Experiment set16IT066 for Pseudomonas stutzeri RCH2

Lactate-nitrate; anaerobic; dichromate stress sample1

Group: stress
Media: RCH2_Lactate_Nitrate_YE + Potassium dichromate (240 uM), pH=7
Culturing: psRCH2_ML7e, hungate, Anaerobic, at 30 (C), shaken=200 rpm
Growth: about 5.1 generations
By: Mike Adams on 5/12/2014

Specific Phenotypes

For 10 genes in this experiment

For stress Potassium dichromate in Pseudomonas stutzeri RCH2

For stress Potassium dichromate across organisms

SEED Subsystems

Subsystem	#Specific
DNA repair, bacterial RecFOR pathway	2
DNA repair, UvrABC system	1
Denitrification	1
Dissimilatory nitrite reductase	1
Glutamate dehydrogenases	1
Glutamine, Glutamate, Aspartate and Asparagine Biosynthesis	1
Heme biosynthesis orphans	1
Molybdenum cofactor biosynthesis	1
Na(+) H(+) antiporter	1
Proteolysis in bacteria, ATP-dependent	1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

• Nitrogen metabolism
• Glutamate metabolism
• Cysteine metabolism
• Arginine and proline metabolism

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway	#Steps	#Present	#Specific
thiosulfate disproportionation IV (rhodanese)	1	1	1
L-glutamate degradation I	1	1	1
L-cysteine degradation III	2	1	1
L-alanine degradation II (to D-lactate)	3	3	1
ethene biosynthesis IV (engineered)	3	1	1
nitrite-dependent anaerobic methane oxidation	3	1	1
ammonia oxidation II (anaerobic)	3	1	1
nitrate reduction I (denitrification)	5	4	1
nitrate reduction VII (denitrification)	5	3	1
sulfide oxidation IV (mitochondria)	5	2	1
nitrifier denitrification	6	3	1
L-glutamate degradation XI (reductive Stickland reaction)	7	4	1
4-aminobutanoate degradation V	7	3	1
L-glutamate degradation V (via hydroxyglutarate)	10	7	1
methylaspartate cycle	19	9	1