Experiment set17IT003 for Pseudomonas stutzeri RCH2

LB with Nickel (II) chloride hexahydrate 0.9 mM

Group: stress
Media: LB + Nickel (II) chloride hexahydrate (0.9 mM)
Culturing: psRCH2_ML7c, 48 well microplate; Tecan Infinite F200, Aerobic, at 28 (C), shaken=orbital
By: Kelly on 4/17/2014
Media components: 10 g/L Tryptone, 5 g/L Yeast Extract, 5 g/L Sodium Chloride
Growth plate: 880 A5,A6

Specific Phenotypes

For 3 genes in this experiment

For stress Nickel (II) chloride hexahydrate in Pseudomonas stutzeri RCH2

For stress Nickel (II) chloride hexahydrate across organisms

SEED Subsystems

Subsystem	#Specific
Folate Biosynthesis	1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

• Pentose and glucuronate interconversions
• Biosynthesis of steroids
• Tryptophan metabolism
• Lipopolysaccharide biosynthesis
• Carotenoid biosynthesis - General
• Aminoacyl-tRNA biosynthesis

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway	#Steps	#Present	#Specific
tetrahydromonapterin biosynthesis	4	3	1
tRNA charging	21	20	1