Experiment set16IT049 for Bacteroides thetaiotaomicron VPI-5482

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Supernatant; Agrobacterium rhizogenes K599 grown in Agro_defined_trehalose (~7.5 mM 3-keto-trehalose) 0.5x carbon source

Group: carbon source
Media: Varel_Bryant_medium + supernatant from Agrobacterium rhizogenes K599 grown in Agro_defined_trehalose (~7.5 mM 3-keto-trehalose) (0.5 x)
Culturing: Btheta_ML6a, 24-well transparent microplate, Anaerobic, at 37 (C), shaken=0 rpm
By: Valentine on 6/30/20
Media components: 15 uM Hemin, 134 uM L-Methionine, 15 uM Iron (II) sulfate heptahydrate, 8.25 mM L-Cysteine, 23.8 mM Sodium bicarbonate, Mineral 3B solution (6.6 mM Potassium phosphate monobasic, 15.4 mM Sodium Chloride, 98 uM Magnesium chloride hexahydrate, 176.5 uM Calcium chloride dihydrate, 4.2 uM Cobalt chloride hexahydrate, 50.5 uM Manganese (II) chloride tetrahydrate, 9.3 mM Ammonium chloride, 1.75 mM Sodium sulfate)

Specific Phenotypes

For 1 genes in this experiment

For carbon source Supernatant; Agrobacterium rhizogenes K599 grown in Agro_defined_trehalose (~7.5 mM 3-keto-trehalose) in Bacteroides thetaiotaomicron VPI-5482

For carbon source Supernatant; Agrobacterium rhizogenes K599 grown in Agro_defined_trehalose (~7.5 mM 3-keto-trehalose) across organisms

SEED Subsystems

Subsystem #Specific
De Novo Purine Biosynthesis 1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway #Steps #Present #Specific
5-aminoimidazole ribonucleotide biosynthesis II 5 5 1
5-aminoimidazole ribonucleotide biosynthesis I 5 5 1
superpathway of 5-aminoimidazole ribonucleotide biosynthesis 6 6 1
superpathway of purine nucleotides de novo biosynthesis I 21 21 1
superpathway of purine nucleotides de novo biosynthesis II 26 23 1
superpathway of histidine, purine, and pyrimidine biosynthesis 46 42 1