1