Experiment set17IT018 for Klebsiella michiganensis M5al

Compare to:

RCH2_defined_1percent_glucose with Sodium Chloride 600 mM

Group: stress
Media: RCH2_defined_1percent_glucose + Sodium Chloride (600 mM)
Culturing: Koxy_ML2a, 24-well transparent microplate; Multitron, Aerobic, at 30 (C), shaken=700 rpm
By: Robin Herbert on 3/21/19
Media components: 0.25 g/L Ammonium chloride, 0.1 g/L Potassium Chloride, 0.6 g/L Sodium phosphate monobasic monohydrate, 55.5 mM D-Glucose, 30 mM PIPES sesquisodium salt, Wolfe's mineral mix (0.03 g/L Magnesium Sulfate Heptahydrate, 0.015 g/L Nitrilotriacetic acid, 0.01 g/L Sodium Chloride, 0.005 g/L Manganese (II) sulfate monohydrate, 0.001 g/L Cobalt chloride hexahydrate, 0.001 g/L Zinc sulfate heptahydrate, 0.001 g/L Calcium chloride dihydrate, 0.001 g/L Iron (II) sulfate heptahydrate, 0.00025 g/L Nickel (II) chloride hexahydrate, 0.0002 g/L Aluminum potassium sulfate dodecahydrate, 0.0001 g/L Copper (II) sulfate pentahydrate, 0.0001 g/L Boric Acid, 0.0001 g/L Sodium Molybdate Dihydrate, 0.003 mg/L Sodium selenite pentahydrate), Wolfe's vitamin mix (0.1 mg/L Pyridoxine HCl, 0.05 mg/L 4-Aminobenzoic acid, 0.05 mg/L Lipoic acid, 0.05 mg/L Nicotinic Acid, 0.05 mg/L Riboflavin, 0.05 mg/L Thiamine HCl, 0.05 mg/L calcium pantothenate, 0.02 mg/L biotin, 0.02 mg/L Folic Acid, 0.001 mg/L Cyanocobalamin)

Specific Phenotypes

For 24 genes in this experiment

For stress Sodium Chloride in Klebsiella michiganensis M5al

For stress Sodium Chloride across organisms

SEED Subsystems

Subsystem #Specific
Entner-Doudoroff Pathway 2
Glycine Biosynthesis 2
Glycolysis and Gluconeogenesis 2
Glycolysis and Gluconeogenesis, including Archaeal enzymes 2
Phosphate metabolism 2
Trehalose Uptake and Utilization 2
Calvin-Benson cycle 1
Flavodoxin 1
Glycine and Serine Utilization 1
Heat shock dnaK gene cluster extended 1
Na(+) H(+) antiporter 1
Pyruvate metabolism I: anaplerotic reactions, PEP 1
Thioredoxin-disulfide reductase 1
Threonine degradation 1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway #Steps #Present #Specific
L-threonine degradation II 2 2 2
L-threonine degradation III (to methylglyoxal) 3 3 2
NAD phosphorylation and transhydrogenation 2 2 1
aminopropanol phosphate biosynthesis II 4 3 2
NAD phosphorylation and dephosphorylation 3 3 1
Rubisco shunt 10 9 3
Entner-Doudoroff pathway III (semi-phosphorylative) 9 6 2
glycolysis IV 10 8 2
glycolysis V (Pyrococcus) 10 7 2
glycolysis II (from fructose 6-phosphate) 11 11 2
glycolysis III (from glucose) 11 11 2
glycolysis VI (from fructose) 11 8 2
superpathway of L-threonine metabolism 18 18 3
homolactic fermentation 12 12 2
NAD(P)/NADPH interconversion 6 4 1
glycolysis I (from glucose 6-phosphate) 13 13 2
anaerobic energy metabolism (invertebrates, cytosol) 7 5 1
Bifidobacterium shunt 15 13 2
glycerol degradation to butanol 16 12 2
superpathway of glycolysis and the Entner-Doudoroff pathway 17 17 2
superpathway of glucose and xylose degradation 17 17 2
photosynthetic 3-hydroxybutanoate biosynthesis (engineered) 26 19 3
superpathway of hexitol degradation (bacteria) 18 18 2
Entner-Doudoroff pathway I 9 9 1
Entner-Doudoroff pathway II (non-phosphorylative) 9 7 1
1-butanol autotrophic biosynthesis (engineered) 27 19 3
myxochelin A and B biosynthesis 9 2 1
hexitol fermentation to lactate, formate, ethanol and acetate 19 19 2
superpathway of anaerobic sucrose degradation 19 18 2
photorespiration II 10 6 1
superpathway of N-acetylneuraminate degradation 22 22 2
superpathway of cytosolic glycolysis (plants), pyruvate dehydrogenase and TCA cycle 22 18 2
ethene biosynthesis V (engineered) 25 18 2
gluconeogenesis I 13 13 1
superpathway of glycolysis, pyruvate dehydrogenase, TCA, and glyoxylate bypass 26 25 2
Calvin-Benson-Bassham cycle 13 10 1
mixed acid fermentation 16 16 1
superpathway of anaerobic energy metabolism (invertebrates) 17 12 1
oxygenic photosynthesis 17 11 1
heterolactic fermentation 18 16 1
gluconeogenesis II (Methanobacterium thermoautotrophicum) 18 9 1
tRNA charging 21 21 1
Methanobacterium thermoautotrophicum biosynthetic metabolism 56 23 1