Experiment set17IT030 for Pseudomonas stutzeri RCH2

LB with L-Lysine 200 mM

Group: stress
Media: LB + L-Lysine (200 mM)
Culturing: psRCH2_ML7c, 48 well microplate; Tecan Infinite F200, Aerobic, at 28 (C), shaken=orbital
By: Kelly on 4/17/2014
Media components: 10 g/L Tryptone, 5 g/L Yeast Extract, 5 g/L Sodium Chloride
Growth plate: 884 F5,F6

Specific Phenotypes

For 13 genes in this experiment

For stress L-Lysine in Pseudomonas stutzeri RCH2

For stress L-Lysine across organisms

SEED Subsystems

Subsystem	#Specific
Bacterial Chemotaxis	1
Choline and Betaine Uptake and Betaine Biosynthesis	1
Flagellar motility	1
Glycerolipid and Glycerophospholipid Metabolism in Bacteria	1
Iron acquisition in Vibrio	1
N-linked Glycosylation in Bacteria	1
Transport of Iron	1
Two-component regulatory systems in Campylobacter	1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

• Nucleotide sugars metabolism
• Fatty acid biosynthesis
• Tyrosine metabolism
• Benzoate degradation via hydroxylation
• Phenylalanine, tyrosine and tryptophan biosynthesis
• Aminophosphonate metabolism
• High-mannose type N-glycan biosynthesis
• Glycerophospholipid metabolism
• Sphingolipid metabolism
• 1- and 2-Methylnaphthalene degradation
• Benzoate degradation via CoA ligation
• Butanoate metabolism
• Porphyrin and chlorophyll metabolism
• Limonene and pinene degradation
• Biosynthesis of unsaturated fatty acids
• Biosynthesis of type II polyketide backbone

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway	#Steps	#Present	#Specific
alcaligin biosynthesis	5	3	2
cardiolipin biosynthesis II	3	3	1
cardiolipin and phosphatidylethanolamine biosynthesis (Xanthomonas)	4	3	1
UDP-N-acetyl-β-L-fucosamine biosynthesis	4	1	1
UDP-N-acetyl-β-L-quinovosamine biosynthesis	4	1	1
CMP-pseudaminate biosynthesis	6	6	1
CMP-diacetamido-8-epilegionaminic acid biosynthesis	7	1	1
superpathway of cardiolipin biosynthesis (bacteria)	13	9	1
superpathway of UDP-N-acetylglucosamine-derived O-antigen building blocks biosynthesis	24	9	1