Experiment set17IT065 for Pseudomonas stutzeri RCH2
LB with L-Lysine 200 mM
Group: stressMedia: LB + L-Lysine (200 mM)
Culturing: psRCH2_ML7c, 48 well microplate; Tecan Infinite F200, Aerobic, at 28 (C), shaken=orbital
By: Kelly on 4/17/2014
Media components: 10 g/L Tryptone, 5 g/L Yeast Extract, 5 g/L Sodium Chloride
Growth plate: 885 F5,F6
Specific Phenotypes
For 16 genes in this experiment
For stress L-Lysine in Pseudomonas stutzeri RCH2
For stress L-Lysine across organisms
SEED Subsystems
| Subsystem | #Specific |
|---|---|
| Choline and Betaine Uptake and Betaine Biosynthesis | 1 |
| Ectoine biosynthesis and regulation | 1 |
| Iron acquisition in Vibrio | 1 |
| N-linked Glycosylation in Bacteria | 1 |
| Transport of Iron | 1 |
Metabolic Maps
Color code by fitness: see overview map or list of maps.
Maps containing gene(s) with specific phenotypes:
- Nucleotide sugars metabolism
- Ascorbate and aldarate metabolism
- Fatty acid biosynthesis
- Ubiquinone and menaquinone biosynthesis
- Glycine, serine and threonine metabolism
- Methionine metabolism
- Lysine biosynthesis
- Lysine degradation
- Arginine and proline metabolism
- Tyrosine metabolism
- Benzoate degradation via hydroxylation
- Phenylalanine, tyrosine and tryptophan biosynthesis
- Aminophosphonate metabolism
- Glycosaminoglycan degradation
- 1- and 2-Methylnaphthalene degradation
- Benzoate degradation via CoA ligation
- Butanoate metabolism
- Porphyrin and chlorophyll metabolism
- Limonene and pinene degradation
- Caprolactam degradation
- Biosynthesis of unsaturated fatty acids
- Biosynthesis of type II polyketide backbone
MetaCyc Pathways
Pathways that contain genes with specific phenotypes:
| Pathway | #Steps | #Present | #Specific |
|---|---|---|---|
| alcaligin biosynthesis | 5 | 3 | 2 |
| UDP-N-acetyl-β-L-quinovosamine biosynthesis | 4 | 1 | 1 |
| UDP-N-acetyl-β-L-fucosamine biosynthesis | 4 | 1 | 1 |
| ectoine degradation | 4 | 1 | 1 |
| ectoine biosynthesis | 5 | 5 | 1 |
| CMP-pseudaminate biosynthesis | 6 | 6 | 1 |
| norspermidine biosynthesis | 6 | 4 | 1 |
| rhizobactin 1021 biosynthesis | 7 | 1 | 1 |
| CMP-diacetamido-8-epilegionaminic acid biosynthesis | 7 | 1 | 1 |
| superpathway of polyamine biosynthesis III | 8 | 5 | 1 |
| peptidoglycan recycling II | 10 | 7 | 1 |
| pyoverdine I biosynthesis | 11 | 2 | 1 |
| baumannoferrin biosynthesis | 11 | 1 | 1 |
| peptidoglycan recycling I | 14 | 9 | 1 |
| superpathway of UDP-N-acetylglucosamine-derived O-antigen building blocks biosynthesis | 24 | 9 | 1 |