Experiment set17IT036 for Sinorhizobium meliloti 1021

methyl 3-keto-a-D-glucopyranoside carbon source

Group: carbon source
Media: RCH2_defined_noCarbon + methyl 3-keto-a-D-glucopyranoside (5 mM)
Culturing: Smeli_ML6a, 24-well transparent microplate; Multitron, Aerobic, at 30 (C), shaken=700 rpm
By: Valentine on 10/5/22
Media components: 0.25 g/L Ammonium chloride, 0.1 g/L Potassium Chloride, 0.6 g/L Sodium phosphate monobasic monohydrate, 30 mM PIPES sesquisodium salt, Wolfe's mineral mix (0.03 g/L Magnesium Sulfate Heptahydrate, 0.015 g/L Nitrilotriacetic acid, 0.01 g/L Sodium Chloride, 0.005 g/L Manganese (II) sulfate monohydrate, 0.001 g/L Cobalt chloride hexahydrate, 0.001 g/L Zinc sulfate heptahydrate, 0.001 g/L Calcium chloride dihydrate, 0.001 g/L Iron (II) sulfate heptahydrate, 0.00025 g/L Nickel (II) chloride hexahydrate, 0.0002 g/L Aluminum potassium sulfate dodecahydrate, 0.0001 g/L Copper (II) sulfate pentahydrate, 0.0001 g/L Boric Acid, 0.0001 g/L Sodium Molybdate Dihydrate, 0.003 mg/L Sodium selenite pentahydrate), Wolfe's vitamin mix (0.1 mg/L Pyridoxine HCl, 0.05 mg/L 4-Aminobenzoic acid, 0.05 mg/L Lipoic acid, 0.05 mg/L Nicotinic Acid, 0.05 mg/L Riboflavin, 0.05 mg/L Thiamine HCl, 0.05 mg/L calcium pantothenate, 0.02 mg/L biotin, 0.02 mg/L Folic Acid, 0.001 mg/L Cyanocobalamin)

Specific Phenotypes

For 4 genes in this experiment

For carbon source methyl 3-keto-a-D-glucopyranoside in Sinorhizobium meliloti 1021

For carbon source methyl 3-keto-a-D-glucopyranoside across organisms

SEED Subsystems

Subsystem	#Specific
Maltose and Maltodextrin Utilization	2
Bacterial Chemotaxis	1
Inositol catabolism	1
Pentose phosphate pathway	1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

• Pentose phosphate pathway
• Glutathione metabolism
• 1- and 2-Methylnaphthalene degradation
• Biosynthesis of alkaloids derived from histidine and purine
• Biosynthesis of plant hormones

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway	#Steps	#Present	#Specific
pentose phosphate pathway (oxidative branch) I	3	3	1
pentose phosphate pathway	8	8	1
superpathway of glucose and xylose degradation	17	15	1