Experiment set17IT004 for Pseudomonas stutzeri RCH2
LB with Nickel (II) chloride hexahydrate 1 mM
Group: stressMedia: LB + Nickel (II) chloride hexahydrate (1 mM)
Culturing: psRCH2_ML7c, 48 well microplate; Tecan Infinite F200, Aerobic, at 28 (C), shaken=orbital
By: Kelly on 4/17/2014
Media components: 10 g/L Tryptone, 5 g/L Yeast Extract, 5 g/L Sodium Chloride
Growth plate: 880 A7,A8
Specific Phenotypes
For 5 genes in this experiment
For stress Nickel (II) chloride hexahydrate in Pseudomonas stutzeri RCH2
For stress Nickel (II) chloride hexahydrate across organisms
SEED Subsystems
| Subsystem | #Specific |
|---|---|
| Folate Biosynthesis | 2 |
| Cobalt-zinc-cadmium resistance | 1 |
Metabolic Maps
Color code by fitness: see overview map or list of maps.
Maps containing gene(s) with specific phenotypes:
- Tryptophan metabolism
- Pentose and glucuronate interconversions
- Biosynthesis of steroids
- Lipopolysaccharide biosynthesis
- One carbon pool by folate
- Methane metabolism
- Folate biosynthesis
- Carotenoid biosynthesis - General
- Aminoacyl-tRNA biosynthesis
- Biosynthesis of phenylpropanoids
- Biosynthesis of alkaloids derived from terpenoid and polyketide
MetaCyc Pathways
Pathways that contain genes with specific phenotypes:
| Pathway | #Steps | #Present | #Specific |
|---|---|---|---|
| tetrahydromonapterin biosynthesis | 4 | 3 | 2 |
| dTMP de novo biosynthesis (mitochondrial) | 3 | 3 | 1 |
| tetrahydrofolate biosynthesis I | 3 | 3 | 1 |
| folate transformations III (E. coli) | 9 | 9 | 1 |
| superpathway of tetrahydrofolate biosynthesis | 10 | 8 | 1 |
| folate transformations II (plants) | 11 | 10 | 1 |
| superpathway of tetrahydrofolate biosynthesis and salvage | 12 | 10 | 1 |
| tRNA charging | 21 | 20 | 1 |
| superpathway of chorismate metabolism | 59 | 44 | 1 |