Experiment set16IT093 for Desulfovibrio vulgaris Hildenborough JW710

Compare to:

MoLS4 with L-Cysteine 10mM and L-Isoleucine/L-Leucine/L-Valine 10mM as nutrient

Group: nutrient
Media: MoLS4 + L-Cysteine (10 mM) + L-Isoleucine (10 mM) + L-Leucine (10 mM) + L-Valine (10 mM), pH=7.2
Culturing: DvH_JW710, 24 deep-well microplate, Anaerobic, at 30 (C), shaken=0 rpm
By: Valentine on 11/2/2017
Media components: 30 mM Sodium sulfate, 60 mM Sodium D,L-Lactate, 20 mM Ammonium chloride, 30 mM Tris hydrochloride, 0.12 mM EDTA, 1 mM Sodium sulfide nonahydrate, 8 mM Magnesium chloride hexahydrate, 0.6 mM Calcium chloride, 2 mM Potassium phosphate dibasic, 60 uM Iron (II) chloride tetrahydrate, Desulfovibrio trace elements (15 uM Manganese (II) chloride tetrahydrate, 7.8 uM Cobalt chloride hexahydrate, 9 uM Zinc chloride, 1.26 uM Sodium molybdate, 1.92 uM Boric Acid, 2.28 uM Nickel (II) sulfate hexahydrate, 0.06 uM copper (II) chloride dihydrate, 0.21 uM Sodium selenite pentahydrate, 0.144 uM Sodium tungstate dihydrate), Thauer's vitamin mix (0.01 mg/L Pyridoxine HCl, 0.005 mg/L 4-Aminobenzoic acid, 0.005 mg/L Lipoic acid, 0.005 mg/L Nicotinic Acid, 0.005 mg/L Riboflavin, 0.005 mg/L Thiamine HCl, 0.005 mg/L calcium pantothenate, 0.002 mg/L biotin, 0.002 mg/L Folic Acid, 0.0001 mg/L Cyanocobalamin, 0.2 mg/L Choline chloride)

Specific Phenotypes

For 16 genes in this experiment

For nutrient L-Cysteine in Desulfovibrio vulgaris Hildenborough JW710

For nutrient L-Cysteine across organisms

SEED Subsystems

Subsystem #Specific
ABC transporter branched-chain amino acid (TC 3.A.1.4.1) 5
Arginine and Ornithine Degradation 1
Coenzyme A Biosynthesis 1
DNA repair, bacterial MutL-MutS system 1
Potassium homeostasis 1
cAMP signaling in bacteria 1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway #Steps #Present #Specific
pyruvate fermentation to butanoate 7 3 1
superpathway of Clostridium acetobutylicum acidogenic fermentation 9 5 1
L-glutamate degradation VII (to butanoate) 12 4 1
superpathway of Clostridium acetobutylicum acidogenic and solventogenic fermentation 17 8 1